Transcription
1) Below is a
double stranded
DNA molecule
that codes for
two genes. The
template
sequence of gene 1 is read from left to right. The template sequence for gene 2 is read
from right to left. Which strand of DNA (top or bottom) serves as the template for each
gene? Explain.
a) The bottom strand is the template for gene 1 and the top strand is the template
for gene 2 because DNA is read 3’ to 5’ to allow for transcription to occur 5’ to 3’.
This leads to RNA polymerase moving left to right for gene 1, and right to left for
gene 2.
2) The sequences that code for the 5’ UTR in a gene are located in a(n)
a. promoter
b. exon
c. intron
3) Any given DNA protein coding sequence has 3 possible reading frames and the correct
one is set by an initiator codon
1. What will be the translation of mRNA GCAGCGAGA?
a. Ala-Ala-Arg
2. The DNA template strand of a gene contains the sequence
DNA 5’-TTCAGTCGT-3’. Draw mRNA sequence. Then
translate it.
a. mRNA 3’- AAGUCAGCA-5’
b. RNA 5’-ACGACUGAA-3’ (have to flip to translate it)
i. Thr Thr Glu
1) Below is a
double stranded
DNA molecule
that codes for
two genes. The
template
sequence of gene 1 is read from left to right. The template sequence for gene 2 is read
from right to left. Which strand of DNA (top or bottom) serves as the template for each
gene? Explain.
a) The bottom strand is the template for gene 1 and the top strand is the template
for gene 2 because DNA is read 3’ to 5’ to allow for transcription to occur 5’ to 3’.
This leads to RNA polymerase moving left to right for gene 1, and right to left for
gene 2.
2) The sequences that code for the 5’ UTR in a gene are located in a(n)
a. promoter
b. exon
c. intron
3) Any given DNA protein coding sequence has 3 possible reading frames and the correct
one is set by an initiator codon
1. What will be the translation of mRNA GCAGCGAGA?
a. Ala-Ala-Arg
2. The DNA template strand of a gene contains the sequence
DNA 5’-TTCAGTCGT-3’. Draw mRNA sequence. Then
translate it.
a. mRNA 3’- AAGUCAGCA-5’
b. RNA 5’-ACGACUGAA-3’ (have to flip to translate it)
i. Thr Thr Glu
