Histology Ascp Exam Actual Exam Questions With Complete Solution Guide) (Multiple Choices) (A+ Graded 100% Verified) Latest Version 2025

Histology ASCP Exam ACTUAL EXAM QUESTIONS
WITH COMPLETE SOLUTION GUIDE) (MULTIPLE
CHOICES) (A+ GRADED 100% VERIFIED) LATEST
VERSION 2025
An example of an additive is one that contains:

A. Picric acid

B. Acetic acid

C. Ethyl alcohol

D. Acetone - ...(ANSWERS)....Picric Acid



When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will
show:

A. Better ultrastructural preservation

B. Decreased immunoreactivity

C. Increased enzyme activity

D. Superior nuclear detail - ...(ANSWERS)....Superior nuclear detail



Microscopic examination of an H&E stained section fixed in formalin shows
marked nuclear bubbling. One most often sees this artifact if the specimen is
processed following:

A. Incomplete fixation

B. Prolonged fixation

C. Microwave fixation

,D. Frozen sectioning - ...(ANSWERS)....Incomplete fixation



Microscopic evaluation of H&E stained sections from a surgically removed small
bowel specimen shows an absence of much of the epithelium in otherwise normal
tissue. This most likely resulted from:

A. Mechanical trauma

B. Delayed fixation

C. Ulceration

D. Poor choice of fixative - ...(ANSWERS)....Delayed fixation



A specimen of kidney must be shipped to another city for immunofluorescence
studies. The specimen should be placed in:

A. Saline

B. Michel solution

C. Buffered formalin

D. Orth solution - ...(ANSWERS)....Michel solution



A certain project requires a fixative that contains acetic acid yet stabilizes
erythrocyte membranes. One fixative that could be used is:

A. Zenker solution

B. Bouin solution

C. Gendre solution

D. Hollande solution - ...(ANSWERS)....Hollande solution

,When the microwave oven is used for fixation, the most critical factor is the:

A. Preparation of the formalin solution

B. Use of glass containers

C. Control of the temperature

D. Osmolality of the fixative solution - ...(ANSWERS)....Control of the temperature



To adequately remove the calcium in a specimen containing areas of
microcalcification, the tissue could be fixed in:

A. Hollande solution

B. NBF

C. B-5 solution

D. Zamboni solution - ...(ANSWERS)....Hollande solution



What fixative contains copper acetate?

A. Hollande

B. Bouin

C. Gendre

D. Zamboni - ...(ANSWERS)....Hollande



A specimen is submitted with the statement that it was fixed in formalin.
Microscopic sections show marked lysis of erythrocytes. This indicates that the
fixative most likely was:

, A. Prepared with too much formalin

B. Buffered above neutrality

C. Acidified with acetic acid

D. Not formalin - ...(ANSWERS)....Acidified with acetic acid



Fixatives are classified as additive because of the:

A. Addition of several chemicals to the solution

B. Addition, or binding of the fixative to tissue proteins

C. Additional reactions occurring with longer fixation

D. Additional reactive tissue sites available for dye binding -
...(ANSWERS)....Addition, or binding of the fixative to tissue proteins



Kidney biopsy tissue has been fixed in phosphate-buffered glutaraldehyde for 2
hours and then placed in phosphate buffer solution. If a portion of this tissue is
processed for light microscopy, sections would most likely show:

A. Very poor glomerular preservation

B. Decreased uptake of hematoxylin

C. Lysis of cytoplasmic elements

D. Nonspecific PAS staining - ...(ANSWERS)....Nonspecific PAS staining



Uric acid crystals are preserved ONLY when tissue is fixed in:

A. Absolute alcohol

B. NBF