BIO 260- DNA Replication
1. DNA Replication: Also known as DNA synthesis, the process in which DNA makes a duplicate copy of itself.
2. One error/million bp leads to 6400 mistakes every time a cell divides, which
would be catastrophic.: Why does DNA replication need to be extremely accurate
3. A high speed
-E. coli replicates its DNA at a rate of 1000 nucleotides/second.: Replication also takes
place at:
4. Theta Replication: Replication in prokaryotes of circular DNA that is initiated by the unwinding of the two
nucleotide strands, producing a replication bubble. Unwinding continues at one or both ends of the bubble, making
it progressively larger.
-DNA replication on both of the template strands is simultaneous with unwinding until the two replication forks meet.
5. Origin of Replication (Ori): specific sequence indicating a start for replication
- is common in prokaryotes/bacteria
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, 6. Replication Bubble: a region of DNA, in front of the replication fork, where helicase has unwound the double
helix
- contains 2 replication forks
- bidirectional (<-->)
7. Linear Replication: -occurs in Eukaryotes
-multiple origin sites to start replication process
8. Autonomic Replication Sites (ARSs): The name of the origin site in linear replication
- are not located in genes, but near genes that code for proteins important for replication.
ate, act as
9. semiconservative replication: Method of DNA replication in which parental strands separ
templates, and produce molecules of DNA with one parental DNA strand and one new DNA strand
10. 1) Initiation and Unwinding
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1. DNA Replication: Also known as DNA synthesis, the process in which DNA makes a duplicate copy of itself.
2. One error/million bp leads to 6400 mistakes every time a cell divides, which
would be catastrophic.: Why does DNA replication need to be extremely accurate
3. A high speed
-E. coli replicates its DNA at a rate of 1000 nucleotides/second.: Replication also takes
place at:
4. Theta Replication: Replication in prokaryotes of circular DNA that is initiated by the unwinding of the two
nucleotide strands, producing a replication bubble. Unwinding continues at one or both ends of the bubble, making
it progressively larger.
-DNA replication on both of the template strands is simultaneous with unwinding until the two replication forks meet.
5. Origin of Replication (Ori): specific sequence indicating a start for replication
- is common in prokaryotes/bacteria
1/4
, 6. Replication Bubble: a region of DNA, in front of the replication fork, where helicase has unwound the double
helix
- contains 2 replication forks
- bidirectional (<-->)
7. Linear Replication: -occurs in Eukaryotes
-multiple origin sites to start replication process
8. Autonomic Replication Sites (ARSs): The name of the origin site in linear replication
- are not located in genes, but near genes that code for proteins important for replication.
ate, act as
9. semiconservative replication: Method of DNA replication in which parental strands separ
templates, and produce molecules of DNA with one parental DNA strand and one new DNA strand
10. 1) Initiation and Unwinding
2/4
