BCH403 FINAL TEST QUESTIONS
WITH CORRECT ANSWERS
E1: Which of the following statements is true regarding the hydrophobic effect? -
Answer- Non - Polar molecules are driven together in order to maximize the entropy of
water.
E1: The peptide in question 17 is dissolved into a solution that has a pH of 6.5. Under
these conditions the net charge of the peptide is - Answer- +1
E1: A glycine residue is disruptive to the stability of an a-helix or a b-strand because: -
Answer- It's R-group is too small to participate in stabilizing interactions with
neighboring R - groups
E1: With a pKa of 6.37 the carbonic acid/bicarbonate system serves to buffer changes
to the pH of blood. The acid dissociates as follows:
H2CO3 + H20 <-> HCO3- + H30+
If a solution of 0.5M carbonic acid is adjusted to a pH of 2.5, the primary form of the
buffer will be: - Answer- H2CO3
E1: Most nonpolar residues are located on the interior of a protein fold, but are
occasionally found on surfaces where they can mediate protein interactions. The
molecular basis of a hypothetical infectious disease is that a bacterial protein interacts
with a human protein by sharing a nonpolar surface. Your goal as a researcher is to find
a way to disrupt this interaction. Which of the following surface mutations are most likely
to disrupt the protein interactions? - Answer- Leucine --> Glutamine
E1: You've discovered a new species of rodent living at a very high altitude, where the
partial pressure of oxygen is low. What would you predict regarding the oxygen binding
capacity of this new species? - Answer- B and D ( it's hemoglobin will have a very low
P50 value & the primary amino acid sequence of its hemoglobin will not be identical to
that of a species living in an oxygen rich atmosphere)
E3: Which one of the following molecules can control glycogen synthesis and glycogen
breakdown on a per cell basis? - Answer- Glucose-6-phosphate
E3: Human pyruvate dehydrogenase complex is regulated by all the following molecules
except ___________? - Answer- Glucagon
, E3: How many electrons does each cytochrome C protein donates complex IV? -
Answer- 1
E3: Which component of the F0 subunit rotates? - Answer- c subunit
E3: Anaerobic catabolism of glucose results in the formation of: - Answer- 2 ATP
E3: How many molecules of O2 can be completely reduced to H2O using electrons
donated from NADH generated by glycolysis (assume 1 molecule of glucose)? -
Answer- 1
E4: Two different lipases are required to cleave all three fatty acid side chains from
triacylglycerol stored in adipose tissue. - Answer- FALSE
E4: Transport of fatty acids into the mitochondria is the main regulatory step of beta-
Oxidation. - Answer- TRUE
E4: Vitamin D3 can be synthesized from cholesterol. - Answer- FALSE
E4: Because we are able to store nitrogen as urea, we do not need to constantly
replenish our amino acids from our diet. - Answer- FALSE
E4: Biochemical starvation caused by the absence of pancreatic beta-cells is classified
as Type I Diabetes. - Answer- TRUE
E4: Chylomicron is synthesized in mucosal cells of the small intestine. - Answer- TRUE
E4: The liver is the only tissue in the body that can use ketone bodies for fuel. - Answer-
FALSE
E4: Glycerol-3-phosphate acyltransferase is the regulatory step of triacylglycerol
biosynthesis. - Answer- TRUE
E4: Cardiac muscles prefer fatty acids as their primary fuel source. - Answer- TRUE
E4: Acetyl-CoA can be used to synthesize glucose during gluconeogenesis. - Answer-
FALSE
E4: Which one of the following enzymes is capable of cleaving all three fatty acid side
chains off of a glycerol backbone? - Answer- Lipoprotein lipase
E4: Non-palmitate fatty acid biosynthesis occurs in which organelle of the cell? -
Answer- Endoplasmic reticulum
WITH CORRECT ANSWERS
E1: Which of the following statements is true regarding the hydrophobic effect? -
Answer- Non - Polar molecules are driven together in order to maximize the entropy of
water.
E1: The peptide in question 17 is dissolved into a solution that has a pH of 6.5. Under
these conditions the net charge of the peptide is - Answer- +1
E1: A glycine residue is disruptive to the stability of an a-helix or a b-strand because: -
Answer- It's R-group is too small to participate in stabilizing interactions with
neighboring R - groups
E1: With a pKa of 6.37 the carbonic acid/bicarbonate system serves to buffer changes
to the pH of blood. The acid dissociates as follows:
H2CO3 + H20 <-> HCO3- + H30+
If a solution of 0.5M carbonic acid is adjusted to a pH of 2.5, the primary form of the
buffer will be: - Answer- H2CO3
E1: Most nonpolar residues are located on the interior of a protein fold, but are
occasionally found on surfaces where they can mediate protein interactions. The
molecular basis of a hypothetical infectious disease is that a bacterial protein interacts
with a human protein by sharing a nonpolar surface. Your goal as a researcher is to find
a way to disrupt this interaction. Which of the following surface mutations are most likely
to disrupt the protein interactions? - Answer- Leucine --> Glutamine
E1: You've discovered a new species of rodent living at a very high altitude, where the
partial pressure of oxygen is low. What would you predict regarding the oxygen binding
capacity of this new species? - Answer- B and D ( it's hemoglobin will have a very low
P50 value & the primary amino acid sequence of its hemoglobin will not be identical to
that of a species living in an oxygen rich atmosphere)
E3: Which one of the following molecules can control glycogen synthesis and glycogen
breakdown on a per cell basis? - Answer- Glucose-6-phosphate
E3: Human pyruvate dehydrogenase complex is regulated by all the following molecules
except ___________? - Answer- Glucagon
, E3: How many electrons does each cytochrome C protein donates complex IV? -
Answer- 1
E3: Which component of the F0 subunit rotates? - Answer- c subunit
E3: Anaerobic catabolism of glucose results in the formation of: - Answer- 2 ATP
E3: How many molecules of O2 can be completely reduced to H2O using electrons
donated from NADH generated by glycolysis (assume 1 molecule of glucose)? -
Answer- 1
E4: Two different lipases are required to cleave all three fatty acid side chains from
triacylglycerol stored in adipose tissue. - Answer- FALSE
E4: Transport of fatty acids into the mitochondria is the main regulatory step of beta-
Oxidation. - Answer- TRUE
E4: Vitamin D3 can be synthesized from cholesterol. - Answer- FALSE
E4: Because we are able to store nitrogen as urea, we do not need to constantly
replenish our amino acids from our diet. - Answer- FALSE
E4: Biochemical starvation caused by the absence of pancreatic beta-cells is classified
as Type I Diabetes. - Answer- TRUE
E4: Chylomicron is synthesized in mucosal cells of the small intestine. - Answer- TRUE
E4: The liver is the only tissue in the body that can use ketone bodies for fuel. - Answer-
FALSE
E4: Glycerol-3-phosphate acyltransferase is the regulatory step of triacylglycerol
biosynthesis. - Answer- TRUE
E4: Cardiac muscles prefer fatty acids as their primary fuel source. - Answer- TRUE
E4: Acetyl-CoA can be used to synthesize glucose during gluconeogenesis. - Answer-
FALSE
E4: Which one of the following enzymes is capable of cleaving all three fatty acid side
chains off of a glycerol backbone? - Answer- Lipoprotein lipase
E4: Non-palmitate fatty acid biosynthesis occurs in which organelle of the cell? -
Answer- Endoplasmic reticulum
