BACE Practice Quiz 5 Biotechnology Skills and All Correct Answers .

An assay requires 20 µL of a reagent. Which of the following micropipettes should be used to dispense the most accurate volume? - Answer 10 - 100 µL What is the volume in mL in the graduated cylinder? Record the correct amount rounded to one decimal place. - Answer 43.0 Which steps should you take to ensure accuracy and avoid errors when measuring the mass of a powdered substance? - Answer Tare the balance with a container, and avoid any drafts or vibrations. Which of the following is the correct method to make 500 mL of 20% glucose? - Answer Dissolve the appropriate amount of glucose in a small volume of dH2O, then adjust the volume to 500 mL While preparing a solution, a technician uses a pH meter to measure the pH. The protocol requires that the pH of the solution be 8.25, however the reading given by the meter is 9.15. Which of the following solutions should the technician use to adjust the pH to 8.25? - Answer Hydrochloric acid (HCl) What is the primary purpose of performing a serial dilution in a laboratory setting? - Answer To reduce the concentration of a substance in order to obtain measurable concentrations. What is the first step in identifying a reagent before use in a laboratory setting? - Answer Checking the label on the container for chemical name, concentration, and expiration date. While preparing an agarose gel, the solution doesn't solidify after cooling. What might be the issue affecting the performance of the agarose reagent? - Answer The agarose was not heated long enough to fully dissolve. What is the proper way to disinfect a work surface before starting aseptic techniques? - Answer Clean the surface with 70% isopropyl alcohol Match the aseptic technique practice on the left with its purpose on the right. Falme Sterilizing instruments