BIO 260 Technologies
1. PCR (Polymerase Chain Reaction): Method for Amplifying DNA
2. -Taq polymerase
-dNTPs
-primers
-MgCl cofactors
-Template DNA: Ingredients of PCR
3. q-PCR/Real Time PCR: -quantitative PCR
-indicates the number of gene copies present in the original sample.
-a dye or "probe" is used to measure amount of DNA after each cycle
- a brighter glow indicates more starting materials present in PCR
4. -Recombinant Cloning
1/4
, -Sequencing (3 types: Sanger, NextGen, and Nanopore)
-Gene Therapy (using recombinant retroviruses for good): What are some fun things that
we can do with our millions of DNA Copies
5. Recombinant Cloning; Genetic Engineering: -Digest plasmid and DNA fragment w/ restriction
enzymes
generates "sticky ends"
-Recombine the 2 differnt DNA molecules using DNA ligase (hence Recombinant cloning)
-Transform into model org. (E. coli or yeast)
-bacteria express the gene
-"cell factories"
-insulin, interferon, clotting factors, Hep. B vaccine
-Genetically Modified Organisms (GMOs)
6. Genetic Engineering of BT Corn: Bt corn and unmodified corn growing in alternate strips
-prevents corn borer larvae from developing resistance to the
genetically engineered Bt toxin
2/4
1. PCR (Polymerase Chain Reaction): Method for Amplifying DNA
2. -Taq polymerase
-dNTPs
-primers
-MgCl cofactors
-Template DNA: Ingredients of PCR
3. q-PCR/Real Time PCR: -quantitative PCR
-indicates the number of gene copies present in the original sample.
-a dye or "probe" is used to measure amount of DNA after each cycle
- a brighter glow indicates more starting materials present in PCR
4. -Recombinant Cloning
1/4
, -Sequencing (3 types: Sanger, NextGen, and Nanopore)
-Gene Therapy (using recombinant retroviruses for good): What are some fun things that
we can do with our millions of DNA Copies
5. Recombinant Cloning; Genetic Engineering: -Digest plasmid and DNA fragment w/ restriction
enzymes
generates "sticky ends"
-Recombine the 2 differnt DNA molecules using DNA ligase (hence Recombinant cloning)
-Transform into model org. (E. coli or yeast)
-bacteria express the gene
-"cell factories"
-insulin, interferon, clotting factors, Hep. B vaccine
-Genetically Modified Organisms (GMOs)
6. Genetic Engineering of BT Corn: Bt corn and unmodified corn growing in alternate strips
-prevents corn borer larvae from developing resistance to the
genetically engineered Bt toxin
2/4
