OCR A-Level Biology Foundations In Biology Exam - Study Guide-With 100% verified
answers
Staining - (answer)Allows specimen to become more visible to see certain (named) organelles and
improve contrast
methylene blue - (answer)All purpose
Eosin - (answer)cytoplasm, pink
Acetic orcein - (answer)stains DNA dark red
Sudan - (answer)lipids
Iodine - (answer)plasma membrane
light microscope: magnification and resolution - (answer)1500x and 200nm
Transmission electron microscope - (answer)2,000,000 x magnification
0.5 nm resolution
2D image
Electrons go through specimen
Scanning electron microscope - (answer)200,000 x magnification
3-10nm resolution
3D image
Electrons bounce off specimen
Laser scanning confocal microscopes - (answer)Sample treated with fluorescent dye, laser focuses on
this allowing dye to be seen. High resolution
, OCR A-Level Biology Foundations In Biology Exam - Study Guide-With 100% verified
answers
used in diagnostics e.g. eye problem's
Preparation of sample for magnification - (answer)Stain it
ensure it is thin enough so light can pass through
place on microscope slide at one end
use second slide to spread sample
place cover slips on at an angle
Scientific drawings - (answer)Over 1/2 of page
clear, correct lines
correct proportions
labels with ruled lines, not crossing over
annotations
scale
title
flagella - (answer)9,2 microtubule arrangement
rotate to allow cell to move
Interrelationship of organelles in making proteins - (answer)Uncles site of transcription, makes mRNA,
leaves via pores
ribosomes: translation occurs
vesicles to RER: folded into tertiary structure
vesicles to Golgi apparatus: modified by addition of sugars
vesciles to plasma membrane: exocytosis occurs
mitochondria: ATP for exocytosis
answers
Staining - (answer)Allows specimen to become more visible to see certain (named) organelles and
improve contrast
methylene blue - (answer)All purpose
Eosin - (answer)cytoplasm, pink
Acetic orcein - (answer)stains DNA dark red
Sudan - (answer)lipids
Iodine - (answer)plasma membrane
light microscope: magnification and resolution - (answer)1500x and 200nm
Transmission electron microscope - (answer)2,000,000 x magnification
0.5 nm resolution
2D image
Electrons go through specimen
Scanning electron microscope - (answer)200,000 x magnification
3-10nm resolution
3D image
Electrons bounce off specimen
Laser scanning confocal microscopes - (answer)Sample treated with fluorescent dye, laser focuses on
this allowing dye to be seen. High resolution
, OCR A-Level Biology Foundations In Biology Exam - Study Guide-With 100% verified
answers
used in diagnostics e.g. eye problem's
Preparation of sample for magnification - (answer)Stain it
ensure it is thin enough so light can pass through
place on microscope slide at one end
use second slide to spread sample
place cover slips on at an angle
Scientific drawings - (answer)Over 1/2 of page
clear, correct lines
correct proportions
labels with ruled lines, not crossing over
annotations
scale
title
flagella - (answer)9,2 microtubule arrangement
rotate to allow cell to move
Interrelationship of organelles in making proteins - (answer)Uncles site of transcription, makes mRNA,
leaves via pores
ribosomes: translation occurs
vesicles to RER: folded into tertiary structure
vesicles to Golgi apparatus: modified by addition of sugars
vesciles to plasma membrane: exocytosis occurs
mitochondria: ATP for exocytosis
