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C785 Biochemistry preassessment Questions
and Answers (Expert Solutions)
Q: Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles?
ANS 🗹🗹: 16
Q: What is the function of DNA polymerase in the process of PCR?
ANS 🗹🗹: It recognizes the primers and uses the available dNTPs to replicate the
template DNA sequence.
Q: What are possible blood types of the parents of a patient with blood type AB?
ANS 🗹🗹: A and B
Q: 115 116 117 118 − Amino Acid Number
Tyr Gly Thr Pro − Amino Acid Sequence
TAC GGG ACA CCA − DNA Sequence
What is the amino acid in position 118 that results from a single nucleotide insertion
immediately following the three guanines that encode for glycine 116?
ANS 🗹🗹: Thr
Q: In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to
valine (Val).
What change in codons corresponds to this mutation?
ANS 🗹🗹: GAA to GTA
Q: Which type of mammalian DNA damage repair requires the presence of the
chromosome that is homologous to the damaged chromosome?
ANS 🗹🗹: Recombination
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Q: Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or
more of the genes that perform nucleotide excision repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the general
population?
ANS 🗹🗹: The mutation rate of all other genes is higher due to failure to repair.
Q: Which mRNA sequence results in a polypeptide that contains three amino acids?
ANS 🗹🗹: 5' - CUU GAU ACU UAG - 3'
Q: Which template strand DNA sequence would result in the amino acid (Ser)?
ANS 🗹🗹: 5' - CGA - 3'
Q: What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
ANS 🗹🗹: 5' - ATT CGA GAT GGC - 3'
Q: Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is
associated with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to
cocaine increases their expression?
ANS 🗹🗹: The nucleosomes become more widely spaced.
Q: What occurs during the process of alternative splicing of mRNA?
ANS 🗹🗹: Alternate combinations of exons within the same gene are linked
together.
Q: Which force is most influential in determining the tertiary structure of a protein?
ANS 🗹🗹: Hydrophobic effect
Q: Given the following amino acid structure, what is the strongest intermolecular force
it would participate in to stabilize a protein structure?
C785 Biochemistry preassessment Questions
and Answers (Expert Solutions)
Q: Assuming 100% reaction efficiency, how many DNA copies are created after the
completion of four complete PCR cycles?
ANS 🗹🗹: 16
Q: What is the function of DNA polymerase in the process of PCR?
ANS 🗹🗹: It recognizes the primers and uses the available dNTPs to replicate the
template DNA sequence.
Q: What are possible blood types of the parents of a patient with blood type AB?
ANS 🗹🗹: A and B
Q: 115 116 117 118 − Amino Acid Number
Tyr Gly Thr Pro − Amino Acid Sequence
TAC GGG ACA CCA − DNA Sequence
What is the amino acid in position 118 that results from a single nucleotide insertion
immediately following the three guanines that encode for glycine 116?
ANS 🗹🗹: Thr
Q: In sickle-cell disease, the glutamic acid (Glu) at position number seven is mutated to
valine (Val).
What change in codons corresponds to this mutation?
ANS 🗹🗹: GAA to GTA
Q: Which type of mammalian DNA damage repair requires the presence of the
chromosome that is homologous to the damaged chromosome?
ANS 🗹🗹: Recombination
, Page | 2
Q: Xeroderma pigmentosum (XP) is a recessive genetic disease that occurs when one or
more of the genes that perform nucleotide excision repair are nonfunctional.
Why do XP patients have a much higher incidence of skin cancer than the general
population?
ANS 🗹🗹: The mutation rate of all other genes is higher due to failure to repair.
Q: Which mRNA sequence results in a polypeptide that contains three amino acids?
ANS 🗹🗹: 5' - CUU GAU ACU UAG - 3'
Q: Which template strand DNA sequence would result in the amino acid (Ser)?
ANS 🗹🗹: 5' - CGA - 3'
Q: What is the complementary sequence for the following DNA sequence?
5' - GCC ATC TCG AAT - 3'
ANS 🗹🗹: 5' - ATT CGA GAT GGC - 3'
Q: Studies have shown that increased expression of two genes called SIRT1 and SIRT2 is
associated with chronic exposure to cocaine and contributes to drug addiction.
What difference in the regions of the SIRT1 and SIRT2 genes in people addicted to
cocaine increases their expression?
ANS 🗹🗹: The nucleosomes become more widely spaced.
Q: What occurs during the process of alternative splicing of mRNA?
ANS 🗹🗹: Alternate combinations of exons within the same gene are linked
together.
Q: Which force is most influential in determining the tertiary structure of a protein?
ANS 🗹🗹: Hydrophobic effect
Q: Given the following amino acid structure, what is the strongest intermolecular force
it would participate in to stabilize a protein structure?
