BIOC 2580 quiz questions (quiz 1 and 2)
with correct answers
Which ||of ||the ||following ||amino ||acids ||will ||bind ||to ||anion ||exchange ||resin ||at ||pH ||7.0?
a. ||Glu
b. ||Met
c. ||Tyr
d. ||Trp ||- ||CORRECT ||ANSWER✔✔-a. ||Glu
Given ||a ||serine ||and ||two ||ammonia ||molecules, ||hydrogen ||bonding ||exists ||between ||which ||
pairs ||of ||atoms? ||- ||CORRECT ||ANSWER✔✔-the ||H ||on ||the ||OH ||group ||on ||serine ||and ||the ||N: ||
on ||one ||ammonia ||molecule
Given ||the ||molecular ||masses ||of ||the ||following ||elements: ||H: ||1 ||Da, ||C: ||12 ||Da, ||N: ||14 ||Da, ||O:
||16 ||Da, ||which ||element ||is ||the ||most ||abundant ||in ||Aspartate ||at ||pH ||7.0 ||by ||weight?
a. ||oxygen
b. ||hydrogen
c. ||carbon
d. ||nitrogen ||- ||CORRECT ||ANSWER✔✔-a. ||oxygen
Which ||of ||the ||following ||is ||TRUE ||about ||mass ||spectrometry?
1) ||Velocity ||is ||inversely ||proportional ||to ||the ||mass
2) ||The ||detector ||can ||yield ||mass ||measurement ||up ||to ||3 ||significant ||figures
3)It ||can ||be ||used ||to ||quantify ||proteins
4)Samples ||need ||to ||be ||neutral ||- ||CORRECT ||ANSWER✔✔-1) ||Velocity ||is ||inversely ||
proportional ||to ||the ||mass
, How ||can ||you ||determine ||how ||many ||peptide ||bonds ||are ||present ||in ||the ||peptide ||shown ||
below? ||- ||CORRECT ||ANSWER✔✔-count ||the ||number ||of ||amide ||bonds: ||between ||double ||
bonded ||O ||and ||NH ||group
An ||atom ||with ||a ||lone ||pair ||of ||electrons ||can ||use ||it ||in ||different ||ways. ||When ||the ||lone ||pair
||of ||electrons ||is ||shared ||with ||another ||electron ||deficient ||atom ||to ||make ||a ||new ||bond, ||it ||is ||
acting ||as ||a ||______________. ||- ||CORRECT ||ANSWER✔✔-nucleophile
Give ||an ||example ||of ||an ||amino ||acid ||that ||contains ||a ||sulfur ||atom. ||Use ||the ||three-letter-
codes. ||- ||CORRECT ||ANSWER✔✔-Cys ||(or ||Met)
At ||pH ||1, ||which ||of ||the ||following ||is ||the ||predominant ||form ||of ||Lys? ||- ||CORRECT ||
ANSWER✔✔-should ||have ||NH3+ ||on ||the ||left
COOH ||on ||the ||right
CH2 ||(x4)
NH3+ ||on ||the ||bottom
Partition ||or ||thin ||layer ||chromatography ||separates ||proteins ||on ||the ||basis ||of ||polarity ||with ||
polar ||amino ||acids ||being ||retained ||on ||the ||stationary ||phase. ||Rank ||the ||following ||in ||order, ||
with ||least ||mobile ||amino ||acid ||first. ||- ||CORRECT ||ANSWER✔✔-1. ||His
2. ||Asn
3. ||Cys
4. ||Ile
A ||mixture ||of ||proteins ||contains ||Trypsinogen ||(24 ||kDa), ||fumerase ||(49 ||kDa), ||Transferrin ||(80 ||
kDa) ||and ||Aprotinin ||(6.5 ||kDa). ||Rank ||these ||proteins ||based ||on ||the ||order ||of ||their ||elution ||
from ||a ||gel ||filtration ||column. ||(1 ||being ||the ||first ||one ||to ||elute ||and ||4 ||as ||the ||last ||one) ||- ||
CORRECT ||ANSWER✔✔-1. ||transferrin
2. ||fumerase
3. ||trypsinogen
with correct answers
Which ||of ||the ||following ||amino ||acids ||will ||bind ||to ||anion ||exchange ||resin ||at ||pH ||7.0?
