Portage Biod 171 module 4 exam
Study online at https://quizlet.com/_csh1t5
T/F: differential media is best suited for
true
distinguishing between 2 bacteria
a researcher is asked to determine which
vial contains e. coli and which contains differential media
salmonella. which media is best?
A fastidious microbe is an organism with
complex growth requirements such that
what are the requirements of a fastidious if absent it will not grow. Enriched medias
microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
alpha hemolysis activity no change
beta hemolysis activity greenish-brown color
gamma hemolysis activity distinct zone of clearing
the process of spreading a bacterial cul-
plating
ture onto a petri dish filled with agar
best agar media for growing staph manitol salt
in EMB agar, what color indicates the
metallic green
presence of e. coli?
In an attempt to detect the presence of it should be plated on sorbitol mac-
the pathogenic strain of E. coli O157:H7, Conkey agar, it is formulated to detect
a researcher spread a culture onto pathogenic e. coli it has sorbitol instead
a MacConkey agar with failed results. of lactose and pathogenic e. coli can fer-
What type of agar should they (correctly) ment there- non pathogenic e. coli, can
try next? Why? ferment on both
would a gram stain be necessary to con-
No, because only gram negative mi-
firm if e. coli was gram positive or nega-
crobes will grow on a macconkey agar
tive?
T/F: Chocolate agar gets its brown color
from cocoa to produce an enriched me- false
dia
columbia cna agar is used to isolate: gram positive media
Blood agar is what type of media? enriched and differential
1/3
Study online at https://quizlet.com/_csh1t5
T/F: differential media is best suited for
true
distinguishing between 2 bacteria
a researcher is asked to determine which
vial contains e. coli and which contains differential media
salmonella. which media is best?
A fastidious microbe is an organism with
complex growth requirements such that
what are the requirements of a fastidious if absent it will not grow. Enriched medias
microbe? thus contain these specific and essential
nutrients required for the growth of a par-
ticular subset of microorganisms.
alpha hemolysis activity no change
beta hemolysis activity greenish-brown color
gamma hemolysis activity distinct zone of clearing
the process of spreading a bacterial cul-
plating
ture onto a petri dish filled with agar
best agar media for growing staph manitol salt
in EMB agar, what color indicates the
metallic green
presence of e. coli?
In an attempt to detect the presence of it should be plated on sorbitol mac-
the pathogenic strain of E. coli O157:H7, Conkey agar, it is formulated to detect
a researcher spread a culture onto pathogenic e. coli it has sorbitol instead
a MacConkey agar with failed results. of lactose and pathogenic e. coli can fer-
What type of agar should they (correctly) ment there- non pathogenic e. coli, can
try next? Why? ferment on both
would a gram stain be necessary to con-
No, because only gram negative mi-
firm if e. coli was gram positive or nega-
crobes will grow on a macconkey agar
tive?
T/F: Chocolate agar gets its brown color
from cocoa to produce an enriched me- false
dia
columbia cna agar is used to isolate: gram positive media
Blood agar is what type of media? enriched and differential
1/3
