AAB BASIC KNOWLEDGE ACTUAL EXAM
PAPER 2026 QUESTIONS WITH ANSWERS
GRADED A+
● 3 types of pipettes. Answer: 1. automated
2. semi-automated
3. glass
● Automated pipettes. Answer: frequently used to prepare many samples
for analysis and can be an integral part of automated analyzer
● Semi-automated micropipettes. Answer: contain or deliver small
volumes of liquid ranging from 1 to 1000ul
● 2 types of displacement for semi-automated pipettes. Answer: air
displacement: forward & reverse
positive displacement
● Types of glass pipettes. Answer: -transfer/volumetric
-measuring/graduated
-to contain (TC) or rinse-out pipettes
,-to deliver (TD) pipettes
-to deliver/blow-out
● Transfer/volumetric pipettes. Answer: have a bulb in the middle of the
pipette with a tapered delivery tip
● Measuring/Graduated pipette. Answer: uniform in diameter with a
tapered delivery tip
● To Contain (TC) or Rinse-out pipettes. Answer: must be rinsed with
the appropriate solvent after the initial liquid has been drained from
these pipettes
● To Deliver (TD) pipettes. Answer: filled & allowed to drain by gravity
-must be vertically and the tip placed against the side of the receiving
vessel
● To Deliver/Blow Out pipette. Answer: are filled and allowed to drain,
after which the remaining fluid in the tip is blown out.
-easily identified by the 2 frosted bands near mouthpiece of the pipette
● Temperature of Heat Blocks/Water baths. Answer: 36C-38C
● Room Temperature. Answer: 20C-24C
, ● Temperature of Refrigerators. Answer: Laboratory 2C-8C
Blood Banking 1C-6C
● Temperature of Freezers. Answer: Laboratory <18C
Blood Bank <65C
● Bright-field microscopy. Answer: -simplest of all the light microscopy
-sample illumination is via transmitted white light illuminated from
below and observed from above
-most commonly used microscope
● Dark-field microscopy. Answer: -technique for improving the contrast
of unstained, transparent specimen
-uses a carefully aligned light source to minimize the quantity of directly
transmitted light entering the image plane, collecting only the light
scattered by sample
● Electron microscopy. Answer: -electrons illuminate a specimen and
create an enlarged image
-uses electrostatic and electromagnetic lenses in forming the image by
controlling the electron beam to focus it at a specific plane relative to the
specimen in a manner similar to how a light microscope uses glass
lenses to focus light on or through a specimen to form an image
PAPER 2026 QUESTIONS WITH ANSWERS
GRADED A+
● 3 types of pipettes. Answer: 1. automated
2. semi-automated
3. glass
● Automated pipettes. Answer: frequently used to prepare many samples
for analysis and can be an integral part of automated analyzer
● Semi-automated micropipettes. Answer: contain or deliver small
volumes of liquid ranging from 1 to 1000ul
● 2 types of displacement for semi-automated pipettes. Answer: air
displacement: forward & reverse
positive displacement
● Types of glass pipettes. Answer: -transfer/volumetric
-measuring/graduated
-to contain (TC) or rinse-out pipettes
,-to deliver (TD) pipettes
-to deliver/blow-out
● Transfer/volumetric pipettes. Answer: have a bulb in the middle of the
pipette with a tapered delivery tip
● Measuring/Graduated pipette. Answer: uniform in diameter with a
tapered delivery tip
● To Contain (TC) or Rinse-out pipettes. Answer: must be rinsed with
the appropriate solvent after the initial liquid has been drained from
these pipettes
● To Deliver (TD) pipettes. Answer: filled & allowed to drain by gravity
-must be vertically and the tip placed against the side of the receiving
vessel
● To Deliver/Blow Out pipette. Answer: are filled and allowed to drain,
after which the remaining fluid in the tip is blown out.
-easily identified by the 2 frosted bands near mouthpiece of the pipette
● Temperature of Heat Blocks/Water baths. Answer: 36C-38C
● Room Temperature. Answer: 20C-24C
, ● Temperature of Refrigerators. Answer: Laboratory 2C-8C
Blood Banking 1C-6C
● Temperature of Freezers. Answer: Laboratory <18C
Blood Bank <65C
● Bright-field microscopy. Answer: -simplest of all the light microscopy
-sample illumination is via transmitted white light illuminated from
below and observed from above
-most commonly used microscope
● Dark-field microscopy. Answer: -technique for improving the contrast
of unstained, transparent specimen
-uses a carefully aligned light source to minimize the quantity of directly
transmitted light entering the image plane, collecting only the light
scattered by sample
● Electron microscopy. Answer: -electrons illuminate a specimen and
create an enlarged image
-uses electrostatic and electromagnetic lenses in forming the image by
controlling the electron beam to focus it at a specific plane relative to the
specimen in a manner similar to how a light microscope uses glass
lenses to focus light on or through a specimen to form an image
