CHT EXAM PREP EXAM, QUESTIONS WITH
ACCURATE ANswers MULTIPLE CHOICES (2025-
2026 update)
What do ASHI Standards say about running both the optical and fluorescent standards for flow
cytometry?
A. Run each time the instrument is turned on
B. Run any time maintenance has occurred
C. Run any time problems have occurred that could potentially affect instrument function
D. All of the above - ANS>>D. All of the above
The initial recipient workup for a kidney transplant includes all of the following EXCEPT?
A. Establishing the cause of the ESRD (End Stage Renal Disease), if possible
B. Measuring the GFR (glomerular filtration rate)
C. Performing a health examination and blood work
D. Determining if the patient is a U.S. citizen
Determining the psychological and social aspects of the candidate - ANS>>D. Determining if the patient
is a U.S. citizen
Once a candidate has been approved for a transplant, what histocompatibility test(s) is/are carried out?
A. HLA typing
B. Screening for antibodies directed to foreign HLA
C. ABO typing
D. HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO typing - ANS>>D.
HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO typing
,All of the following are true about living donors EXCEPT?
A. Must be over 16 years of age
B. Must be at low risk for any complications associated with transplantation
C. Must be making this decision freely and for altruistic reasons
D. Must not exhibit any behaviors that may increase the risk for disease transmission
E. Must sign an Informed Consent form - ANS>>A. Must be over 16 years of age
All of the following are true about unacceptable antigens EXCEPT?
A. Identified by the presence of antibodies in the transplant candidate directed against foreign HLA
B. Entered into UNET for a transplant candidate
C. Used to identify unacceptable donors
D. Cannot be altered once they are initially entered into UNET
E. Their presence can be altered by vaccination or infection - ANS>>D. Cannot be altered once they are
initially entered into UNET
What group oversees the initial activities related to deceased donors like obtaining family consent for
donation, overseeing the clinical management of the donor, and beginning the organ allocation
procedures?
A. UNOS
B. Organ Procurement Organization (OPO)
C. Candidate's transplant program
D. HLA laboratory
E. Local hospital where the donor is located - ANS>>B. Organ Procurement Organization (OPO)
The virtual crossmatch between the deceased donor and potential recipients considers all of the
following EXCEPT?
A. Unacceptable antigens
B. CREG groups and epitopes
C. Antibodies directed to DRA
D. Possible false positive antibody reactions in patient's medical history
E. Past positive, current negative antibodies - ANS>>C. Antibodies directed to DRA
,A discrepancy between the virtual crossmatch and the physical crossmatch may be caused by which of
the following?
A. Technical problem when performing physical crossmatch
B. Presence of IgM in serum
C. Denatured antigen on single antigen beads used for antibody test
D. Mismatched allele not on panel used for antibody test
E. All of the above - ANS>>E. All of the above
Which of the following are required by ASHI's standards for transplant support?
A. Protocols for patients at high risk for allograft rejection
B. Policy on requirements for the sensitivity and specificity of the test system required to support
clinical transplant protocols
C. Policy to periodically screen serum samples from each transplant patient for antibody to HLA
antigens, including the frequency of screening serum samples
D. Policy for storage and maintenance of relevant transplant samples
E. All of these are required by ASHI standards. - ANS>>E. All of these are required by ASHI standards.
Preformed HLA antibodies in the transplant candidate that are directed against mismatched HLA in a
potential donor can cause?
A. Hyperacute rejection
B. T cell mediated rejection
C. A delay in engraftment of the kidney
D. Chronic rejection
E. All of the above - ANS>>A. Hyperacute rejection
Where in the cell does transcription (transferring information from DNA to mRNA) take place?
A. Nucleus
B. Cytoplasm
C. Endoplasmic reticulum
D. Golgi apparatus
, E. Lysosome - ANS>>A. Nucleus
Which of the following is NOT true about the genetic code?
A. Each codon is 3 letters long
B. There are codons that "tell" the ribosome to terminate synthesis
C. Each amino acid is specified by only a single codon
D. A codon in the mRNA might be GUA, corresponding to GTA in the DNA
E. Ribosomes read the code during protein synthesis - ANS>>C. Each amino acid is specified by only a
single codon
How many hydrogen bonds link the nucleotide bases G and T in double stranded DNA?
A. 4
B. 3
C. 2
D. 1
E. 0 - ANS>>E. 0
Which amino acid can form disulfide bonds?
A. Leucine
B. Cysteine
C. Phenylalanine
D. Valine
E. Glutamic acid - ANS>>B. Cysteine
What is the term used to characterize an amino acid that prefers to be on the interior of a protein, hidde
away from water?