a. ||Glu
b. ||Met
c. ||Tyr
d. ||Trp ||- ||CORRECT ||ANSWER✔✔-a. ||Glu
Given ||a ||serine ||and ||two ||ammonia ||molecules, ||hydrogen ||bonding ||exists ||between ||which ||
pairs ||of ||atoms? ||- ||CORRECT ||ANSWER✔✔-the ||H ||on ||the ||OH ||group ||on ||serine ||and ||the ||N: ||
on ||one ||ammonia ||molecule
Given ||the ||molecular ||masses ||of ||the ||following ||elements: ||H: ||1 ||Da, ||C: ||12 ||Da, ||N: ||14 ||Da, ||O:
||16 ||Da, ||which ||element ||is ||the ||most ||abundant ||in ||Aspartate ||at ||pH ||7.0 ||by ||weight?
a. ||oxygen
b. ||hydrogen
c. ||carbon
d. ||nitrogen ||- ||CORRECT ||ANSWER✔✔-a. ||oxygen
Which ||of ||the ||following ||is ||TRUE ||about ||mass ||spectrometry?
1) ||Velocity ||is ||inversely ||proportional ||to ||the ||mass
2) ||The ||detector ||can ||yield ||mass ||measurement ||up ||to ||3 ||significant ||figures
3)It ||can ||be ||used ||to ||quantify ||proteins
4)Samples ||need ||to ||be ||neutral ||- ||CORRECT ||ANSWER✔✔-1) ||Velocity ||is ||inversely ||
proportional ||to ||the ||mass
, How ||can ||you ||determine ||how ||many ||peptide ||bonds ||are ||present ||in ||the ||peptide ||shown ||
below? ||- ||CORRECT ||ANSWER✔✔-count ||the ||number ||of ||amide ||bonds: ||between ||double ||
bonded ||O ||and ||NH ||group
An ||atom ||with ||a ||lone ||pair ||of ||electrons ||can ||use ||it ||in ||different ||ways. ||When ||the ||lone ||pair
||of ||electrons ||is ||shared ||with ||another ||electron ||deficient ||atom ||to ||make ||a ||new ||bond, ||it ||is ||
acting ||as ||a ||______________. ||- ||CORRECT ||ANSWER✔✔-nucleophile
Give ||an ||example ||of ||an ||amino ||acid ||that ||contains ||a ||sulfur ||atom. ||Use ||the ||three-letter-
codes. ||- ||CORRECT ||ANSWER✔✔-Cys ||(or ||Met)
At ||pH ||1, ||which ||of ||the ||following ||is ||the ||predominant ||form ||of ||Lys? ||- ||CORRECT ||
ANSWER✔✔-should ||have ||NH3+ ||on ||the ||left
COOH ||on ||the ||right
CH2 ||(x4)
NH3+ ||on ||the ||bottom
Partition ||or ||thin ||layer ||chromatography ||separates ||proteins ||on ||the ||basis ||of ||polarity ||with ||
polar ||amino ||acids ||being ||retained ||on ||the ||stationary ||phase. ||Rank ||the ||following ||in ||order, ||
with ||least ||mobile ||amino ||acid ||first. ||- ||CORRECT ||ANSWER✔✔-1. ||His
2. ||Asn
3. ||Cys
4. ||Ile
A ||mixture ||of ||proteins ||contains ||Trypsinogen ||(24 ||kDa), ||fumerase ||(49 ||kDa), ||Transferrin ||(80 ||
kDa) ||and ||Aprotinin ||(6.5 ||kDa). ||Rank ||these ||proteins ||based ||on ||the ||order ||of ||their ||elution ||
from ||a ||gel ||filtration ||column. ||(1 ||being ||the ||first ||one ||to ||elute ||and ||4 ||as ||the ||last ||one) ||- ||
CORRECT ||ANSWER✔✔-1. ||transferrin
2. ||fumerase
3. ||trypsinogen
