NURS 6521 Case Study - Oil Spill Bioremediation Laboratory

Oil Spill Bioremediation Laboratory Introduction Background Species, including plants and fish, maybe harmed by oil spills, both in the ocean and on land. Dolphins, whales, seals, and sea otters have succumbed to hypothermia after exposure to oil spills (Talvenmäki, 2021). Oil spills may contaminate an animal's food source. Oil spills in the water are one of the most common tragedies across the planet. According to recent research, the oil spill's consequences still affect deep marine creatures in the region where the tragedy occurred (Nandy, 2021). Objectives Oil degradation is one of the numerous subjects being researched at this facility. Research in this lab aims to figure out how oxygen interacts with microbial solutions in the wild. This laboratory investigates the viscosity of water to see whether the oil would degrade or become apparent after a few days. Hypotheses Tube 1 There should be no cloudiness in the liquid in tube 1. Since the solution was clear, I expected the liquid to be transparent. By the end of the fourth day, I believe there will be no oil recovered. Because I think the solution will be free of oil, I don't expect to detect any. None of this oil is expected to show up until the fourth day. 3 Tube 2 Assuming that the vial contains an object with a bit of quantity of color, I'm assuming that the solution will take on the object's hue. As predicted, the oil will begin to deteriorate after three days. I saw tiny bubbles in the oil after adding all the ingredients and shaking the container with my index finger. Over the following three days, I expect the oil to degrade. Tube 3 Tube 3 should stay clean, at least according to my hypothesis. All of my components are transparent, and I am sure that the vial liquid will remain transparent. I'm sure that there will be no indication of oil after four days. I'm sure that there will be no oil in the vial because of the large amount of clear solution that was put into it. Tube 4 My hopes for the fourth tube are that this sample has a hint of fog in it. I'm anticipating a very thin mist to form since one of the solutions in the vial has a slight tint to it. A gradual decomposition of the oil will occur, but ultimately it will happen. I hypothesize that the old will begin to crumble gradually since so few oil bubbles were at the top. Tube 5 This is the most complex sample to evaluate, in my view. This sample is the least obvious due to the tetrazolium and microbiological solution inclusions. My conclusion is that oil will degrade more quickly than the other samples. This tube seemed to have a more considerable oil buildup than the others. Therefore, I expect the oil to break down the quickest in this one. 4 Tube 6 My best guess is that Tube 6 will be the second to worst for fog. It felt like this one would be a bit lighter than tube five, but not as hazy as tube five, from the way the color developed. This oil degradation is expected to speed up significantly during several days. Because of how obvious and bubbly it was when it landed, I fully anticipated it to break down over the following days. Because of this, I believe it will. Materials and Methods Six tubes and two 100 ml plastic cups were used in this experiment that included tetrazolium and rid-x powder and oil and tap water for the investigation. A cheesecloth split in half was used as a filter for the rid-x power in the funnel. Next, I took a plastic cup and began filling it with water. A funnel was used to collect the rid-x powder. I used tap water to fill the 100 ml cylinder and pulled out 140 ml to get an accurate result. After that, I drank a 100-ml cylinder of water. After metering out the water with the funnel, I added the rid-x powder to the water in my cup. After putting the water in, I let it sit in the cup with the funnel for about 15 minutes. I used deionized water in the brown container since I had it on hand and adequately mixed it. After that, I numbered each tube from 1 to 6. The next step was to add 2 ml of distilled water to tubes 1, 2, 3, and 4. Instead of using the old one, I put 10 drops of oil into each tube 1, 2, 3, 4, 5, and 6 before throwing it away. I disposed of the pipette after that. A second pipet was used to draw 2ml of water into tube 1. After sucking up some water, put 3 ml of water in tube 3. After that, I disposed of the pipette. My pipette had 2ml of the bacteria solution, so I added tubes 2, 4, 5, and 6 using a syringe. 5 I disposed of the pipette after that. A fresh pipet was used to transfer the tetrazolium solution to tubes 1, 2, 5, and 6. After completing the tubes' filling, I used six caps to cover each one before closing it. After closing each tube, I shook it with my finger in a circular motion. As an experiment to test how fast or slowly the oil degraded, I left the tubes exposed to light and removed the caps one by one. I re-opened the tubes, checked the samples, and re-tightened the caps the next day. Results Data Table 1. Tubes 1 and 2 Observations Day Tube 1 Tube 2 0 (Initial setup) The liquid was clear and the oil was settled at the top. The color of the liquid was a clear foggy color. 1 The liquid was still clear and the oil was still settled at the top. The foggy color of the liquid had gotten a bit stronger. The oil was still settled at the top. 2 no change Still foggy, oil is starting to break down. 3 there is no signs of oil and solution is still clear Still very foggy, oil is almost brokendown fully