A phlebotomist should
remove the tourniquet :
A. Immediately after
needle insertion
B. As soon as blood
begins
C. After two tubes are
filled
D. After all tubes are
collected
B. As soon as blood flow begins
what does the term
"postprandial" mean?
A. After meals
B. Before meals
C. Before bedtime
D. After exercise
A. after meals
A new phlebotomist has
attempted venipuncture
twice on a patient but was
in successful both times .
What could she have
done differently to
improve her chances of a
successful draw?
A. Immediately try a third
stick in the same location
B. Ask another
phlebotomist for
assistance or consider
alternative sites
C. Reapply the tourniquet
more tightly to force the
vein rise
D. Ask the patient to drink
water immediately before
another attempt
B. Ask another phlebotomist for assistance or consider
alternative sites
Which PPE is always
required when performing
venipuncture with HIV?
A. Gown
B. Gloves and gown
C. Gloves only
D. Face shield
C. Gloves only
Which specimen should
be kept on ice during
transport?
A. Bilirubin
B. Ammonia
C. Potassium
D. Hemoglobin A1C
B. Ammonia
Which test requires
protection from light?
A. CBC
B. Glucose
C. PT/IRN
D. Bilirubin
D. Bilirubin
What is the first thing as
phlebotomist should do
when approaching a
patient?
A. Put on gloved
B. Wash hands
C. Identify the patient
D. Assemble supplies
C. Identify the patient
What is the best angle for
needle insertion during
venipuncture?
A. 15°- 30°
B. 5°- 10°
C. 45° - 60°
D. 60° - 90°
A. 15° - 30°
Why are two sets of blood
cultures collected from
two different sites?
A. To reduce the cost of
testing
B. To make the patient
more comfortable
C. To ensure faster lab
processing
D. To confirm infection
and rule out
contamination
D. to confirm infection and rule out contamination
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
1/18
When collecting a
capillary sample, what is
the preferred site for an
adult?
A. Earlobe
B. Thumb
C Index finger
D. Middle of ring finger
D. Middle or ring finger
What is the most common
site for venipuncture ?
A. radial artery
B. Brachial vein
C. median cubital vein
D. Femoral vein
C. median cubital vein
Which additive is found in
a lavender top tube?
A. Sodium citrate
B. EDTA
C. Heparin
D. Fluoride
B. EDTA
Which is the correct order
of draw for vacutainer
tubes ?
A. Red, light blue, green,
lavender, gray
B. Light blue, red, green,
lavender, gray
C. Light blue, red,
lavender, green, gray
D. Red, green, light blue,
gray, lavender
B. Light blue, red, green, lavender, gray
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
2/18
Which of the following is
used to clean a site before
drawing blood cultures?
A. Isopropyl alcohol
B. Hydrogen peroxide
C. Chlorhexidine
D. Soa and water
C. Chlorhexidine
Why is it important to
follow the correct order
of draw during
venipuncture?
A. To speed up the
process of collection
B. To ensure patient
comfort
C. To use fewer supplies
D. To avoid cross-
contamination between
different tube additives
D. To avoid cross-contamination between different
additives
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
3/18
A patient has an IV
Heparin in the right arm.
The patient has an AV
Fistula in the left arm -
which means it cannot be
used for lab draws. You
are asked to draw a PT
and PTT. What is the best
course of action?
A. Draw the sample above
the heparin IV line
B. Draw the sample from
the same arm but far from
the IV site
C. Draw the sample from
the left arm
D. Consult the nurse of
provider before drawing
blood from the arm with
the IV Heparin
D. Consult the nurse or provider before drawing
blood from the arm with the IV Heparin
When performing a heel
stick on an infant, the site
should be:
A. The center of the heel
B. The arch of the foot
C. The lateral or medial
heel
D. The big toes
C. the lateral or medial heel
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
4/18
The purpose of inversion
after collecting blood in
tubes with additives is to:
A. Increase oxygen
B. Prevent clotting or mix
properly
C. reduce hemolysis
D. Decrease glucose
levels
B. Prevent clotting or mix properly
which tube should be
drawn first when using
butterfly and collecting a
light blue tube?
A. Discard tube
B. Red
C. Lavender
D. Green
A. Discard tube
Which condition requires
the use of contact
precautions?
