GMS 6552 EXAM Questions AND Correct Answers
6PF2K - ✔✔- activated by PKB/Akt
- stimulates glycolysis
Activation of latent TGF-b - ✔✔- mature TGF-b cleaved from latent TGF-b by
plasmin
- mature TGF-b then able to bind TGF-b receptor
Adenylate cyclase - ✔✔- activated by GPCR
- cAMP as second messenger
- activate PKA
Agonist binding - ✔✔- does not stabilize GPCR active conformation
- suggests weak link between agonist bind pocket and G-protein binding surface
Alpha subunit - ✔✔- binds GTP converting to GDP
Anoikis - ✔✔- form of apoptosis due to cell detachment
- killed when cell detaches from designated location and travels to spot it is no
supposed to be
Antagonist inhibtion - ✔✔- do not change GPCR conformation but inactivate
receptor
- may prevent binding of agonist or inverse agonist
,Antagonists - ✔✔- may or may not work by binding same site agonists bind to
- competative bind same site
Anti-apoptotic BCL-2 proteins - ✔✔- BCL-2
- BCL-w
- BCL-xl
- A1
- MCL-1
Apoptosis - ✔✔- type I programmed cell death
- cell shrinkage
- nuclear condensation
- membrane blebbing
- loss of adhesion
- cell suicide
- intrinsic
Apoptosome - ✔✔- binds cytochrome C from mitochondria
- activates caspase 9
- contains Apaf-1 (apoptotic protease activating factor-1)
Augophagy - ✔✔- type II programmed cell death
, - catabolic process involving degradation of cellular compenents by lysosomal
machinery
- intrinsic
b-arrestin proteins - ✔✔- conserved and ubiquitous
- co-regulators of GPCRs and other receptors
- densensitize or trigger receptor endocytosis
- signal transduction or biased agonsim
Blotting - ✔✔- primary antibody binds to target protein
- secondary antibody binds to primary antibody, joined to an enzyme for
detection (horseraddish peroxidase)
- add detection agent to allow for detectable signal
Canonical pathway - ✔✔- rapid & transient
- independent of protein synthesis
- respond to numerous stimuli
- diverse function
- IkBa degradation by proteasome
Canonical receptors - ✔✔- extracellular
- TLR4
- IL-1R
6PF2K - ✔✔- activated by PKB/Akt
- stimulates glycolysis
Activation of latent TGF-b - ✔✔- mature TGF-b cleaved from latent TGF-b by
plasmin
- mature TGF-b then able to bind TGF-b receptor
Adenylate cyclase - ✔✔- activated by GPCR
- cAMP as second messenger
- activate PKA
Agonist binding - ✔✔- does not stabilize GPCR active conformation
- suggests weak link between agonist bind pocket and G-protein binding surface
Alpha subunit - ✔✔- binds GTP converting to GDP
Anoikis - ✔✔- form of apoptosis due to cell detachment
- killed when cell detaches from designated location and travels to spot it is no
supposed to be
Antagonist inhibtion - ✔✔- do not change GPCR conformation but inactivate
receptor
- may prevent binding of agonist or inverse agonist
,Antagonists - ✔✔- may or may not work by binding same site agonists bind to
- competative bind same site
Anti-apoptotic BCL-2 proteins - ✔✔- BCL-2
- BCL-w
- BCL-xl
- A1
- MCL-1
Apoptosis - ✔✔- type I programmed cell death
- cell shrinkage
- nuclear condensation
- membrane blebbing
- loss of adhesion
- cell suicide
- intrinsic
Apoptosome - ✔✔- binds cytochrome C from mitochondria
- activates caspase 9
- contains Apaf-1 (apoptotic protease activating factor-1)
Augophagy - ✔✔- type II programmed cell death
, - catabolic process involving degradation of cellular compenents by lysosomal
machinery
- intrinsic
b-arrestin proteins - ✔✔- conserved and ubiquitous
- co-regulators of GPCRs and other receptors
- densensitize or trigger receptor endocytosis
- signal transduction or biased agonsim
Blotting - ✔✔- primary antibody binds to target protein
- secondary antibody binds to primary antibody, joined to an enzyme for
detection (horseraddish peroxidase)
- add detection agent to allow for detectable signal
Canonical pathway - ✔✔- rapid & transient
- independent of protein synthesis
- respond to numerous stimuli
- diverse function
- IkBa degradation by proteasome
Canonical receptors - ✔✔- extracellular
- TLR4
- IL-1R
