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PHLEBOTOMY NATIONAL EXAM (NHA)
2025/2026 | ACTUAL REVISED QUESTIONS AND
VERIFIED ANSWERS
Characteristics imperative to a phlebotomist - correct-answer-Dependability,
honesty, integrity, empathy and compassion, professional appearance,
interpersonal skills
Circulatory system function - correct-answer-deliver oxygen, nutrients, hormones,
& enzymes to cells and transport cellular waste (CO2) to organs where they can be
expelled
The two circulations - correct-answer-Pulmonary & Systemic
Pulmonary circulation - correct-answer-carries deoxygenated blood from the right
ventricle to the lungs and returns oxygenated blood from lungs to left atrium
Where does oxygenation take place in the lungs? - correct-answer-alveoli
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Systemic circulation - correct-answer-carries oxygenated blood from left ventricle
throughout the body
Upper chambers of the heart - correct-answer-right and left atria
lower chambers of the heart - correct-answer-right and left ventricle
tricuspid valve - correct-answer-atrioventricular valve, situated between right
atrium and right ventricle
**to remember this valve is on the right side of the heart-- people TRY to be
RIGHT
ORDER OF DRAW - correct-answer-blood culture tubes, light blue, tiger top/red,
green, lavender, gray
Lavender top tube additive - correct-answer-EDTA
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What does EDTA inhibit? - correct-answer-coagulation by binding calcium
How many times do you invert a lavender? - correct-answer-8
Common tests for a lavender - correct-answer-CBC, WBC differential count,
hemoglobin and hematocrit, ESR, sickle cell screening
Light blue top tube additive - correct-answer-sodium citrate
How many times do you invert a light blue? - correct-answer-3-4
Does a light blue tube need to be completely filled? - correct-answer-YES
Common tests for light blue tubes - correct-answer-Prothrombin time (PT), APTT,
PTT, Fibrinogen degradation products (FDP), bleeding time (BT)
PHLEBOTOMY NATIONAL EXAM (NHA)
2025/2026 | ACTUAL REVISED QUESTIONS AND
VERIFIED ANSWERS
Characteristics imperative to a phlebotomist - correct-answer-Dependability,
honesty, integrity, empathy and compassion, professional appearance,
interpersonal skills
Circulatory system function - correct-answer-deliver oxygen, nutrients, hormones,
& enzymes to cells and transport cellular waste (CO2) to organs where they can be
expelled
The two circulations - correct-answer-Pulmonary & Systemic
Pulmonary circulation - correct-answer-carries deoxygenated blood from the right
ventricle to the lungs and returns oxygenated blood from lungs to left atrium
Where does oxygenation take place in the lungs? - correct-answer-alveoli
,2|Page
Systemic circulation - correct-answer-carries oxygenated blood from left ventricle
throughout the body
Upper chambers of the heart - correct-answer-right and left atria
lower chambers of the heart - correct-answer-right and left ventricle
tricuspid valve - correct-answer-atrioventricular valve, situated between right
atrium and right ventricle
**to remember this valve is on the right side of the heart-- people TRY to be
RIGHT
ORDER OF DRAW - correct-answer-blood culture tubes, light blue, tiger top/red,
green, lavender, gray
Lavender top tube additive - correct-answer-EDTA
, 3|Page
What does EDTA inhibit? - correct-answer-coagulation by binding calcium
How many times do you invert a lavender? - correct-answer-8
Common tests for a lavender - correct-answer-CBC, WBC differential count,
hemoglobin and hematocrit, ESR, sickle cell screening
Light blue top tube additive - correct-answer-sodium citrate
How many times do you invert a light blue? - correct-answer-3-4
Does a light blue tube need to be completely filled? - correct-answer-YES
Common tests for light blue tubes - correct-answer-Prothrombin time (PT), APTT,
PTT, Fibrinogen degradation products (FDP), bleeding time (BT)
