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National Phlebotomy Certification Exam 2025

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National Phlebotomy Certification Exam 2025 An individual who has little resistance to an infectious microbe is referred to as a susceptible what? - Correct Ans-Host Which of the following is an example of a work practice control that reduces risk of exposure to bloodborne pathogens? - Correct Ans-Wearing gloves to draw blood This ion is essential to the coagulation process. - Correct Ans-Calcium QC protocols prohibit use of outdated evacuated tubes because why? - Correct Ans Tubes my not fill completely, changing additive-to-sample ratios. Molecular genetic testing requires what? - Correct Ans-Specimens to be collected in sterile EDTA. What is the purpose of an antiglycolytic agent? - Correct Ans-Preserve glucose Which on of the following tubes is filled first when multiple tubes are filled using an evacuated tube system? - Correct Ans-Blood culture (SPS) tube What is the recommended disinfectant for blood culture sites in infants 2 months and older? - Correct Ans-Chlorhexidine gluconate Latent fibrin formation in serum can result from what? - Correct Ans-Incomplete clotting when centrifuged In which of the following areas does capillary specimen collection differ from routine venipuncture when collecting a BUN and CBC? - Correct Ans-Order of draw This is an example of a preanalytical error made at the time of collection. - Correct Ans Failing to mix tubes What constitutes a positive modified Allen test? - Correct Ans-The hand color returns to normal in 15 seconds. According to CLSI, which tubes should be placed upright as soon as they are mixed? - Correct Ans-Nonanticoagulant gel tubes Reference values are higher for capillary specimens with what test? - Correct Ans Glucose Phlebotomy Phlebotomy According to CLSI, the maximum time limit for separating serum or plasma from cells is how long? - Correct Ans-2.0 hours from time of collection All of the following are a reason for transporting tubes with the stoppers up EXCEPT which of the following? - Correct Ans-Maintains the sterility of the sample. The liquid portion of a clotted specimen is called what? - Correct Ans-Serum The most common reason for glucose monitoring through POCT is to what? - Correct Ans-Monitor glucose levels for diabetic care. The AMT, ACA, and ASCP are agencies that do what? - Correct Ans-Certify laboratory professionals A quadriplegic patient can do what of the following? - Correct Ans-Can feel pain if there is no sensory damage. The standard of care used in phlebotomy malpractice cases is often based on guidelines from what organization? - Correct Ans-CLSI - Clinical and Laboratory Standards Institute In collecting blood cultures, one of the most frequent errors made is what? - Correct Ans-Improper cleansing of the collection site Which type of specimen is typically used for routine urinalysis? - Correct Ans-Random A positive FIT test indicates what? - Correct Ans-Bleeding in the colon or rectum Accessioning a specimen involves what? - Correct Ans-AOTA-Connecting it with the correct individual, giving it a unique identification number, and recording it in the order it was received. A blood specimen collected in nongel tube has been centrifuged. The cells at the bottom are free flowing and can be resuspended if the tube is inverted. The term used to describe the liquid portion on top of the cells is what? - Correct Ans-Plasma The composition of capillary puncture blood more closely resembles what? - Correct Ans-Arterial Blood A phlebotomist arrives to collect a 2-hour post-prandial glucose specimen on an inpatient and discovers that 2 hours have not elapsed since the patient's meal. What should the phlebotomist do? - Correct Ans-Ask the patient's nurse to verify the correct time to draw the specimen. Which of the following is referred to as a delivering chamber of the heart? - Correct Ans Left ventricle Phlebotomy Phlebotomy According to laboratory policy, specimens collected need to yield 2.5 times the amount of sample needed to perform the test. If a chemistry test requires 0.5mL of serum, which of the following tubes is the smallest that can be used? - Correct Ans-3-mL red top The main difference between serum and plasma is what? - Correct Ans-Plasma has fibrinogen, serum does not. Which of the following represents proper collection and handling of newborn screening specimens? - Correct Ans-Use one free-flowing drop to fill both sides; air-dry flat and elevated. Which of the following is a safe area for infant heel puncture? - Correct Ans-Medial plantar surface Generally, the minimum force and time required to result in good separation of serum or plasma from the cells is what? - Correct Ans-1,000 g for 10 minutes All of the following statements concerning an employee bloodborne pathogen exposure incident are true EXCEPT what? - Correct Ans-The source patient must submit to HIV and HBV testing within 48 hours. Which additive prevents coagulation by binding calcium? - Correct Ans-Potassium EDTA A prothrombin specimen must be collected from a patient with IVs in both arms. The best place to collect the specimen is from where? - Correct Ans-Below on of the IVs Biological Substance Category B regulations must be followed when shipping laboratory specimens for diagnostic purposes by public transportation. These regulations require the specimens to be what? - Correct Ans-Triple packaged in a watertight primary container. Which of the following microcollection containers should be filled first if collected by capillary puncture? - Correct Ans-Lavender top Which of the following specimens is negatively affected by chilling? - Correct Ans Potassium The CAP requires QC for many waived tests to be performed when? - Correct Ans When test manufacturer specifies. A technologist asks the phlebotomist to collect 5cc of whole blood for a special test. What volume tube should be used? - Correct Ans-5 mL Phlebotomy Phlebotomy Transporting tubes with the stopper up has nothing to do with what? - Correct Ans DOES NOT MAINTAIN THE STERILITY OF THE SAMPLE! The first tube of cerebrospinal fluid (CSF) collected is typically used for what? - Correct Ans-Chemistry studies The manufacturer must supply an SDS for which one? - Correct Ans-Isopropyl and methyl alcohol. The abbreviation for the federal agency that instituted and enforces regulations requiring the labeling of hazardous materials is what? - Correct Ans-OSHA What is NAACLS? - Correct Ans-National Accrediting Agency for Clinical Laboratory Sciences - developed the standardized educational curriculum with a recognized body of knowledge, skills, and standards of practice for the phlebotomy profession. What is CLIA? - Correct Ans-Clinical Laboratory Improvement Amendments TJC - Correct Ans-The Joint Commission - independent, not for profit organization charged with, among other things, establishing standards for the operation of hospitals and other health related facilities and services. NPSG - Correct Ans-National Patient Safety Goals CAP - Correct Ans-College of American Pathologists - not for profit organization for professionals in the field of laboratory medicine. Offers proficiency testing and a continuous form of laboratory inspection by a team made up of pathologists and laboratory managers. CLIAC - Correct Ans-Clinical Laboratory Improvement Advisory Committee - to provide technical and scientific advice and guidance to the appropriate people in CMS. NAACLS - Correct Ans-National Accrediting Agency for Clinical Laboratory Sciences - an authority on quality clinical laboratory education. CLSI - Correct Ans-Clinical and Laboratory Standard Institute - organization that develops clinical and laboratory practices and promotes their uses worldwide. NHSN - Correct Ans-National Healthcare Safety Network - provides data on healthcare associated infections. HICPAC - Correct Ans-Healthcare Infection Control Practices Advisory Committee - provides the CDC with advice and guidance regarding the practice of infection control and prevention in healthcare settings. Phlebotomy Phlebotomy CDC - Correct Ans-Centers for Disease Control - investigates and controls various diseases. NIOSH - Correct Ans-National Institute for Occupational Safety and Health - part of the CDC, responsible for conducting research and making recommendations for the prevention of work related illness and injury. OSHA - Correct Ans-Occupational Safety and Health Administration - mandates and enforces safe working conditions for employees. HCS - Correct Ans-Hazard Communication Standard - HazCom - right to know. First tube Culture - Cinnamon - Correct Ans-Yellow - Microbiology - Blood Culture - SPS Additive - Bacteremia or septicemia 2 bottles - Anaerobic and aerobic (butterfly needle-collect aerobi

