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BIOL 271 / BIOL271 Final Exam: (Latest 2026/ 2027 Update) 100% Verified Questions & Answers | Grade A

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BIOL 271 / BIOL271 Final Exam: (Latest 2026/ 2027 Update) 100% Verified Questions & Answers | Grade A Q: When performing a quadrant streak, the sample is spread across the plate in such a way to form what? Answer: A dilution gradient. Q: A dilution gradient is formed when carrying out what generalize plating strategy? Answer: Phase-Dilution streak Q: In what phase of dilution streak would you expect to find the lowest concentration of bacteria. P2 or P4? Answer: P4. The phases concentration rank from P1 being the highest, then P2, P3, and P4. Q: True or False. When performing a dilution streak a new (or sterilized) loop is not required for each phase as long as the bacterial culture is pure. Answer: False Q: The number of phases (3 vs. 4) and/or the number of times a loop passes through a previous phase (once vs. multiple) is acceptable provided what happens? Answer: The resulting gradient has growth of the individual colonies in it Q: True or False. To restrict the growth of a pathogenic microbe a researcher might decrease an incubator from 37°C to 25°C. Answer: True Q: When given an unknown bacterial sample the first step is to expand the current bacterial population. Which form of media best suites this need? Why? 1. MSA agar 2. LB media 3. MacConkey agar 4. Columbia CNA agar Answer: LB media. All other options are forms of selective media which means they would potentially inhibit the growth of the unknown sample. Q: True or False. Only directly ingesting the pre-formed clostridium toxin (neither the bacteria nor its spores) will cause disease in adults. Answer: True Q: True or False. There are currently no cures for tetanus. Answer: True Q: The alpha-toxin perfringolysin is associated with which medical condition caused by Gram-positive anaerobic bacteria? Tetanus Leprosy Botulism Gas gangrene Answer: Gas gangrene Q: True or False. Pneumonic plague is often characterized by painfully swollen lymph nodes. Answer: False Q: True or False. Bubonic plague is often characterized by painfully swollen lymph nodes. Answer: True Q: Identify the following disease: (image of hand with large black open sore on base of thumb) Gas gangrene Frost bite Anthrax Tetanus Answer: Anthrax Q: True or False. Chlamydia trachomatis can be grown on an agar plate alone

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BIOLl271l /l BIOL271l Finall Exam:l
(Latestl 2026/l 2027l Update)l 100%l
Verifiedl Questionsl &l Answersl |l Gradel A



Q:l Whenl performingl al quadrantl streak,l thel samplel isl spreadl acrossl thel
platel inl suchl al wayl tol forml what?


Answer:
Al dilutionl gradient.




Q:l Al dilutionl gradientl isl formedl whenl carryingl outl whatl generalizel
platingl strategy?


Answer:
Phase-Dilutionl streak




Q:l Inl whatl phasel ofl dilutionl streakl wouldl youl expectl tol findl thel lowestl
concentrationl ofl bacteria.l P2l orl P4?


Answer:
P4.
Thel phasesl concentrationl rankl froml P1l beingl thel highest,l thenl P2,l P3,l andl
P4.

,Q:l Truel orl False.l Whenl performingl al dilutionl streakl al newl (orl sterilized)l
loopl isl notl requiredl forl eachl phasel asl longl asl thel bacteriall culturel isl pure.


Answer:
False




Q:l Thel numberl ofl phasesl (3l vs.l 4)l and/orl thel numberl ofl timesl al loopl
passesl throughl al previousl phasel (oncel vs.l multiple)l isl acceptablel providedl
whatl happens?


Answer:
Thel resultingl gradientl hasl growthl ofl thel individuall coloniesl inl it




Q:l Truel orl False.l Tol restrictl thel growthl ofl al pathogenicl microbel al
researcherl mightl decreasel anl incubatorl froml 37°Cl tol 25°C.


Answer:
True




Q:l Whenl givenl anl unknownl bacteriall samplel thel firstl stepl isl tol expandl
thel currentl bacteriall population.l Whichl forml ofl medial bestl suitesl thisl
need?l Why?
1.l MSAl agar

,2.l LBl media
3.l MacConkeyl agar
4.l Columbial CNAl agar


Answer:
LBl media.l Alll otherl optionsl arel formsl ofl selectivel medial whichl meansl
theyl wouldl potentiallyl inhibitl thel growthl ofl thel unknownl sample.




Q:l Truel orl False.l Onlyl directlyl ingestingl thel pre-formedl clostridiuml toxinl
(neitherl thel bacterial norl itsl spores)l willl causel diseasel inl adults.


Answer:
True




Q:l Truel orl False.l Therel arel currentlyl nol curesl forl tetanus.

Answer:
True




Q:l Thel alpha-toxinl perfringolysinl isl associatedl withl whichl medicall
conditionl causedl byl Gram-positivel anaerobicl bacteria?
Tetanus
Leprosy
Botulism
Gasl gangrene

, Answer:
Gasl gangrene




Q:l Truel orl False.l Pneumonicl plaguel isl oftenl characterizedl byl painfullyl
swollenl lymphl nodes.


Answer:
False




Q:l Truel orl False.l Bubonicl plaguel isl oftenl characterizedl byl painfullyl
swollenl lymphl nodes.


Answer:
True




Q:l Identifyl thel followingl disease:l (imagel ofl handl withl largel blackl openl
sorel onl basel ofl thumb)
Gasl gangrene
Frostl bite
Anthrax
Tetanus


Answer:
Anthrax

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