AAB Medical Technologist – Chemistry Exam Prep 2025/2026 |
Complete Study Guide with Verified Practice Questions & Answers
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - correct answerNitrogen
Which of the proteins is soluble in water? - correct answerAlbumin
How are amino acids joined together to form a chainlike structure? - correct
answerPeptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - correct
answerAlteration of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - correct
answerCystinuria
These aminoacidopathies are caused by an enzyme deficiency: - correct answerMaple
syrup disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - correct answerFive
Which reagent is employed in the serum protein determination? - correct answerBiuret
Why should hemolyzed serum not be used for total protein analysis? - correct
answerHemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - correct answerIt is relatively
free from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - correct answerFollows Beer's law
for a reasonable range of protein, depends on the presence of at least two peptide
linkages, and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
correct answerThere is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - correct answerAlbumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
,Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - correct answer3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - correct answerActive cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
correct answerMonoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - correct
answerCarbohydrates
All of the following are glycoproteins: - correct answerCeruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - correct answerCryoglobulin
Which one of the following applies to cryoglobulins? - correct answerThey are
temperature-sensitive proteins
Which of the following statements is true of albumin? - correct answerIt is produced in
the liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - correct
answerDown syndrome
Which one of the following proteins transports iron? - correct answerTransferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - correct answerBlood
urea nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - correct
answerAmmonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - correct answerThe method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- correct answerElevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - correct answerJaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - correct answerAlkaline picrate
, The creatinine clearance test is based on which assumption? - correct
answerCreatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate?
(GFR) - correct answerCreatinine
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum
creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance,
assuming average body surface area? - correct answerGFR = ([UCr x V]/SCr)/1440
64 mL/minute = [(240 x 770)/2]/1440
(1440 minutes in 24 hours)
Which disease state is associated with an elevation of serum uric acid? - correct
answerGout
Which reagent is employed in the alkaline oxidation of uric acid? - correct
answerPhosphotungstic acid
What does the uricase method for uric acid depend on? - correct answerUV absorption
at 290 nm before/after treatment with uricase
Which one of the following analytes is used as a prognostic indicator for liver failure? -
correct answerAmmonia
Which disaccharide is composed of two glucose molecules? - correct answerMaltose
What term is used to describe the process of glycogen degradation? - correct
answerGlycogenolysis
What is the renal threshold for glucose? - correct answer160-180 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? - correct
answerInsulin
Which one of the following hormones is considered a glucocorticoid? - correct
answerCortisol
A 40-year-old male has a randome glucose of 470 mg/dL. This lab finding is consistent
with which one of the following clinical conditions? - correct answerDiabetes mellitus
Which one of the following diseases is associated with hypoglycemia? - correct
answervon Gierke's disease
What is the specimen of choice for glucose determinations? - correct answerFluorinated
plasma
Complete Study Guide with Verified Practice Questions & Answers
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - correct answerNitrogen
Which of the proteins is soluble in water? - correct answerAlbumin
How are amino acids joined together to form a chainlike structure? - correct
answerPeptide bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - correct
answerAlteration of tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - correct
answerCystinuria
These aminoacidopathies are caused by an enzyme deficiency: - correct answerMaple
syrup disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - correct answerFive
Which reagent is employed in the serum protein determination? - correct answerBiuret
Why should hemolyzed serum not be used for total protein analysis? - correct
answerHemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - correct answerIt is relatively
free from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - correct answerFollows Beer's law
for a reasonable range of protein, depends on the presence of at least two peptide
linkages, and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
correct answerThere is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - correct answerAlbumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
,Gamma 14.8% 1.1 g/dL
What is the A/G ratio? - correct answer3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - correct answerActive cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
correct answerMonoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - correct
answerCarbohydrates
All of the following are glycoproteins: - correct answerCeruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - correct answerCryoglobulin
Which one of the following applies to cryoglobulins? - correct answerThey are
temperature-sensitive proteins
Which of the following statements is true of albumin? - correct answerIt is produced in
the liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - correct
answerDown syndrome
Which one of the following proteins transports iron? - correct answerTransferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - correct answerBlood
urea nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - correct
answerAmmonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - correct answerThe method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- correct answerElevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - correct answerJaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - correct answerAlkaline picrate
, The creatinine clearance test is based on which assumption? - correct
answerCreatinine passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate?
(GFR) - correct answerCreatinine
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum
creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance,
assuming average body surface area? - correct answerGFR = ([UCr x V]/SCr)/1440
64 mL/minute = [(240 x 770)/2]/1440
(1440 minutes in 24 hours)
Which disease state is associated with an elevation of serum uric acid? - correct
answerGout
Which reagent is employed in the alkaline oxidation of uric acid? - correct
answerPhosphotungstic acid
What does the uricase method for uric acid depend on? - correct answerUV absorption
at 290 nm before/after treatment with uricase
Which one of the following analytes is used as a prognostic indicator for liver failure? -
correct answerAmmonia
Which disaccharide is composed of two glucose molecules? - correct answerMaltose
What term is used to describe the process of glycogen degradation? - correct
answerGlycogenolysis
What is the renal threshold for glucose? - correct answer160-180 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? - correct
answerInsulin
Which one of the following hormones is considered a glucocorticoid? - correct
answerCortisol
A 40-year-old male has a randome glucose of 470 mg/dL. This lab finding is consistent
with which one of the following clinical conditions? - correct answerDiabetes mellitus
Which one of the following diseases is associated with hypoglycemia? - correct
answervon Gierke's disease
What is the specimen of choice for glucose determinations? - correct answerFluorinated
plasma
