MLT 106 UNIT 4 EXAM 2025 QUESTIONS
AND ANSWERS
What is the technique in which one excludes antibodies that could not be responsible for
reactivity seen? What is advised? - ANS rule out technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What phases are tested? -
ANS O cells in 3 phases of testing (IS, 37, AHG)
What is RESt? - ANS rabbit erythrocyte stroma; removes insignificant antibodies
If phenotyping is difficult, what may be performed? - ANS homologous adsorption
What is AHG? What is the difference between mono- and polyspecific? - ANS antihuman
globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - ANS hybridoma - mouse spleen
lymphocyte with myeloma
What produces polyclonal antibodies? - ANS rabbits; inject animals and harvest antibodies
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, What is the difference between IAT and DAT? - ANS in vitro - in a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - ANS transfusion within 2 weeks
history of antibodies
pregnancy
Do you use a DAT or IAT for cord blood studies in mother and neonate compatibility? -
ANS DAT
For what patients would you run an antibody evaluation? - ANS patients with a history of
clinically significant antibodies
How do you confirm validity of antibody identification? - ANS Phenotype
Which method checks to see if the pattern of antigen-positive cells matches the pattern of
reactivity? - ANS Inclusion
Which method is based on negative results to rule out antibodies? - ANS Exclusion
Does adsorption, elution, or neutralization use the individual's own cells due to presence of an
interfering antibody when trying to find unexpected antibodies? - ANS Adsorption
If a person is anemic and their own cells cannot be used, what do you do? - ANS homologous
adsorption
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AND ANSWERS
What is the technique in which one excludes antibodies that could not be responsible for
reactivity seen? What is advised? - ANS rule out technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What phases are tested? -
ANS O cells in 3 phases of testing (IS, 37, AHG)
What is RESt? - ANS rabbit erythrocyte stroma; removes insignificant antibodies
If phenotyping is difficult, what may be performed? - ANS homologous adsorption
What is AHG? What is the difference between mono- and polyspecific? - ANS antihuman
globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - ANS hybridoma - mouse spleen
lymphocyte with myeloma
What produces polyclonal antibodies? - ANS rabbits; inject animals and harvest antibodies
COPYRIGHT © 2025 THESTAR ALL RIGHTS RESERVED 1
, What is the difference between IAT and DAT? - ANS in vitro - in a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - ANS transfusion within 2 weeks
history of antibodies
pregnancy
Do you use a DAT or IAT for cord blood studies in mother and neonate compatibility? -
ANS DAT
For what patients would you run an antibody evaluation? - ANS patients with a history of
clinically significant antibodies
How do you confirm validity of antibody identification? - ANS Phenotype
Which method checks to see if the pattern of antigen-positive cells matches the pattern of
reactivity? - ANS Inclusion
Which method is based on negative results to rule out antibodies? - ANS Exclusion
Does adsorption, elution, or neutralization use the individual's own cells due to presence of an
interfering antibody when trying to find unexpected antibodies? - ANS Adsorption
If a person is anemic and their own cells cannot be used, what do you do? - ANS homologous
adsorption
COPYRIGHT © 2025 THESTAR ALL RIGHTS RESERVED 2
