UAMS HEMATOLOGY PRACTICE TEST EXAM QUESTIONS
AND ANSWERS GRADED A+
✔✔What is paroxysmal nocturnal hemoglbinuria? - ✔✔Clonal stem cell disorder with
cells sensitive to complement lysis
✔✔What are the laboratory results for PNH? - ✔✔Red urine, + urine hemosiderin, +
sucrose, + Ham's test
✔✔What is the older screening test for PNH? - ✔✔Sugar water test
✔✔What is the older confirmatory test for Paroxysmal Nocturnal Hemoglobinuria -
✔✔Ham's test
✔✔What test distinguishes hereditary spherocytosis from warm autoimmune hemolytic
anemia? - ✔✔DAT
✔✔What are the elements that must be present for hemolysis to occur in the positive
tube of the Ham's test? - ✔✔Acid, complement, patient RBC lacking the DAF and HRF
Proteins
✔✔What is the current, most accurate testing methodology for PNH? - ✔✔Flow
cytometry with CD55 & 59
✔✔What happens when taking oxidant drugs with G6PD deficiency? - ✔✔Oxidant
drugs cause hemoglobin to denature and precipitate
✔✔What are the laboratory results for G6PD deficiency? - ✔✔Heinz Bodies seen with
supravital stain, polychromasia, increased retic, anisocytosis
✔✔Why must G6PD testing be done after the hemolytic crisis has passed? - ✔✔During
hemolytic crisis the BM is producing new cells at an accelerated rate. Retics have more
of the enzyme than mature cells
✔✔What is seen on a peripheral smear in warm autoimmune hemolytic anemia? -
✔✔Spherocytes & polychromasia
✔✔Which anemia occurs after an infection with infectious mono or mycoplasma? -
✔✔Cold autoimmune hemolytic anemia
✔✔What is seen on a peripheral smear in cold autoimmune hemolytic anemia? -
✔✔Agglutination
, ✔✔What happens in pyruvate kinase deficiency? - ✔✔Reduced ATP production results
in alterations of the RBC membrane leading to cell dehydration (echinocytes)
✔✔What is the defect in sickle cell anemia? - ✔✔Valine is substituted for glutamic acid
on the sixth position of the Beta chain
✔✔What test is used in sickle cell but cannot differentiate from disease and trait? -
✔✔Sickle solubility
✔✔What is the confirmatory test for sickle cell? - ✔✔Hemoglobin electrophoresis
✔✔What is seen on peripheral smear in hemoglobin C disease? - ✔✔Hgb C crystals,
target cells
✔✔What is increased in hemochromatosis? - ✔✔Iron absorption
✔✔What anemia is found in mononucleosis? - ✔✔Cold agglutinin
✔✔What percent of transferrin is bound to iron? - ✔✔30%
✔✔How is intravascular distinguished from extravascular hemolysis? - ✔✔Urine
hemosiderin
✔✔What other tests are utilized in the detection of hemolytic anemia? - ✔✔Bilirubin,
haptoglobin, urine hemosiderin
✔✔What is the retic value for anemias? - ✔✔increased in hemolytic anemias,
decreased in nutritional deficiency until needs are met then increased, normal or
decreased in aplastic anemia
✔✔Which of the anemias are life threatening? - ✔✔Alpha thalassemia major
✔✔How can IDA and thalassemia be differentiated? - ✔✔Hemoglobin electrophoresis
✔✔How can Hgb A with Hgb S be confirmed? - ✔✔Hemoglobin electrophoresis using
citrate agar
✔✔How can IDA and anemia of chronic disease be differentiated? - ✔✔Ferritin & TIBC
✔✔What will the bone marrow of anemia indicate? - ✔✔Erythroid hyperplasia 1:1
✔✔What is the purpose of the schilling test? - ✔✔Detects malabsorption of B12 found
in pernicious anemia
AND ANSWERS GRADED A+
✔✔What is paroxysmal nocturnal hemoglbinuria? - ✔✔Clonal stem cell disorder with
cells sensitive to complement lysis
✔✔What are the laboratory results for PNH? - ✔✔Red urine, + urine hemosiderin, +
sucrose, + Ham's test
✔✔What is the older screening test for PNH? - ✔✔Sugar water test
✔✔What is the older confirmatory test for Paroxysmal Nocturnal Hemoglobinuria -
✔✔Ham's test
✔✔What test distinguishes hereditary spherocytosis from warm autoimmune hemolytic
anemia? - ✔✔DAT
✔✔What are the elements that must be present for hemolysis to occur in the positive
tube of the Ham's test? - ✔✔Acid, complement, patient RBC lacking the DAF and HRF
Proteins
✔✔What is the current, most accurate testing methodology for PNH? - ✔✔Flow
cytometry with CD55 & 59
✔✔What happens when taking oxidant drugs with G6PD deficiency? - ✔✔Oxidant
drugs cause hemoglobin to denature and precipitate
✔✔What are the laboratory results for G6PD deficiency? - ✔✔Heinz Bodies seen with
supravital stain, polychromasia, increased retic, anisocytosis
✔✔Why must G6PD testing be done after the hemolytic crisis has passed? - ✔✔During
hemolytic crisis the BM is producing new cells at an accelerated rate. Retics have more
of the enzyme than mature cells
✔✔What is seen on a peripheral smear in warm autoimmune hemolytic anemia? -
✔✔Spherocytes & polychromasia
✔✔Which anemia occurs after an infection with infectious mono or mycoplasma? -
✔✔Cold autoimmune hemolytic anemia
✔✔What is seen on a peripheral smear in cold autoimmune hemolytic anemia? -
✔✔Agglutination
, ✔✔What happens in pyruvate kinase deficiency? - ✔✔Reduced ATP production results
in alterations of the RBC membrane leading to cell dehydration (echinocytes)
✔✔What is the defect in sickle cell anemia? - ✔✔Valine is substituted for glutamic acid
on the sixth position of the Beta chain
✔✔What test is used in sickle cell but cannot differentiate from disease and trait? -
✔✔Sickle solubility
✔✔What is the confirmatory test for sickle cell? - ✔✔Hemoglobin electrophoresis
✔✔What is seen on peripheral smear in hemoglobin C disease? - ✔✔Hgb C crystals,
target cells
✔✔What is increased in hemochromatosis? - ✔✔Iron absorption
✔✔What anemia is found in mononucleosis? - ✔✔Cold agglutinin
✔✔What percent of transferrin is bound to iron? - ✔✔30%
✔✔How is intravascular distinguished from extravascular hemolysis? - ✔✔Urine
hemosiderin
✔✔What other tests are utilized in the detection of hemolytic anemia? - ✔✔Bilirubin,
haptoglobin, urine hemosiderin
✔✔What is the retic value for anemias? - ✔✔increased in hemolytic anemias,
decreased in nutritional deficiency until needs are met then increased, normal or
decreased in aplastic anemia
✔✔Which of the anemias are life threatening? - ✔✔Alpha thalassemia major
✔✔How can IDA and thalassemia be differentiated? - ✔✔Hemoglobin electrophoresis
✔✔How can Hgb A with Hgb S be confirmed? - ✔✔Hemoglobin electrophoresis using
citrate agar
✔✔How can IDA and anemia of chronic disease be differentiated? - ✔✔Ferritin & TIBC
✔✔What will the bone marrow of anemia indicate? - ✔✔Erythroid hyperplasia 1:1
✔✔What is the purpose of the schilling test? - ✔✔Detects malabsorption of B12 found
in pernicious anemia