A. Tuberculosis
B. C. difficile
C. Influenza
D. Chickenpox
B. C. difficile
What is the ideal time
frame between collecting
the first and second set of
blood cultures?
A. Immediately one after
the other
B. At least 24 hours apart
C. 10-15 minutes apart or
based on provider order
D. One in the morning,
one in the evening
C. 10-15 mins apart or based on provider order
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
5/18
What does the term
"anemia" mean?
A. Excess white blood
cells
B. Low hemoglobin
C. Low platelets.
D. High cholesterol
B. Low hemoglobin
What is the main purpose
of using a centrifuge in the
laboratory?
A. To sterilize blood
samples
B. To measure blood
pressure
C. To warm blood samples
D. To separate
components of blood
D. To separate components of blood
A short draw in a light
blue tube can result in:
A. Hemolysis
B. Elevated glucose
C. Clotted Sample
D. Incorrect coagulation
results
D. Incorrect coagulation result
Which blood component
is responsible for clotting?
A. Plasma
B. Platelets
C. Red blood cells
D. White blood cells
B.
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
6/18
After completing a
venipuncture, what should
the phlebotomist do with
the used needle?
A. Recap the needle and
place it in a sharps
container
B. Place the needle
directly in the sharps
container without
recapping
C. leave the needle in the
tray for biohazard pickup
D. Dispose of it in a
regular trash if no blood is
visible
B. Place the needle directly in the sharps without
recapping
7/23/25, 1:58 AM Phlebotomy Certification Practice Test
7/18
If a hematoma begins to
form, you should:
A. Continue drawing
B. Apply a band aid
C. Stop the draw and
apply pressure
D. Ignore it
C. Stop the draw and apply pressure
which instruction should
be given to a patient
preparing for a fasting
blood glucose test?
A. Do not eat or drink
anything except water for
8-12 hours
B. Eat normally before the
test
C. Avoid water and food
for 24 hours
D. Drink orange juice an
hour before the test
A. Do not eat or drink anything except water for 8-12
hours
What is the function of the
pulmonary vein?
A. Carry oxygenated
blood from lungs to heart
B. Carry deoxygenated
blood from blood body to
lungs
C. Carry blood to the liver
D. Carry blood to the
brain
A. Carry oxygenated blood from the lungs to the
heart
Content preview
Phlebotomy Certification Practice Test bank
exam 2025-2026 \complete questions and
accurate detailed answers \latest update
A phlebotomist should B. As soon as blood flow begins
remove the tourniquet :
A. Immediately after
needle insertion
B. As soon as blood
begins
C. After two tubes are
filled
D. After all tubes are
collected
what does the term A. after meals
"postprandial" mean?
A. After meals
B. Before meals
C. Before bedtime
D. After exercise
,A new phlebotomist has B. Ask another phlebotomist for assistance or consider
attempted venipuncture alternative sites
twice on a patient but was
in successful both times .
What could she have
done differently to
improve her chances of a
successful draw?
A. Immediately try a third
stick in the same location
B. Ask another
phlebotomist for
assistance or consider
alternative sites
C. Reapply the tourniquet
more tightly to force the
vein rise
D. Ask the patient to drink
water immediately before
another attempt
Which PPE is always C. Gloves only
required when performing
venipuncture with HIV?
A. Gown
B. Gloves and gown
C. Gloves only
D. Face shield
Which specimen should B. Ammonia
be kept on ice during
transport?
A. Bilirubin
B. Ammonia
C. Potassium
D. Hemoglobin A1C
, Which test requires D. Bilirubin
protection from light?
A. CBC
B. Glucose
C. PT/IRN
D. Bilirubin
What is the first thing as C. Identify the patient
phlebotomist should do
when approaching a
patient?
A. Put on gloved
B. Wash hands
C. Identify the patient
D. Assemble supplies
What is the best angle for A. 15° - 30°
needle insertion during
venipuncture?
A. 15°- 30°
B. 5°- 10°
C. 45° - 60°
D. 60° - 90°
Why are two sets of blood D. to confirm infection and rule out contamination
cultures collected from
two different sites?
A. To reduce the cost of
testing
B. To make the patient
more comfortable
C. To ensure faster lab
processing
D. To confirm infection
and rule out
contamination