A. Polar
B. Hydrophobic
C. Positively charged
D. Negatively charged
E. Hydrophilic - ANS>>B. Hydrophobic
ACCURATE ANswers MULTIPLE CHOICES (2025-
2026 update)
What do ASHI Standards say about running both the optical and fluorescent standards for flow
cytometry?
A. Run each time the instrument is turned on
B. Run any time maintenance has occurred
C. Run any time problems have occurred that could potentially affect instrument function
D. All of the above - ANS>>D. All of the above
The initial recipient workup for a kidney transplant includes all of the following EXCEPT?
A. Establishing the cause of the ESRD (End Stage Renal Disease), if possible
B. Measuring the GFR (glomerular filtration rate)
C. Performing a health examination and blood work
D. Determining if the patient is a U.S. citizen
Determining the psychological and social aspects of the candidate - ANS>>D. Determining if the patient
is a U.S. citizen
Once a candidate has been approved for a transplant, what histocompatibility test(s) is/are carried out?
A. HLA typing
B. Screening for antibodies directed to foreign HLA
C. ABO typing
D. HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO typing - ANS>>D.
HLA typing and antibody screening HLA typing, HLA antibody screening, and ABO typing
,All of the following are true about living donors EXCEPT?
A. Must be over 16 years of age
B. Must be at low risk for any complications associated with transplantation
C. Must be making this decision freely and for altruistic reasons
D. Must not exhibit any behaviors that may increase the risk for disease transmission
E. Must sign an Informed Consent form - ANS>>A. Must be over 16 years of age
All of the following are true about unacceptable antigens EXCEPT?
A. Identified by the presence of antibodies in the transplant candidate directed against foreign HLA
B. Entered into UNET for a transplant candidate
C. Used to identify unacceptable donors
D. Cannot be altered once they are initially entered into UNET
E. Their presence can be altered by vaccination or infection - ANS>>D. Cannot be altered once they are
initially entered into UNET
What group oversees the initial activities related to deceased donors like obtaining family consent for
donation, overseeing the clinical management of the donor, and beginning the organ allocation
procedures?
A. UNOS
B. Organ Procurement Organization (OPO)
C. Candidate's transplant program
D. HLA laboratory
E. Local hospital where the donor is located - ANS>>B. Organ Procurement Organization (OPO)
The virtual crossmatch between the deceased donor and potential recipients considers all of the
following EXCEPT?
A. Unacceptable antigens
B. CREG groups and epitopes
C. Antibodies directed to DRA
D. Possible false positive antibody reactions in patient's medical history
E. Past positive, current negative antibodies - ANS>>C. Antibodies directed to DRA
,A discrepancy between the virtual crossmatch and the physical crossmatch may be caused by which of
the following?
A. Technical problem when performing physical crossmatch
B. Presence of IgM in serum
C. Denatured antigen on single antigen beads used for antibody test
D. Mismatched allele not on panel used for antibody test
E. All of the above - ANS>>E. All of the above
Which of the following are required by ASHI's standards for transplant support?
A. Protocols for patients at high risk for allograft rejection
B. Policy on requirements for the sensitivity and specificity of the test system required to support
clinical transplant protocols
C. Policy to periodically screen serum samples from each transplant patient for antibody to HLA
antigens, including the frequency of screening serum samples
D. Policy for storage and maintenance of relevant transplant samples
E. All of these are required by ASHI standards. - ANS>>E. All of these are required by ASHI standards.
Preformed HLA antibodies in the transplant candidate that are directed against mismatched HLA in a
potential donor can cause?
A. Hyperacute rejection
B. T cell mediated rejection
C. A delay in engraftment of the kidney
D. Chronic rejection
E. All of the above - ANS>>A. Hyperacute rejection
Where in the cell does transcription (transferring information from DNA to mRNA) take place?
A. Nucleus
B. Cytoplasm
C. Endoplasmic reticulum
D. Golgi apparatus
, E. Lysosome - ANS>>A. Nucleus
Which of the following is NOT true about the genetic code?
A. Each codon is 3 letters long
B. There are codons that "tell" the ribosome to terminate synthesis
C. Each amino acid is specified by only a single codon
D. A codon in the mRNA might be GUA, corresponding to GTA in the DNA
E. Ribosomes read the code during protein synthesis - ANS>>C. Each amino acid is specified by only a
single codon
How many hydrogen bonds link the nucleotide bases G and T in double stranded DNA?
A. 4
B. 3
C. 2
D. 1
E. 0 - ANS>>E. 0
Which amino acid can form disulfide bonds?
A. Leucine
B. Cysteine
C. Phenylalanine
D. Valine
E. Glutamic acid - ANS>>B. Cysteine
What is the term used to characterize an amino acid that prefers to be on the interior of a protein, hidde
away from water?
A. Polar
B. Hydrophobic
C. Positively charged
D. Negatively charged
E. Hydrophilic - ANS>>B. Hydrophobic
