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BCHM 307 Exam 1 – Purdue University | 2025/2026 | 60+ Verified Q&A with Explanations | Biochemical Molecules, Protein Structure, Enzymes, Lipids, DNA, and Cellular Metabolism

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This in-depth exam resource contains 60+ expertly solved and explained questions and answers from BCHM 307 Exam 1 at Purdue University, fully updated for the 2025/2026 academic year. Ideal for biochemistry majors and pre-medical students, this study guide covers essential foundational topics in molecular biology and biochemical systems. The exam addresses high-yield concepts such as: Classes of biological macromolecules and their polymeric forms Thermodynamics and entropy in biological systems Protein folding, primary to quaternary structure, and the role of chaperones Acid-base catalysis, enzyme active sites, and Michaelis-Menten kinetics DNA/RNA structure, base pairing, GC content, and heat resistance Cellular pH regulation, buffers, and the digestive transition from stomach to intestine Lipid types, bilayer formation, membrane transport, and detergent action Experimental biochemistry (e.g., Hershey-Chase experiment, site-directed mutagenesis) Microscopy of cytoskeletal proteins (myosin, kinesin) and rigor mortis analysis Nutrition-related metabolism questions (e.g., Vitamin A absorption, sorbitol digestion, yogurt vs capsaicin, fiber metabolism) Each question includes the correct answer with clearly written rationales grounded in biochemistry principles. The structure mirrors real exam formats, helping students prepare for both conceptual and applied multiple-choice and short-answer assessments. Best suited for: Undergraduate students in BCHM 307 or equivalent biochemistry courses Pre-med, pre-pharmacy, and biology majors Students at Purdue University and other research-focused institutions Tutors and teaching assistants looking for comprehensive Q&A practice sets MCAT or graduate-level biosciences exam prep This guide serves as an essential companion to lecture notes and textbooks, ensuring mastery of core topics required for biochemistry success. Keywords: biochemistry exam Purdue, BCHM 307 questions 2025, biological macromolecules, protein structure levels, enzyme catalysis, entropy and disorder, DNA base pairs, GC content, Hershey Chase experiment, lipid bilayer properties, myosin vs kinesin movement, vitamin metabolism, cellulose digestion, enzyme kinetics KM

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BCHM 307 Exam 1 Purdue 2025/2026
Exam Questions and Verified Answers |
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Name the four types of small biological molecules. Which three are capable of

forming polymeric structures? What are the names of the polymeric structures that

are formed? - 🧠 ANSWER ✔✔4 types of biological molecules: amino acids,

nucleotides, monosaccharides, lipids

3 capable of forming polymeric strucures: amino acids, monosccharides, and

nucleotides

Names of polymeric structures formed: proteins, carbohydrates, nucleic acids

A healthy diet must include some protein. Assuming you had a way to measure the

amount of each element in a sample of food, which element would you measure in

order to tell whether the food contained protein? - 🧠 ANSWER ✔✔Assuming you

,had a way to measure each element in a food sample, you should measure for the

element nitrogen if determining the amount of protein. Protein is the only

macronutrient that contains nitrogen.

Which has the greater entropy, a polymeric molecule or a mixture of its constituent

monomers? - 🧠 ANSWER ✔✔The polymeric molecule is much more ordered

compared to a mixture of its constituent monomers. This order results in less

entropy. A constituent monomer may have multiple arrangements which results in

greater entropy.

A soccer coach keeps a couple of instant cold packs in her bag in case one of her

players suffers a muscle injury. Instant cold packs are composed of a plastic bag

containing a smaller water bag and solid ammonium nitrate. In order to activate the

cold pack, the bag is kneaded until the smaller water bag breaks, which allows the

released water to dissolve the ammonium nitrate. the equation for the dissolution of

ammonium nitrate in water is shown below. How does the cold pack work. - 🧠

ANSWER ✔✔The positive charge indicates that the dissolution of ammonium

nitrate is an endothermic process. When the ammonium nitrates dissolves in water,

it absorbs heat from its surroundings, making its surroundings cooler, making it

useable is a cold pack for injuries.

, Which compound has a higher boiling point, H2O or H2S? Explain. - 🧠 ANSWER

✔✔Although both have the same geometry and can form H bonds, the H bonds of

water are stronger. The electronegativity between H and O is greater than between

H and S, resulting in a greater difference in partial charges on the atoms in the

water molecule.

Explain why water forms nearly spherical droplets on the surface of a freshly

waxed car. Why doesn't water bead on a clean windshield. - 🧠 ANSWER

✔✔Water is hydrophilic, so when exposed to a hydrophobic surface, water will try

minimize its exposure to that surface, which is why it beads in the shape of a

spherical droplet on a waxed car. However when a water is exposed to a

hydrophilic surface in which it can interact, such as a clean windshield, it will

spread out.

Several hours after a meal, partially digested food leaves the stomach and enters

the small intestine, where pancreatic juice is added. How does the pH of the

partially digested mixture change as it passes from the stomach into the intestine? -

🧠 ANSWER ✔✔The partially digested food has a low pH until the pancreatic

juices are added. The pancreatic juice is nearly alkaline and therefore will increase

the pH of the partially digested food.




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