ASCP CG Exam (Classic Cytogenetics) with correct anwers 2025
What are the basics of in situ hybridization? - correct answerPretreatment of slides with acid,
extraction of DNA with barium hydroxide, and incubation in 2xSSC to promote denaturation.
The following are indicators for amniocentesis except: - correct answerA family history of an
autosomal recessive disorder.
When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample should: -
correct answerBe put in media and kept at room temp.
A PB sample is clotted. What do you do? - correct answerBreak up the clot.
All of the following information is required during the specimen set up procedure except: -
correct answerInfectious agent.
When should samples be logged in? - correct answerAs soon as they arrive in the laboratory.
How long do you wait before requesting an amniotic fluid if no cell attachment is observed? -
correct answer10 days.
All of the following will result in culture failure except: - correct answerTransport at room temp.
Which cells would you want to use for long term serial cytogenetic studies? - correct
answerFibroblasts.
The following enzymes can be used to dissociate chorionic villus samples except for: - correct
answerRNAse.
,Which of the following is not a necessary ingredient which is added to modified medium? -
correct answerAntibiotics.
All of the following have buffering capability except: - correct answerL-Glutamine.
What type of media is necessary for the culture of Fragile X cases? - correct answerFolic acid
deficient media.
The most important reason why Biological Safety Caninets are used instead of Horizontal
Laminar Flow Hoods in the handling of human tissues is: - correct answerTo protect the
technologist who is processing the specimen.
In which of the following situations would one suspect bacterial contamination? - correct
answerThe culture media is turbid in appearance.
Which of the following can be used for serial culture from amniotic fluid? - correct
answerFibroblasts.
Which of the following chemicals is used for cryopreservation? - correct answerDimethyl
sulfoxide (DMSO).
Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to occur
except: - correct answerCell swelling.
During slidemaking all of the following factors are important except for: - correct answerSlides
are 1st being dipped in acetic acid.
A good banding pattern will be expected when we observe chromosomes before banding under
the phase scope with the following appearance: - correct answerDark.
Which of the following factors are not important for chromosome spreading? - correct
answerMitotic index.
,The best method to process slides for G-banding on the same day that they are dropped is: -
correct answerIncubate the slide in 90°C for 1-3 hrs before banding.
You are analyzing a clinical case and discover a small extra marker with satellites. What staining
technique would be most useful in determining the chromosomal origin of this marker? -
correct answerDAPI/DA.
When using a fluorescein labeled probe during the FISH procedure, the appropriate counter
stain used should be: - correct answerPropidium iodide.
When doing sequential staining which of the following is used to remove oil from slides? -
correct answerXylene.
What is the best banding technique to distinguish between 47,XY,+18 and 47,XYY? - correct
answerQFQ.
When the identification of paired chromosomes is the primary goal, which if the following
banding techniques should be used? - correct answerG-banding.
When the addition of non homologous material to the q-arm of chromosome 16 is suspected
after G-banding, which if the following banding techniques should be used? - correct answerC-
banding.
Which chromosome is most different between C- and G-banding? - correct answer9.
What is not a criteria for G-band identification? - correct answerLocation of the telomere.
What do quinacrine and acridine orange dyes stain? - correct answerAT rich DNA.
, What type of container should be used for collecting peripheral blood and bone marrow
specimens? - correct answerGreen top tube coated with sodium heparin.
What type of container should be used for collecting solid tissue samples? - correct answerA
sterile container containing media, supplemented with antibiotics.
How do you avoid microbial contamination? - correct answerBy using aseptic technique, sterile
containers and antibiotics/fungicides.
How do you avoid cellular contamination of prenatal specimens? - correct answerDiscard the
1st 1-2 cc of amniotic fluid drawn.
How do you avoid cellular contamination of solid tumor specimens? - correct answerDissect
away normal parenchyma and fatty tissue.
How much specimen is required for the successful analysis of a peripheral blood specimen? -
correct answer5-10 ml for adults and 3-5 ml for children.
How much specimen is required for the successful analysis of a bone marrow specimen? -
correct answer1-3 ml.
How much specimen is required for the successful analysis of a prenatal specimen? - correct
answer15-30 ml of amniotic fluid collected in two separate containers.
How much specimen is required for the successful analysis of solid tissues? - correct answer5-25
mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid tumor.
What should the label of each collection container always have? - correct answerPatient name
and identification number.
What does penicillin/streptomycin treat? - correct answerBacterial contamination.
