BIOTECH 2M03 – Molecular Biology
TEST 2 (version 1) - Nov 12, 2012 - Answers
Name: _______________________________________
MacID:
Instructions:
1. Tests written in pencil will not be remarked.
2. This test consists of eight (8) numbered pages.
3. You are required to answer all questions on this test.
TOTAL: ________ / _______ = ________%
Part A. True or False. Circle "T" for True, "F" for false. 1 mark each.
T F 1. The "E-site" in the ribosome is an abbreviation for the
"encoding-site".
T F 2. Peptide bond formation happens in the ribosome when an
amino acid (alone or as part of an already elongating chain
of amino acids) is detached from the tRNA in the P-site and
bonded to the amino acid already bound to the tRNA in the
A-site.
T F 3. The nuclear pore complex has a component called the
cytoplasmic ring as well as another component called the
nuclear ring.
T F 4. Telomeres are important structures in the genomes of
bacteria.
T F 5. The sequence 5'-CAAAAGGAAAAC-3' is an example of a
palindromic DNA sequence.
T F 6. DNA-binding proteins that homo-dimerize often bind to
palindromic DNA sequences.
T F 7. The exosome is primarily a 3'-to-5' exoribonuclease.
T F 8. All DNA-binding proteins, including e.g. the glucocorticoid
receptor and histone H4, are sequence-specific, meaning
that they bind to a particular sequence within DNA.
This study source was downloaded by 100000899606070 from CourseHero.com on 10-07-2025 01:14:41 GMT -05:00
https://www.coursehero.com/file/11247700/Midterm-2-Solutions-DNA-Replication-Cell-Cycle-Gene-Expression/
, Test 2 BIOTECH 2M03 - Molecular Biology page 2 of 8
T F 9. Eukaryotic cells ensure that regions of DNA from one
chromosome are kept completely separate from regions of
DNA from any other chromosome within the nucleus.
Part B. Multiple Choice. Circle the letter of the one correct answer.
1 mark each.
10 . Which of the following is not correct regarding the initiation of translation
as it most commonly happens in bacteria?
a. The small ribosomal subunit binds the mRNA before the large
ribosomal subunit.
b. The first aminoacyl-tRNA is initially positioned in the A-site of the
ribosome.
c. The anticodon of the first aminoacyl-tRNA brought into the
ribosome has the sequence 5'-CAU-3'.
d. The first aminoacyl-tRNA brought into the ribosome is attached to
the modified amino acid formylmethionine.
e. IF2 is a GTP-binding protein required to bring the first aminoacyl-
tRNA into the ribosome.
11 . A stretch of amino acids in a protein that targets that protein for transport
into the nucleus is known as a(n)
a. chromosomal localizer
b. nucleus targeting region
c. cytoplasmic-nuclear transporter
d. nucleo-cytoplasmic transporter
e. nuclear transport complex
f. nuclear localization signal
12 . Which of the following is not considered to be an important general
mechanism for regulating gene expression (or at least not nearly as
important as the other two)?
a. control of replication (i.e. making DNA from DNA)
b. control of transcription (i.e. making RNA from DNA)
c. control of translation (i.e. making protein from RNA)
This study source was downloaded by 100000899606070 from CourseHero.com on 10-07-2025 01:14:41 GMT -05:00
https://www.coursehero.com/file/11247700/Midterm-2-Solutions-DNA-Replication-Cell-Cycle-Gene-Expression/
TEST 2 (version 1) - Nov 12, 2012 - Answers
Name: _______________________________________
MacID:
Instructions:
1. Tests written in pencil will not be remarked.
2. This test consists of eight (8) numbered pages.
3. You are required to answer all questions on this test.
TOTAL: ________ / _______ = ________%
Part A. True or False. Circle "T" for True, "F" for false. 1 mark each.
T F 1. The "E-site" in the ribosome is an abbreviation for the
"encoding-site".
T F 2. Peptide bond formation happens in the ribosome when an
amino acid (alone or as part of an already elongating chain
of amino acids) is detached from the tRNA in the P-site and
bonded to the amino acid already bound to the tRNA in the
A-site.
T F 3. The nuclear pore complex has a component called the
cytoplasmic ring as well as another component called the
nuclear ring.
T F 4. Telomeres are important structures in the genomes of
bacteria.
T F 5. The sequence 5'-CAAAAGGAAAAC-3' is an example of a
palindromic DNA sequence.
T F 6. DNA-binding proteins that homo-dimerize often bind to
palindromic DNA sequences.
T F 7. The exosome is primarily a 3'-to-5' exoribonuclease.
T F 8. All DNA-binding proteins, including e.g. the glucocorticoid
receptor and histone H4, are sequence-specific, meaning
that they bind to a particular sequence within DNA.
This study source was downloaded by 100000899606070 from CourseHero.com on 10-07-2025 01:14:41 GMT -05:00
https://www.coursehero.com/file/11247700/Midterm-2-Solutions-DNA-Replication-Cell-Cycle-Gene-Expression/
, Test 2 BIOTECH 2M03 - Molecular Biology page 2 of 8
T F 9. Eukaryotic cells ensure that regions of DNA from one
chromosome are kept completely separate from regions of
DNA from any other chromosome within the nucleus.
Part B. Multiple Choice. Circle the letter of the one correct answer.
1 mark each.
10 . Which of the following is not correct regarding the initiation of translation
as it most commonly happens in bacteria?
a. The small ribosomal subunit binds the mRNA before the large
ribosomal subunit.
b. The first aminoacyl-tRNA is initially positioned in the A-site of the
ribosome.
c. The anticodon of the first aminoacyl-tRNA brought into the
ribosome has the sequence 5'-CAU-3'.
d. The first aminoacyl-tRNA brought into the ribosome is attached to
the modified amino acid formylmethionine.
e. IF2 is a GTP-binding protein required to bring the first aminoacyl-
tRNA into the ribosome.
11 . A stretch of amino acids in a protein that targets that protein for transport
into the nucleus is known as a(n)
a. chromosomal localizer
b. nucleus targeting region
c. cytoplasmic-nuclear transporter
d. nucleo-cytoplasmic transporter
e. nuclear transport complex
f. nuclear localization signal
12 . Which of the following is not considered to be an important general
mechanism for regulating gene expression (or at least not nearly as
important as the other two)?
a. control of replication (i.e. making DNA from DNA)
b. control of transcription (i.e. making RNA from DNA)
c. control of translation (i.e. making protein from RNA)
This study source was downloaded by 100000899606070 from CourseHero.com on 10-07-2025 01:14:41 GMT -05:00
https://www.coursehero.com/file/11247700/Midterm-2-Solutions-DNA-Replication-Cell-Cycle-Gene-Expression/
