BCH 403 – CUMULATIVE FINAL
PRACTICE EXAM QUESTIONS
WITH CORRECT ANSWERS
Which of the following is NOT considered a central metabolic pathway?
A. Glycolysis
B. Glycogenesis
C. Citric acid cycle
D. Gluconeogenesis
E. Oxidative phosphorylation - Answer-B. Glycogenesis
Which one of the following molecules is the primary energy source in biological
systems?
A. ATP
B. ADP
C. NADH
D. NAPDH
E. NAD+ - Answer-A. ATP
Which of the following is the primary site of regulation of glycolysis?
A. Hexokinase
B. Pyruvate kinase
C. Phosphoglycerate kinase
D. Phosphofructokinase-1
E. Glyceraldehyde-3-phosphate dehydrogenase - Answer-D. Phosphofructokinase-1
NADH is generated in glycolysis by:
A. Hexokinase
B. Pyruvate kinase
C. Phosphoglycerate kinase
D. Phosphofructokinase-1
E. Glyceraldehyde-3-phosphate dehydrogenase - Answer-E. Glyceraldehyde-3-
phosphate dehydrogenase
In humans, the primary site of gluconeogenesis is the:
A. Brain
B. Skeletal muscle
C. Kidney medulla
D. Kidney cortex
E. Liver - Answer-E. Liver
,All of the following can be used as a precursor for gluconeogenesis, except:
A. Lactate
B. Alanine
C. Glutamine
D. Glycerol
E. Lysine - Answer-E. Lysine
During dietary polysaccharide digestion, α(1,4) linkages are cleaved by:
A. Glycogen phosphorylase
B. Glycogen debranching enzyme
C. Phosphoglucomutase
D. α-amylase
E. Maltase - Answer-D. α-amylase
Glucose is the product of :
A. Glycogen phosphorylase
B. Glycogen debranching enzyme
C. Phosphoglucomutase
D. Uridine diphosphate glucose pyrophosphorylase
E. α-amylase - Answer-B. Glycogen debranching enzyme
Which of the following pathways will be turned on when the adenylate energy charge is
low?
A. Glycolysis and gluconeogenesis
B. Glycogenesis and glycogenolysis
C. Glycogenesis and glycolysis
D. Glycogenolysis and glycolysis
E. Glycogenesis and gluconeogenesis - Answer-D. Glycogenolysis and glycolysis
Which one of the following enzymes is not regulated by the presence of glucagon?
A. Fructose-1,6-bisphosphatase
B. Glucokinase
C. Pyruvate kinase
D. Phosphofructokinase-1
E. Phosphoenolpyruvate carboxykinase - Answer-B. Glucokinase
Which one of the following statements is correct regarding the regulation of glucokinase
and hexokinase?
A. Glucokinase is inhibited by high concentrations of glucose-6-phosphate, but
hexokinase is not.
B. Hexokinase is inhibited by high concentrations of glucose-6-phosphate, but
glucokinase is not.
C. Both enzymes are inhibited by high concentrations of glucose-6-phosphate.
D. Glucokinase is inhibited by high concentrations of glucose, but hexokinase is not.
, E. Hexokinase is inhibited by high concentrations of glucose, but glucokinase is not. -
Answer-B. Hexokinase is inhibited by high concentrations of glucose-6-phosphate, but
glucokinase is not.
Which one of the following molecules directly binds and regulates fructose-1,6-
bisphosphate?
A. Insulin
B. Glucagon
C. Fructose-6-phosphate
D. Fructose-1,6-bisphosphate
E. Fructose-2,6-bisphosphate - Answer-E. Fructose-2,6-bisphosphate
Which one of the following statements regarding glucose oxidation is correct?
A. All 6 carbons from glucose are oxidized to CO2 during the TCA cycle
B. Only 2 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle.
C. Only 4 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle
D. 2 carbons are oxidized to CO2 during glycolysis, while 4 carbons are oxidized to
CO2 during the TCA cycle
E. 2 carbons are oxidized to CO2 by the pyruvate dehydrogenase complex, while 4
carbons are oxidized to CO2 during the TCA cycle - Answer-E. 2 carbons are oxidized
to CO2 by the pyruvate dehydrogenase complex, while 4 carbons are oxidized to CO2
during the TCA cycle
Which one of the following TCA cycle enzymes is irreversible and regulated?
A. Citrate synthase
B. Isocitrate dehydrogenase
C. Succinate dehydrogenase
D. α-ketoglutarate dehydrogenase
E. Aconitase - Answer-A. Citrate synthase
Which of the following statements is correct regarding the regulation of pyruvate
dehydrogenase complex?
A. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
B. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
C. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) phosphatase
D. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
E. Despite being a product of pyruvate dehydrogenase (PDH), acetyl-CoA does not
regulate the activity of PDH - Answer-A. Acetyl-CoA inhibits activity of pyruvate
dehydrogenase by activating pyruvate dehydrogenase (PDH) kinase
PRACTICE EXAM QUESTIONS
WITH CORRECT ANSWERS
Which of the following is NOT considered a central metabolic pathway?
