BIO 211 Lab Final || All Accurately Answered.
If labs were in person in a traditional lab setting, what type of PPE are we required to wear in
BIO 211 lab? correct answers Lab coat or apron, gloves, either goggles or safety glasses.
The molecular mass of thymidine is 242 g/mol. What is the concentration of thymidine in 1 liter
of a solution containing 363 grams of thymidine? correct answers 1.5 - 363/242
What is the pH of a 0.01 M solution of hydrochloric acid (HCl)? (remember HCl is a strong acid
and therefore dissociates completely in water) correct answers 2
What is the concentration of hydrogen ions [H+] in a solution that has a pH of 3? (write out
using decimals) correct answers 0.001
What is a buffer (i.e. the chemical kind)? correct answers A solution of a weak acid and/or weak
base that absorbs H+ or OH- and thereby minimizes pH change when either acid or base is added
to it. (there are a lot of different ways to describe this, but these are the main points that need to
be made)
State a hypothesis for Lab 2b. correct answers If the hydrochloric acid is added to the solutions
used, they will all turn acidic.
Which of the following is a violation of safety procedure in the laboratory? correct answers
Wearing close-toed shoes and shorts in the lab.
True or false (circle one): Biological solutions buffer pH change correct answers True
In Lab 2b we tested the hypothesis that biological solutions buffer pH changes caused by
addition of acid. The negative control was: correct answers Water
, Increasing sample size, i.e. the number of samples that you measure, usually has the effect of
_________ the standard deviation. correct answers Decreasing
Which lens(es) on a compound microscope magnify the image of a sample? (select all that
apply) correct answers Objective
Ocular
What is the most important characteristic of a lens that determines its optical quality? correct
answers Numerical aperture
The purpose of Köhler illumination in microscopy is to ensure: correct answers Uniformly bright
illumination.
Which of the following is correlated with better image quality? correct answers lower limit of
resolution
The University at Buffalo's Academic Integrity Policy governs all academic activities, and
specific guidelines for BIO 201 are found in the 'About BIO 201 Labs' document. Which of the
following is NOT a violation of academic integrity? correct answers Showing your lab partner
how to read the volume on a micropipettor.
In order to determine the three-dimensional distribution of secretory vesicles in a cell, you could
label the cells with a vesicle-specific fluorophore, and perform optical sectioning using: correct
answers Laser scanning confocal microscopy
When doing microscopy, Köhler illumination is set up by adjusting the focus of the: correct
answers Ocular lenses.
Fluorescence microscopy involves shining light onto a sample (excitation) and viewing the light
that is emitted back from the sample. Explain how light energy is captured and then emitted by
the fluorophore. In your answer, include what is "excited" by the light of excitation. correct
If labs were in person in a traditional lab setting, what type of PPE are we required to wear in
BIO 211 lab? correct answers Lab coat or apron, gloves, either goggles or safety glasses.
The molecular mass of thymidine is 242 g/mol. What is the concentration of thymidine in 1 liter
of a solution containing 363 grams of thymidine? correct answers 1.5 - 363/242
What is the pH of a 0.01 M solution of hydrochloric acid (HCl)? (remember HCl is a strong acid
and therefore dissociates completely in water) correct answers 2
What is the concentration of hydrogen ions [H+] in a solution that has a pH of 3? (write out
using decimals) correct answers 0.001
What is a buffer (i.e. the chemical kind)? correct answers A solution of a weak acid and/or weak
base that absorbs H+ or OH- and thereby minimizes pH change when either acid or base is added
to it. (there are a lot of different ways to describe this, but these are the main points that need to
be made)
State a hypothesis for Lab 2b. correct answers If the hydrochloric acid is added to the solutions
used, they will all turn acidic.
Which of the following is a violation of safety procedure in the laboratory? correct answers
Wearing close-toed shoes and shorts in the lab.
True or false (circle one): Biological solutions buffer pH change correct answers True
In Lab 2b we tested the hypothesis that biological solutions buffer pH changes caused by
addition of acid. The negative control was: correct answers Water
, Increasing sample size, i.e. the number of samples that you measure, usually has the effect of
_________ the standard deviation. correct answers Decreasing
Which lens(es) on a compound microscope magnify the image of a sample? (select all that
apply) correct answers Objective
Ocular
What is the most important characteristic of a lens that determines its optical quality? correct
answers Numerical aperture
The purpose of Köhler illumination in microscopy is to ensure: correct answers Uniformly bright
illumination.
Which of the following is correlated with better image quality? correct answers lower limit of
resolution
The University at Buffalo's Academic Integrity Policy governs all academic activities, and
specific guidelines for BIO 201 are found in the 'About BIO 201 Labs' document. Which of the
following is NOT a violation of academic integrity? correct answers Showing your lab partner
how to read the volume on a micropipettor.
In order to determine the three-dimensional distribution of secretory vesicles in a cell, you could
label the cells with a vesicle-specific fluorophore, and perform optical sectioning using: correct
answers Laser scanning confocal microscopy
When doing microscopy, Köhler illumination is set up by adjusting the focus of the: correct
answers Ocular lenses.
Fluorescence microscopy involves shining light onto a sample (excitation) and viewing the light
that is emitted back from the sample. Explain how light energy is captured and then emitted by
the fluorophore. In your answer, include what is "excited" by the light of excitation. correct
