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Cytogenetics ASCP TEST Questions With 100% Correct Answers.

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Day 1 FISH - CORRECT ANSWER-1. Denatured in 2xSSC for 2 mins 2. EtOH bath for 2 mins each (70%,80%,90%) 3. Dry very well ALL FISH B-CELL - CORRECT ANSWER--CEP 4,10,17 -t(9;22) BCR/ABL DUAL COLOR/DUAL FUSION -t(12;21) ETV6/RUNX1 DUAL COLOR SINGLE FUSION -11q23 MLL (rearrangement/deletion break apart) ALL FISH T-CELL - CORRECT ANSWER--t(9;22) BCR/ABL AML FISH - CORRECT ANSWER--t(8;21) -INV(16) OR t(16;16) -11q23 MLL rearrangement/deletion 45, X - CORRECT ANSWER-Turner syndrome, webbing of the neck, short stature, amnorrhea 47, XXY - CORRECT ANSWER-Klinefelter syndrome, tall stature, sterility, gynecomastia 47, XX, +13 - CORRECT ANSWER-Patau syndrome, severe mental retardation, and physical deformities 47, XX,+18 - CORRECT ANSWER-Edwards syndrome del(4)(p16) - CORRECT ANSWER-Wolf Hirschhorn del(5)(p15) - CORRECT ANSWER-Cri-du-Chat del(13)(q14) - CORRECT ANSWER-retinoblastoma dup(22)(pterq-11) - CORRECT ANSWER-cat's eye syndrome TEL/AML - CORRECT ANSWER--t(12;21) -ETV6-12 -RUNX1-21 M2 - CORRECT ANSWER--t(8;21)(q22;q22) ETO/RUNx1 -RUNx1T1-8q21.3 -RUNx1-21q22

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Cytogenetics ASCP TEST
Day 1 FISH - CORRECT ANSWER-1. Denatured in 2xSSC for 2 mins
2. EtOH bath for 2 mins each (70%,80%,90%)
3. Dry very well

ALL FISH B-CELL - CORRECT ANSWER--CEP 4,10,17
-t(9;22) BCR/ABL DUAL COLOR/DUAL FUSION
-t(12;21) ETV6/RUNX1 DUAL COLOR SINGLE FUSION
-11q23 MLL (rearrangement/deletion break apart)

ALL FISH T-CELL - CORRECT ANSWER--t(9;22) BCR/ABL

AML FISH - CORRECT ANSWER--t(8;21)
-INV(16) OR t(16;16)
-11q23 MLL rearrangement/deletion

45, X - CORRECT ANSWER-Turner syndrome, webbing of the neck, short stature,
amnorrhea

47, XXY - CORRECT ANSWER-Klinefelter syndrome, tall stature, sterility,
gynecomastia

47, XX, +13 - CORRECT ANSWER-Patau syndrome, severe mental retardation, and
physical deformities

47, XX,+18 - CORRECT ANSWER-Edwards syndrome

del(4)(p16) - CORRECT ANSWER-Wolf Hirschhorn

del(5)(p15) - CORRECT ANSWER-Cri-du-Chat

del(13)(q14) - CORRECT ANSWER-retinoblastoma

dup(22)(pterq-11) - CORRECT ANSWER-cat's eye syndrome

TEL/AML - CORRECT ANSWER--t(12;21)
-ETV6-12
-RUNX1-21

M2 - CORRECT ANSWER--t(8;21)(q22;q22)
ETO/RUNx1
-RUNx1T1-8q21.3
-RUNx1-21q22

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