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Phlebotomy



National Phlebotomy Certification
Exam 2025
An individual who has little resistance to an infectious microbe is referred to as a
susceptible what? - Correct Ans-Host

Which of the following is an example of a work practice control that reduces risk of
exposure to bloodborne pathogens? - Correct Ans-Wearing gloves to draw blood

This ion is essential to the coagulation process. - Correct Ans-Calcium

QC protocols prohibit use of outdated evacuated tubes because why? - Correct Ans-
Tubes my not fill completely, changing additive-to-sample ratios.

Molecular genetic testing requires what? - Correct Ans-Specimens to be collected in
sterile EDTA.

What is the purpose of an antiglycolytic agent? - Correct Ans-Preserve glucose

Which on of the following tubes is filled first when multiple tubes are filled using an
evacuated tube system? - Correct Ans-Blood culture (SPS) tube

What is the recommended disinfectant for blood culture sites in infants 2 months and
older? - Correct Ans-Chlorhexidine gluconate

Latent fibrin formation in serum can result from what? - Correct Ans-Incomplete clotting
when centrifuged

In which of the following areas does capillary specimen collection differ from routine
venipuncture when collecting a BUN and CBC? - Correct Ans-Order of draw

This is an example of a preanalytical error made at the time of collection. - Correct Ans-
Failing to mix tubes

What constitutes a positive modified Allen test? - Correct Ans-The hand color returns to
normal in 15 seconds.

According to CLSI, which tubes should be placed upright as soon as they are mixed? -
Correct Ans-Nonanticoagulant gel tubes

Reference values are higher for capillary specimens with what test? - Correct Ans-
Glucose



Phlebotomy

, Phlebotomy


According to CLSI, the maximum time limit for separating serum or plasma from cells is
how long? - Correct Ans-2.0 hours from time of collection

All of the following are a reason for transporting tubes with the stoppers up EXCEPT
which of the following? - Correct Ans-Maintains the sterility of the sample.

The liquid portion of a clotted specimen is called what? - Correct Ans-Serum

The most common reason for glucose monitoring through POCT is to what? - Correct
Ans-Monitor glucose levels for diabetic care.

The AMT, ACA, and ASCP are agencies that do what? - Correct Ans-Certify laboratory
professionals

A quadriplegic patient can do what of the following? - Correct Ans-Can feel pain if there
is no sensory damage.

The standard of care used in phlebotomy malpractice cases is often based on
guidelines from what organization? - Correct Ans-CLSI - Clinical and Laboratory
Standards Institute

In collecting blood cultures, one of the most frequent errors made is what? - Correct
Ans-Improper cleansing of the collection site

Which type of specimen is typically used for routine urinalysis? - Correct Ans-Random

A positive FIT test indicates what? - Correct Ans-Bleeding in the colon or rectum

Accessioning a specimen involves what? - Correct Ans-AOTA-Connecting it with the
correct individual, giving it a unique identification number, and recording it in the order it
was received.

A blood specimen collected in nongel tube has been centrifuged. The cells at the
bottom are free flowing and can be resuspended if the tube is inverted. The term used
to describe the liquid portion on top of the cells is what? - Correct Ans-Plasma

The composition of capillary puncture blood more closely resembles what? - Correct
Ans-Arterial Blood

A phlebotomist arrives to collect a 2-hour post-prandial glucose specimen on an
inpatient and discovers that 2 hours have not elapsed since the patient's meal. What
should the phlebotomist do? - Correct Ans-Ask the patient's nurse to verify the correct
time to draw the specimen.

Which of the following is referred to as a delivering chamber of the heart? - Correct Ans-
Left ventricle

Phlebotomy

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