ASCP HEMATOLOGY REVIEW
QUESTIONS & ANSWERS(RATED
A+)
Name the locations of active marrow in adults: - ANSWERsternum, skull, ribs, pelvis,
vertebrae
Where does hematopoiesis take place during the first 2 months of gestation? -
ANSWERyolk sac
Where does the majority of hematopoiesis take place during the 2nd through 7th
month of gestation? - ANSWERspleen
When does the bone marrow take over hematopoiesis? - ANSWERafter birth
What are the stages in order of the erythrocyte lineage? - ANSWER(1)rubriblast,
(2)prorubricyte, (3)rubricyte, (4)metarubricyte, (5)reticulocyte, (6)erytrocyte
What are the stages in order of the granulocyte (neutrophil, eosinophil, basophil)
lineage? - ANSWER(1)myeloblast, (2)promyelocyte, (3)myelocyte,
(4)metamyelocyte, (5)band, (6)segmented (7)eos/baso
What are the stages in order of the macrophage lineage? - ANSWER(1)monoblast,
(2)promonocyte, (3)monocyte, (4)macrophage
True or False: Methemoglobin can bind O2. - ANSWERfalse
What causes an increase in carboxyhemoglobin? - ANSWERsmoking, carbon
monoxide poisoning
What color is carboxyhemoglobin? - ANSWERcherry red
What does the hematocrit measure? - ANSWERpacked cell volume in a percentage
What are sources of error seen in using the microhematocrit method to measure the
hematocrit? - ANSWERfailure to seal tube adequately, incorrect reading due to
uneven clay plug, inappropriate centrifuge specs, excess EDTA resulting in RBC
shrinkage
How is the hematocrit measured using automated methods? - ANSWERcalculated
from MCV and RBC
What are the stages in order of the platelet lineage? - ANSWER(1)megakaryoblast,
(2)promegakaryocyte, (3)megakaryocyte, (4)platelet
, What are the stages in order of the lymphocyte lineage? - ANSWER(1)lymphoblast,
(2)prolymphocyte, (3)lymphocyte
Which growth factors stimulate the erythrocyte lineage? - ANSWERGM-CSF, EPO,
IL-3
Which growth factors stimulate the granulocyte lineages? - ANSWERGM-CSF, IL-3
Which growth factors stimulate the platelet lineage? - ANSWERGM-CSF, IL-3, TPO
Which growth factors stimulate the lymphocyte lineage? - ANSWERIL-2, IL-4, IL-6,
IL-7
Which protein functions in the transport of Fe? - ANSWERtransferrin
What is the major storage form of Fe? - ANSWERferritin
What is the long-term (H20) insoluble Fe storage form called? -
ANSWERhemosiderin
Excess Iron is stored where? What does this cause? - ANSWERtissues and body
organs, hemosiderosis, hemochromatosis
Porphyrias are attributed to? - ANSWERexcessive formation of porphrins due to
blockages in any enzymatic step in the heme synthesis pathway.
