BIOC 2580 EXAM QUESTIONS WITH CORRECT ANSWERS

BIOC 2580 EXAM QUESTIONS WITH
CORRECT ANSWERS
Lower ||pH ||means ||a ||________ ||proton ||concentration ||- ||CORRECT ||ANSWER✔✔-higher



If ||pH ||is ||one ||unit ||or ||more ||higher ||than ||pKa, ||the ||group ||is ||fully ||____________ ||- ||CORRECT
||ANSWER✔✔-deprotonated




If ||pH ||is ||equal ||to ||pKa, ||the ||group ||is ||__% ||deprotonated ||and ||__%

protonated ||- ||CORRECT ||ANSWER✔✔-50%, ||50%



If ||pH ||is ||one ||unit ||or ||more ||below ||the ||pK ||a ||, ||the ||group ||is ||fully ||_________ ||- ||CORRECT ||
ANSWER✔✔-protonated



The ||relationship ||of ||pH ||and ||pKa ||tells ||you ||whether ||a ||group ||is ||__________ ||or ||
____________ ||NOT ||whether ||it ||is ||positive ||or ||negative ||- ||CORRECT ||ANSWER✔✔-protonated
||or ||deprotonated




Amino ||acid ||analysis ||helps ||to ||determine ||_______ ||_________ ||- ||CORRECT ||ANSWER✔✔-
protein ||structure



What ||two ||processes ||are ||involved ||in ||amino ||acid ||analysis? ||- ||CORRECT ||ANSWER✔✔-(1) ||
Separation ||of ||a ||mixture ||into ||components

(2) ||Detection ||of ||the ||components ||of ||interest



The ||highest ||point ||reached ||by ||the ||solvent ||in ||thin ||layer ||chromatography ||is ||called ||the ||
_______ ||_____ ||- ||CORRECT ||ANSWER✔✔-solvent ||front

, In ||thin ||layer ||chromatography, ||very ||polar ||amino ||acids ||have ||___ ||Rf ||and ||non ||polar ||amino ||
acids ||have ||____ ||Rf ||- ||CORRECT ||ANSWER✔✔-low, ||high



Each ||amino ||acid ||can ||be ||identified ||by ||its ||characteristic ||________ ||________ ||in ||thin ||layer ||
chromatography ||- ||CORRECT ||ANSWER✔✔-relative ||mobility



The ||compound ||lowest ||on ||the ||plastic ||sheet ||in ||thin ||layer ||chromatography ||is ||the ||most ||
_____ ||- ||CORRECT ||ANSWER✔✔-polar



What ||two ||phases ||are ||involved ||in ||partition ||chromatography? ||- ||CORRECT ||ANSWER✔✔-
Stationary ||and ||mobile ||phase



Non ||polar ||compounds ||have ||a ||lower ||_________ ||volume ||in ||column ||chromatography ||- ||
CORRECT ||ANSWER✔✔-ellution



Ion ||exchange ||chromatography ||separates ||on ||the ||basis ||of ||______ ||- ||CORRECT ||ANSWER✔✔-
charge



Cation ||exchanger ||resins ||contain ||________ ||groups, ||which ||binds ||________ ||molecules ||
(cation ||exchange ||chromatography) ||- ||CORRECT ||ANSWER✔✔-negative, ||positive



Anion ||exchanger ||resins ||contain ||________ ||groups, ||which ||binds ||________ ||molecules ||
(anion ||exchange ||chromatography) ||- ||CORRECT ||ANSWER✔✔-positive, ||negative



Which ||two ||processes ||allows ||for ||the ||separation ||of ||proteins ||on ||the ||basis ||of ||size? ||- ||
CORRECT ||ANSWER✔✔-Gel ||filtration ||and ||molecular ||exclusion ||chromatography



Explain ||column ||chromatography ||using ||gel ||filtration ||- ||CORRECT ||ANSWER✔✔--Small ||
molecules ||penetrate ||the ||pores/beads ||and ||move ||slowly ||through ||the ||column