A. Glycolysis
B. Glycogenesis
C. Citric acid cycle
D. Gluconeogenesis
E. Oxidative phosphorylation - Answer-B. Glycogenesis
Which one of the following molecules is the primary energy source in biological
systems?
A. ATP
B. ADP
C. NADH
D. NAPDH
E. NAD+ - Answer-A. ATP
Which of the following is the primary site of regulation of glycolysis?
A. Hexokinase
B. Pyruvate kinase
C. Phosphoglycerate kinase
D. Phosphofructokinase-1
E. Glyceraldehyde-3-phosphate dehydrogenase - Answer-D. Phosphofructokinase-1
NADH is generated in glycolysis by:
A. Hexokinase
B. Pyruvate kinase
C. Phosphoglycerate kinase
D. Phosphofructokinase-1
E. Glyceraldehyde-3-phosphate dehydrogenase - Answer-E. Glyceraldehyde-3-
phosphate dehydrogenase
In humans, the primary site of gluconeogenesis is the:
A. Brain
B. Skeletal muscle
C. Kidney medulla
D. Kidney cortex
E. Liver - Answer-E. Liver
,All of the following can be used as a precursor for gluconeogenesis, except:
A. Lactate
B. Alanine
C. Glutamine
D. Glycerol
E. Lysine - Answer-E. Lysine
During dietary polysaccharide digestion, α(1,4) linkages are cleaved by:
A. Glycogen phosphorylase
B. Glycogen debranching enzyme
C. Phosphoglucomutase
D. α-amylase
E. Maltase - Answer-D. α-amylase
Glucose is the product of :
A. Glycogen phosphorylase
B. Glycogen debranching enzyme
C. Phosphoglucomutase
D. Uridine diphosphate glucose pyrophosphorylase
E. α-amylase - Answer-B. Glycogen debranching enzyme
Which of the following pathways will be turned on when the adenylate energy charge is
low?
A. Glycolysis and gluconeogenesis
B. Glycogenesis and glycogenolysis
C. Glycogenesis and glycolysis
D. Glycogenolysis and glycolysis
E. Glycogenesis and gluconeogenesis - Answer-D. Glycogenolysis and glycolysis
Which one of the following enzymes is not regulated by the presence of glucagon?
A. Fructose-1,6-bisphosphatase
B. Glucokinase
C. Pyruvate kinase
D. Phosphofructokinase-1
E. Phosphoenolpyruvate carboxykinase - Answer-B. Glucokinase
Which one of the following statements is correct regarding the regulation of glucokinase
and hexokinase?
A. Glucokinase is inhibited by high concentrations of glucose-6-phosphate, but
hexokinase is not.
B. Hexokinase is inhibited by high concentrations of glucose-6-phosphate, but
glucokinase is not.
C. Both enzymes are inhibited by high concentrations of glucose-6-phosphate.
D. Glucokinase is inhibited by high concentrations of glucose, but hexokinase is not.
, E. Hexokinase is inhibited by high concentrations of glucose, but glucokinase is not. -
Answer-B. Hexokinase is inhibited by high concentrations of glucose-6-phosphate, but
glucokinase is not.
Which one of the following molecules directly binds and regulates fructose-1,6-
bisphosphate?
A. Insulin
B. Glucagon
C. Fructose-6-phosphate
D. Fructose-1,6-bisphosphate
E. Fructose-2,6-bisphosphate - Answer-E. Fructose-2,6-bisphosphate
Which one of the following statements regarding glucose oxidation is correct?
A. All 6 carbons from glucose are oxidized to CO2 during the TCA cycle
B. Only 2 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle.
C. Only 4 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle
D. 2 carbons are oxidized to CO2 during glycolysis, while 4 carbons are oxidized to
CO2 during the TCA cycle
E. 2 carbons are oxidized to CO2 by the pyruvate dehydrogenase complex, while 4
carbons are oxidized to CO2 during the TCA cycle - Answer-E. 2 carbons are oxidized
to CO2 by the pyruvate dehydrogenase complex, while 4 carbons are oxidized to CO2
during the TCA cycle
Which one of the following TCA cycle enzymes is irreversible and regulated?
A. Citrate synthase
B. Isocitrate dehydrogenase
C. Succinate dehydrogenase
D. α-ketoglutarate dehydrogenase
E. Aconitase - Answer-A. Citrate synthase
Which of the following statements is correct regarding the regulation of pyruvate
dehydrogenase complex?
A. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
B. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
C. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) phosphatase
D. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
E. Despite being a product of pyruvate dehydrogenase (PDH), acetyl-CoA does not
regulate the activity of PDH - Answer-A. Acetyl-CoA inhibits activity of pyruvate
dehydrogenase by activating pyruvate dehydrogenase (PDH) kinase
