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Microbiology Lab Final Exam Exams Actual Exam Newest Complete All Questions And Correct Detailed Answers (Verified Answers) |Already Graded A+||Latest Version!!!

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Institution Microbiology Lab
Course Microbiology Lab
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Microbiology Lab Final Exam Exams Actual Exam Newest Complete All Questions And Correct Detailed Answers (Verified Answers) |Already Graded A+||Latest Version!!!

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Microbiology Lab Final Exam Exams Actual Exam
Newest Complete All Questions And Correct Detailed
Answers (Verified Answers) |Already Graded
A+||Latest Version!!!


Give the steps of a PCR - Answer-Heat reaction to 95 C to
denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind


What machine is used to carry out a PCR reaction? -
Answer-thermocycler


How many cycles does a PCR rxn usually go? - Answer-
30


Name the 9 organisms used in the unknown lab - Answer-
Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae

, 2|Page


Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis


Which organisms can ferment lactose? - Answer-E. coli
E. cloacae
E. aerogenes
K. pneumonia


Define caliform bacteria - Answer-rod-shaped, Gram-
negative, non-spore forming, and motile or non-motile
bacteria which can ferment lactose with the production of
acid and gas


Name the selective medias used in class - Answer-Blood
Agar
Bile Esculin
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)

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