ASCP MLT CHEMISTRY EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT DETAILED
ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids? - (answer) nitrogen
What proteins is soluble in water? - (answer) albumin
How are amino acids joined together to form a chain like structure? - (answer) peptide bonding
Proteins may become denatured when subjected to? - (answer) mechanical agitation, heat, chemical
treatments
What does denaturation of proteins refer to? - (answer) alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - (answer) cystinuria
How many immunoglobulin classes are currently recognized? - (answer) five
which reagent is employed in the serum protein determination? - (answer) biuret
What should hemolyzed serum not be used for total protein analysis? - (answer) hemoglobin is
absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - (answer) is relatively free from interference by lipids and
hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - (answer) there
is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - (answer) albumin
,ASCP MLT CHEMISTRY EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT DETAILED
ANSWERS
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen in
which disease state? - (answer) acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin, and 45%
gamma-globulin, then what condition may exist in the patient? - (answer) monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - (answer) carbohydrates
Name some glycoproteins? - (answer) ceruloplasmin, follicle-stimulating hormone, cryoglobulin
What applies to cryoglobulins? - (answer) they are temperature-sensitive proteins
What is true of albumen? - (answer) it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - (answer) Down Syndrome
What protein transports iron? - (answer) transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - (answer) blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - (answer) ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxime method? -
(answer) The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - (answer) elevated
blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - (answer) Jaffe
, ASCP MLT CHEMISTRY EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT DETAILED
ANSWERS
The measurement of creatinine is based on the formation of a yellow-red color and which reagent? -
(answer) titan yellow
The creatinine clearance test is based on what assumption? - (answer) Creatinine passes into the
ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? - (answer)
creatinine
Creatinine clearance formula - (answer) UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum creatinine is 2.0
mg/dL and urine creatinine is 240 mg/dL. What is the clearance, assuming average body surface area? -
(answer) 64 mL/minute
What disease state is associated with an elevation of serum uric acid? - (answer) gout
Which reagent is employed in the alkaline oxidation of uric acid? - (answer) phosphotungistic acid
What does the uricase method for uric acid depend on? - (answer) ultraviolet absorption at 290 nm
before/after treatment with uricase
Which analytes is used as a prognostic indicator for liver failure? - (answer) ammonia
Which disaccharide is composed of two glucose molecules? - (answer) maltose
ANSWERS
Which element in proteins differentiates substance from carbohydrates and lipids? - (answer) nitrogen
What proteins is soluble in water? - (answer) albumin
How are amino acids joined together to form a chain like structure? - (answer) peptide bonding
Proteins may become denatured when subjected to? - (answer) mechanical agitation, heat, chemical
treatments
What does denaturation of proteins refer to? - (answer) alteration in tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - (answer) cystinuria
How many immunoglobulin classes are currently recognized? - (answer) five
which reagent is employed in the serum protein determination? - (answer) biuret
What should hemolyzed serum not be used for total protein analysis? - (answer) hemoglobin is
absorbed at the same wavelength as protein in the reagent
What is NOT true of the biuret reaction? - (answer) is relatively free from interference by lipids and
hemoglobin
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - (answer) there
is less interference from pigment (s)
What is the most anodic protein on electrophoresis at pH 8.6? - (answer) albumin
,ASCP MLT CHEMISTRY EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT DETAILED
ANSWERS
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad gamma is seen in
which disease state? - (answer) acute inflammation
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4-10% beta-globulin, and 45%
gamma-globulin, then what condition may exist in the patient? - (answer) monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - (answer) carbohydrates
Name some glycoproteins? - (answer) ceruloplasmin, follicle-stimulating hormone, cryoglobulin
What applies to cryoglobulins? - (answer) they are temperature-sensitive proteins
What is true of albumen? - (answer) it is produced in the liver and it is water soluble
Decreased alpha-fetoprotein values are associated with what condition? - (answer) Down Syndrome
What protein transports iron? - (answer) transferrin
In acute renal failure, what non protein nitrogen rises the fastest? - (answer) blood urea nitrogen
By the urease method, urea is enzymatically converted to which end product? - (answer) ammonia
Why can untreated urine be used for the determination of urea by the diacetyl monoxime method? -
(answer) The method is not measureing ammonia
An elevated creatinine value is most likely to be accompanied by what values? - (answer) elevated
blood urea nitrogen, 10X creatinine value
What is the classical method for creatinine reaction? - (answer) Jaffe
, ASCP MLT CHEMISTRY EXAM LATEST 2023-2024 ACTUAL EXAM 200 QUESTIONS AND CORRECT DETAILED
ANSWERS
The measurement of creatinine is based on the formation of a yellow-red color and which reagent? -
(answer) titan yellow
The creatinine clearance test is based on what assumption? - (answer) Creatinine passes into the
ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate? - (answer)
creatinine
Creatinine clearance formula - (answer) UV/P
or UV/P (1.73/correction factor)
1440 mls/min
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum creatinine is 2.0
mg/dL and urine creatinine is 240 mg/dL. What is the clearance, assuming average body surface area? -
(answer) 64 mL/minute
What disease state is associated with an elevation of serum uric acid? - (answer) gout
Which reagent is employed in the alkaline oxidation of uric acid? - (answer) phosphotungistic acid
What does the uricase method for uric acid depend on? - (answer) ultraviolet absorption at 290 nm
before/after treatment with uricase
Which analytes is used as a prognostic indicator for liver failure? - (answer) ammonia
Which disaccharide is composed of two glucose molecules? - (answer) maltose
