Micro Lab midterm Question and answers rated A+ 2025
1. Gram- cells stain purple whereas 4. What is hea
gram- cells stain pink or red when fixation?
using the Gram stain technique. A) Passing
A) positive; negative the slide
B) negative; positive through
the flame
2. The Gram stain is an example of a of a burner
several
staining procedure, which takes advantage times after
of the fact that cells or parts of cells react the smear
differently and can be distinguished by has
the use of two different dyes. completely
A) differential dried.
B) simple B) The
C)special heating
D) basic of the
inoculati
3. Select the statements that represent goals in n loop
after
correct smear preparation.
removal
1. The cells must be heat fixed to the slide to
prevent removal when stain is washed off
the slide.
2. It is important to minimize any artifacts
or distor- tion of the cells for accurate
viewing after staining.
3. Smears must be thick in order to easily
take up stains.
4. Organisms should be killed on the
inoculating loop before application to the
slide.
5. Smears must be thin to allow for
observation of single cells.
,Micro Lab midterm Question and answers rated A+ 2025
A) positive; negative
A) ditterential
1, 2, 5
A) Passing the slide through the flame of a burner several times after
,Micro Lab midterm Question and answers rated A+ 2025
of the organisms to be applied to the slide. the smear has completely
C)The heating of the inoculation loop before removal dried.
of the organisms to be applied to the slide.
D)Heating the slide until it is red-hot to kill
the organ- isms before staining.
5. How does smear preparation of cells from a A) Water is applied to
liquid medium differ from preparation of the slide before
cells from a solid medium? emulsify- ing cells from
A) Water is applied to the slide before a solid medi- um
emulsifying cells from a solid medium.
B) Stain is applied to the cells in the culture
tube and then, they are applied to the slide.
C)Heat fixation is used to evaporate the liquid
medi- um.
D) A needle is used instead of an inoculating
loop.
6. Order the reagents used in the acid-fast staining pro- C-1
cedure.
-Carbolfuchsin 3
-acid-alcohol Methylene
blue
7. What makes Mycobacterium particularly A) The presence of
resistant to staining? mycolic acid in their
A) The presence of mycolic acid in their cell cell walls
walls
B) The presence of endospores
C)Their lack of a cell wall to trap the dye
molecules
D) Their susceptibility to lysis when heat is
applied
,Micro Lab midterm Question and answers rated A+ 2025
8. The primary mode of action of non ionizing radiation A) make
pyrimidine dimers
is to...
A) make pyrimidine dimers
1. Gram- cells stain purple whereas 4. What is hea
gram- cells stain pink or red when fixation?
using the Gram stain technique. A) Passing
A) positive; negative the slide
B) negative; positive through
the flame
2. The Gram stain is an example of a of a burner
several
staining procedure, which takes advantage times after
of the fact that cells or parts of cells react the smear
differently and can be distinguished by has
the use of two different dyes. completely
A) differential dried.
B) simple B) The
C)special heating
D) basic of the
inoculati
3. Select the statements that represent goals in n loop
after
correct smear preparation.
removal
1. The cells must be heat fixed to the slide to
prevent removal when stain is washed off
the slide.
2. It is important to minimize any artifacts
or distor- tion of the cells for accurate
viewing after staining.
3. Smears must be thick in order to easily
take up stains.
4. Organisms should be killed on the
inoculating loop before application to the
slide.
5. Smears must be thin to allow for
observation of single cells.
,Micro Lab midterm Question and answers rated A+ 2025
A) positive; negative
A) ditterential
1, 2, 5
A) Passing the slide through the flame of a burner several times after
,Micro Lab midterm Question and answers rated A+ 2025
of the organisms to be applied to the slide. the smear has completely
C)The heating of the inoculation loop before removal dried.
of the organisms to be applied to the slide.
D)Heating the slide until it is red-hot to kill
the organ- isms before staining.
5. How does smear preparation of cells from a A) Water is applied to
liquid medium differ from preparation of the slide before
cells from a solid medium? emulsify- ing cells from
A) Water is applied to the slide before a solid medi- um
emulsifying cells from a solid medium.
B) Stain is applied to the cells in the culture
tube and then, they are applied to the slide.
C)Heat fixation is used to evaporate the liquid
medi- um.
D) A needle is used instead of an inoculating
loop.
6. Order the reagents used in the acid-fast staining pro- C-1
cedure.
-Carbolfuchsin 3
-acid-alcohol Methylene
blue
7. What makes Mycobacterium particularly A) The presence of
resistant to staining? mycolic acid in their
A) The presence of mycolic acid in their cell cell walls
walls
B) The presence of endospores
C)Their lack of a cell wall to trap the dye
molecules
D) Their susceptibility to lysis when heat is
applied
,Micro Lab midterm Question and answers rated A+ 2025
8. The primary mode of action of non ionizing radiation A) make
pyrimidine dimers
is to...
A) make pyrimidine dimers