What are the basics of in situ hybridization? - correct answerPretreatment of slides with acid,
extraction of DNA with barium hydroxide, and incubation in 2xSSC to promote denaturation.
The following are indicators for amniocentesis except: - correct answerA family history of an
autosomal recessive disorder.
When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample should: -
correct answerBe put in media and kept at room temp.
A PB sample is clotted. What do you do? - correct answerBreak up the clot.
All of the following information is required during the specimen set up procedure except: -
correct answerInfectious agent.
When should samples be logged in? - correct answerAs soon as they arrive in the laboratory.
How long do you wait before requesting an amniotic fluid if no cell attachment is observed? -
correct answer10 days.
All of the following will result in culture failure except: - correct answerTransport at room temp.
Which cells would you want to use for long term serial cytogenetic studies? - correct
answerFibroblasts.
The following enzymes can be used to dissociate chorionic villus samples except for: - correct
answerRNAse.
,Which of the following is not a necessary ingredient which is added to modified medium? -
correct answerAntibiotics.
All of the following have buffering capability except: - correct answerL-Glutamine.
What type of media is necessary for the culture of Fragile X cases? - correct answerFolic acid
deficient media.
The most important reason why Biological Safety Caninets are used instead of Horizontal
Laminar Flow Hoods in the handling of human tissues is: - correct answerTo protect the
technologist who is processing the specimen.
In which of the following situations would one suspect bacterial contamination? - correct
answerThe culture media is turbid in appearance.
Which of the following can be used for serial culture from amniotic fluid? - correct
answerFibroblasts.
Which of the following chemicals is used for cryopreservation? - correct answerDimethyl
sulfoxide (DMSO).
Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to occur
except: - correct answerCell swelling.
During slidemaking all of the following factors are important except for: - correct answerSlides
are 1st being dipped in acetic acid.
A good banding pattern will be expected when we observe chromosomes before banding under
the phase scope with the following appearance: - correct answerDark.
Which of the following factors are not important for chromosome spreading? - correct
answerMitotic index.
,The best method to process slides for G-banding on the same day that they are dropped is: -
correct answerIncubate the slide in 90°C for 1-3 hrs before banding.
You are analyzing a clinical case and discover a small extra marker with satellites. What staining
technique would be most useful in determining the chromosomal origin of this marker? -
correct answerDAPI/DA.
When using a fluorescein labeled probe during the FISH procedure, the appropriate counter
stain used should be: - correct answerPropidium iodide.
When doing sequential staining which of the following is used to remove oil from slides? -
correct answerXylene.
What is the best banding technique to distinguish between 47,XY,+18 and 47,XYY? - correct
answerQFQ.
When the identification of paired chromosomes is the primary goal, which if the following
banding techniques should be used? - correct answerG-banding.
When the addition of non homologous material to the q-arm of chromosome 16 is suspected
after G-banding, which if the following banding techniques should be used? - correct answerC-
banding.
Which chromosome is most different between C- and G-banding? - correct answer9.
What is not a criteria for G-band identification? - correct answerLocation of the telomere.
What do quinacrine and acridine orange dyes stain? - correct answerAT rich DNA.
, What type of container should be used for collecting peripheral blood and bone marrow
specimens? - correct answerGreen top tube coated with sodium heparin.
What type of container should be used for collecting solid tissue samples? - correct answerA
sterile container containing media, supplemented with antibiotics.
How do you avoid microbial contamination? - correct answerBy using aseptic technique, sterile
containers and antibiotics/fungicides.
How do you avoid cellular contamination of prenatal specimens? - correct answerDiscard the
1st 1-2 cc of amniotic fluid drawn.
How do you avoid cellular contamination of solid tumor specimens? - correct answerDissect
away normal parenchyma and fatty tissue.
How much specimen is required for the successful analysis of a peripheral blood specimen? -
correct answer5-10 ml for adults and 3-5 ml for children.
How much specimen is required for the successful analysis of a bone marrow specimen? -
correct answer1-3 ml.
How much specimen is required for the successful analysis of a prenatal specimen? - correct
answer15-30 ml of amniotic fluid collected in two separate containers.
How much specimen is required for the successful analysis of solid tissues? - correct answer5-25
mg of CVS, 1 mm^3 of skin biopsy and 1cm^3 of solid tumor.
What should the label of each collection container always have? - correct answerPatient name
and identification number.
What does penicillin/streptomycin treat? - correct answerBacterial contamination.