Red or port wine colored urine can be seen in which condition related to the build up
of heme precursors? - ANSWERporphyria
Neuropsychiatirc symptoms are seen when a build up of which precursors occurs? -
ANSWERearly precursors (Delta-aminolevulinic acid/Porphobilinogen)
Cutaneous symptoms such as photo-sensitivity and facial hair are seen when a build
up of which precursors occurs? - ANSWERlater precursors
(uroporphyrinogen/coproporphyrinogen, protoporphyrin/heme)
Synthesis of heme requires what two components? - ANSWERiron, protoporphyrin
What are the heme precursors in order of synthesis? - ANSWER(1)delta-
aminolevulinic acid, (2)porphobilinogen, (3)uroporphyrinogen,
(4)coproporphyrinogen, (5)protoporphyrin, (6)heme
What type of hemoglobin is seen primarily in newborns? - ANSWERHgb F
What type of hemoglobin is seen primarily in adults? - ANSWERHgb A2
What type of hemoglobin is rarely seen in adults as well as newborns? -
ANSWERHgb A
QUESTIONS & ANSWERS(RATED
A+)
Name the locations of active marrow in adults: - ANSWERsternum, skull, ribs, pelvis,
vertebrae
Where does hematopoiesis take place during the first 2 months of gestation? -
ANSWERyolk sac
Where does the majority of hematopoiesis take place during the 2nd through 7th
month of gestation? - ANSWERspleen
When does the bone marrow take over hematopoiesis? - ANSWERafter birth
What are the stages in order of the erythrocyte lineage? - ANSWER(1)rubriblast,
(2)prorubricyte, (3)rubricyte, (4)metarubricyte, (5)reticulocyte, (6)erytrocyte
What are the stages in order of the granulocyte (neutrophil, eosinophil, basophil)
lineage? - ANSWER(1)myeloblast, (2)promyelocyte, (3)myelocyte,
(4)metamyelocyte, (5)band, (6)segmented (7)eos/baso
What are the stages in order of the macrophage lineage? - ANSWER(1)monoblast,
(2)promonocyte, (3)monocyte, (4)macrophage
True or False: Methemoglobin can bind O2. - ANSWERfalse
What causes an increase in carboxyhemoglobin? - ANSWERsmoking, carbon
monoxide poisoning
What color is carboxyhemoglobin? - ANSWERcherry red
What does the hematocrit measure? - ANSWERpacked cell volume in a percentage
What are sources of error seen in using the microhematocrit method to measure the
hematocrit? - ANSWERfailure to seal tube adequately, incorrect reading due to
uneven clay plug, inappropriate centrifuge specs, excess EDTA resulting in RBC
shrinkage
How is the hematocrit measured using automated methods? - ANSWERcalculated
from MCV and RBC
What are the stages in order of the platelet lineage? - ANSWER(1)megakaryoblast,
(2)promegakaryocyte, (3)megakaryocyte, (4)platelet
, What are the stages in order of the lymphocyte lineage? - ANSWER(1)lymphoblast,
(2)prolymphocyte, (3)lymphocyte
Which growth factors stimulate the erythrocyte lineage? - ANSWERGM-CSF, EPO,
IL-3
Which growth factors stimulate the granulocyte lineages? - ANSWERGM-CSF, IL-3
Which growth factors stimulate the platelet lineage? - ANSWERGM-CSF, IL-3, TPO
Which growth factors stimulate the lymphocyte lineage? - ANSWERIL-2, IL-4, IL-6,
IL-7
Which protein functions in the transport of Fe? - ANSWERtransferrin
What is the major storage form of Fe? - ANSWERferritin
What is the long-term (H20) insoluble Fe storage form called? -
ANSWERhemosiderin
Excess Iron is stored where? What does this cause? - ANSWERtissues and body
organs, hemosiderosis, hemochromatosis
Porphyrias are attributed to? - ANSWERexcessive formation of porphrins due to
blockages in any enzymatic step in the heme synthesis pathway.
Red or port wine colored urine can be seen in which condition related to the build up
of heme precursors? - ANSWERporphyria
Neuropsychiatirc symptoms are seen when a build up of which precursors occurs? -
ANSWERearly precursors (Delta-aminolevulinic acid/Porphobilinogen)
Cutaneous symptoms such as photo-sensitivity and facial hair are seen when a build
up of which precursors occurs? - ANSWERlater precursors
(uroporphyrinogen/coproporphyrinogen, protoporphyrin/heme)
Synthesis of heme requires what two components? - ANSWERiron, protoporphyrin
What are the heme precursors in order of synthesis? - ANSWER(1)delta-
aminolevulinic acid, (2)porphobilinogen, (3)uroporphyrinogen,
(4)coproporphyrinogen, (5)protoporphyrin, (6)heme
What type of hemoglobin is seen primarily in newborns? - ANSWERHgb F
What type of hemoglobin is seen primarily in adults? - ANSWERHgb A2
What type of hemoglobin is rarely seen in adults as well as newborns? -
ANSWERHgb A
