Unit 2 Flashcards
, What is cell fractionation?
QUESTION
Process where cells are
broken up and constituent
organelles are separated out
ANSWER
, What are the conditions for
cell fractionation?
QUESTION
Cold- reduce enzyme activity
that could break down
organelles
Isotonic- prevent bursting or
shrinking of organelles
Buffered- prevent pH
changes that could alter
organelle structure
ANSWER
, What is homogenation?
QUESTION
Cells broken releasing
organelles producing
homogenate that is filtered
to remove unbroken cells
and large debris
ANSWER
, What is cell fractionation?
QUESTION
Process where cells are
broken up and constituent
organelles are separated out
ANSWER
, What are the conditions for
cell fractionation?
QUESTION
Cold- reduce enzyme activity
that could break down
organelles
Isotonic- prevent bursting or
shrinking of organelles
Buffered- prevent pH
changes that could alter
organelle structure
ANSWER
, What is homogenation?
QUESTION
Cells broken releasing
organelles producing
homogenate that is filtered
to remove unbroken cells
and large debris
ANSWER