Bio 111 lab Tamu Exam 1 UPDATED
ACTUAL QUESTIONS AND CORRECT
ANSWERS
Accuracy - CORRECT ANSWER✅✅a measure of how close a result matches a
correct or standard value
Precision - CORRECT ANSWER✅✅a measure of variability. For example, three
darts placed close together on a dartboard indicate high precision, but if this cluster
of darts is far from the bullseye it would also indicate low accuracy
Aliquot - CORRECT ANSWER✅✅a portion of something (noun); or to divide or
transfer a portion of something (verb). For this lab, aliquot refers to an amount of
liquid
Micropipette - CORRECT ANSWER✅✅an instrument used to measure/deliver
amounts of liquid with high accuracy and precision. Micropipettes are expensive so
treat these with respect
Spectrophotometer - CORRECT ANSWER✅✅an instrument for measuring the
intensity of light that is transmitted through a sample (transmittance) or absorbed by
a sample (absorbance). In this lab, you will measure light absorbance at a defined
wavelength
Molar weight - CORRECT ANSWER✅✅a measure of mass. By definition, a mole
is 6x1023 objects
Standard curve - CORRECT ANSWER✅✅a graph in which properties of known
samples are measured and plotted. The direct proportionality of these properties can
then be used to determine the same information from unknown samples. In this lab,
a standard curve of Absorbance versus protein concentration will be used to
determine the concentration of protein in an unknown sample.
Concentration - CORRECT ANSWER✅✅C1V1=C2V2
Enzyme - CORRECT ANSWER✅✅molecules which catalyze biochemical reaction
and bind specific reactants, referred to as substrates.
Enzyme activity - CORRECT ANSWER✅✅is affected by the concentration of both
enzyme and substrates, temp, and pH.
β-Galactosidase (β-Gal) - CORRECT ANSWER✅✅an enzyme produced by
Escherichia coli (E. coli) that catalyzes the hydrolysis (breakdown of a molecule by
using up a water molecule) of the disaccharide lactose to the monosaccharides
galactose and glucose
ACTUAL QUESTIONS AND CORRECT
ANSWERS
Accuracy - CORRECT ANSWER✅✅a measure of how close a result matches a
correct or standard value
Precision - CORRECT ANSWER✅✅a measure of variability. For example, three
darts placed close together on a dartboard indicate high precision, but if this cluster
of darts is far from the bullseye it would also indicate low accuracy
Aliquot - CORRECT ANSWER✅✅a portion of something (noun); or to divide or
transfer a portion of something (verb). For this lab, aliquot refers to an amount of
liquid
Micropipette - CORRECT ANSWER✅✅an instrument used to measure/deliver
amounts of liquid with high accuracy and precision. Micropipettes are expensive so
treat these with respect
Spectrophotometer - CORRECT ANSWER✅✅an instrument for measuring the
intensity of light that is transmitted through a sample (transmittance) or absorbed by
a sample (absorbance). In this lab, you will measure light absorbance at a defined
wavelength
Molar weight - CORRECT ANSWER✅✅a measure of mass. By definition, a mole
is 6x1023 objects
Standard curve - CORRECT ANSWER✅✅a graph in which properties of known
samples are measured and plotted. The direct proportionality of these properties can
then be used to determine the same information from unknown samples. In this lab,
a standard curve of Absorbance versus protein concentration will be used to
determine the concentration of protein in an unknown sample.
Concentration - CORRECT ANSWER✅✅C1V1=C2V2
Enzyme - CORRECT ANSWER✅✅molecules which catalyze biochemical reaction
and bind specific reactants, referred to as substrates.
Enzyme activity - CORRECT ANSWER✅✅is affected by the concentration of both
enzyme and substrates, temp, and pH.
β-Galactosidase (β-Gal) - CORRECT ANSWER✅✅an enzyme produced by
Escherichia coli (E. coli) that catalyzes the hydrolysis (breakdown of a molecule by
using up a water molecule) of the disaccharide lactose to the monosaccharides
galactose and glucose
