ASCP CG Certification Practice \|\| \|\| \|\| \|\|
QUESTIONS WITH ANSWERS \|\| \|\|
What is the best agent to use against mycoplasma? -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔BM cyclin - a combination of two
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
antibiotics (tiamulin and minocycline) that effecticely targets
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
mycoplasma growth \|\|
For successful harvest, each new batch of hypotonic solution
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
should be evaluated by: - CORRECT ANSWERS ✔✔comparing
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the new lot to a lot already in use
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
Colagenase, pronase, and trypsin are: - CORRECT ANSWERS \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔Proteolytic enzymes - break down tissues into single-cell \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
suspensions
When harvesting peripheral blood cultures, asceptic technique
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
no longer matters following incubation with: - CORRECT
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
ANSWERS ✔✔Colcemid - mitotic spindle poison that halts cells
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
at metaphase
\|\|
Samples that arrive at the cytogenetics lab should first be: -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔Logged in to the system \|\| \|\| \|\| \|\| \|\| \|\|
Phytohemagglutinin (PHA) stimulates which type of cells into \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
division? - CORRECT ANSWERS ✔✔T-cell lymphocytes
\|\| \|\| \|\| \|\| \|\|
,To avoid culture failure, monitoring reagents is crucial. When
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
logging a culture failure, what is the MOST important to
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
document? - CORRECT ANSWERS ✔✔Expiration dates of \|\| \|\| \|\| \|\| \|\| \|\| \|\|
reagents used \|\|
Which type of microscope is used when cleaning a CVS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(chorionic villus) sample? - CORRECT ANSWERS ✔✔Dissection
\|\| \|\| \|\| \|\| \|\| \|\|
Which cell type is most problematic if found in a CVS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(chorionic villus) sample? - CORRECT ANSWERS ✔✔Decidua
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
(maternal tissue) \|\|
When looking at a slide under a phase contrast scope and a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
lot of cytoplasm is still seen around the mets, what is the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
most likely cause? - CORRECT ANSWERS ✔✔innappropriate
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
slide drying time
\|\| \|\|
What will help prevent microbial contamination and cross
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
contamination of cultures? - CORRECT ANSWERS ✔✔Asceptic \|\| \|\| \|\| \|\| \|\| \|\| \|\|
technique
When there are not enough mets to complete the study of a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
patient/case, what needs to be done next? - CORRECT \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
ANSWERS ✔✔prepare additional slides from any remaining
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
sample
When looking at a dropped slide under a phase scope and
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
mets are very spread out and sparse, what could help fix the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
,issue? - CORRECT ANSWERS ✔✔Increading the drying time by
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
using warm slides\|\| \|\|
What is true of an inverted microscope? - CORRECT ANSWERS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔the objectives are beneath the stage
\|\| \|\| \|\| \|\| \|\|
High-resolution banding is indicated for a possible diagnosis \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
of: - CORRECT ANSWERS ✔✔DiGenorge syndrome --
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
microdeletion of 22q11, also called velocardiofacial syndrome \|\| \|\| \|\| \|\| \|\| \|\| \|\|
"velo" - soft palette, \|\| \|\| \|\| \|\|
"cardio" - congenital heart defects, \|\| \|\| \|\| \|\| \|\|
"facial" - unique abnormal facial features, short philtrum
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(upper lip indent), tubular nose, wide spread eyes, etc
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
When verifying if a specimen is appropriate for cytogenetic
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
analysis, what is the MOST important data to accompany the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
specimen? - CORRECT ANSWERS ✔✔Reason for referral \|\| \|\| \|\| \|\| \|\| \|\|
Which type of specimen should be requested for a cytogenetic
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
analysis for turner syndrome? - CORRECT ANSWERS
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔Heparinized peripheral blood stored at room temperature \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(NOT FROZEN) \|\|
, A blood sample can be used to study the cytogenetics of a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
malignancy only if: - CORRECT ANSWERS ✔✔Dividing leukemic \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
cells are present in circulation
\|\| \|\| \|\| \|\|
All of the cultures in a CO2 incubator failed, what aspects of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the incubator are important to monitor to assess the source of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the problem? - CORRECT ANSWERS ✔✔Temperature,
\|\| \|\| \|\| \|\| \|\| \|\|
CO2 level, and \|\| \|\|
Humidity level \|\|
The most important factor affecting the quality of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
chromosomes viewed under a phase contrast scope is: - \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔Slide drying after the cells have been \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
dropped
What is the modal number (default number of chromosomes
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
in a cell) for a Down syndrome translocation carrier? -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔45 \|\| \|\|
What is the best tissue to use for both a good success rate
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
and abnormality detection in spontaneous abortion
\|\| \|\| \|\| \|\| \|\| \|\|
miscarriages? - CORRECT ANSWERS ✔✔Chorionic villi \|\| \|\| \|\| \|\| \|\|
What is a possible cause for a drying oven to not maintain a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
proper temperature? - CORRECT ANSWERS ✔✔The door does
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
not seal properly
\|\| \|\|
QUESTIONS WITH ANSWERS \|\| \|\|
What is the best agent to use against mycoplasma? -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔BM cyclin - a combination of two
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
antibiotics (tiamulin and minocycline) that effecticely targets
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
mycoplasma growth \|\|
For successful harvest, each new batch of hypotonic solution
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
should be evaluated by: - CORRECT ANSWERS ✔✔comparing
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the new lot to a lot already in use
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
Colagenase, pronase, and trypsin are: - CORRECT ANSWERS \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔Proteolytic enzymes - break down tissues into single-cell \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
suspensions
When harvesting peripheral blood cultures, asceptic technique
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
no longer matters following incubation with: - CORRECT
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
ANSWERS ✔✔Colcemid - mitotic spindle poison that halts cells
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
at metaphase
\|\|
Samples that arrive at the cytogenetics lab should first be: -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔Logged in to the system \|\| \|\| \|\| \|\| \|\| \|\|
Phytohemagglutinin (PHA) stimulates which type of cells into \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
division? - CORRECT ANSWERS ✔✔T-cell lymphocytes
\|\| \|\| \|\| \|\| \|\|
,To avoid culture failure, monitoring reagents is crucial. When
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
logging a culture failure, what is the MOST important to
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
document? - CORRECT ANSWERS ✔✔Expiration dates of \|\| \|\| \|\| \|\| \|\| \|\| \|\|
reagents used \|\|
Which type of microscope is used when cleaning a CVS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(chorionic villus) sample? - CORRECT ANSWERS ✔✔Dissection
\|\| \|\| \|\| \|\| \|\| \|\|
Which cell type is most problematic if found in a CVS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(chorionic villus) sample? - CORRECT ANSWERS ✔✔Decidua
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
(maternal tissue) \|\|
When looking at a slide under a phase contrast scope and a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
lot of cytoplasm is still seen around the mets, what is the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
most likely cause? - CORRECT ANSWERS ✔✔innappropriate
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
slide drying time
\|\| \|\|
What will help prevent microbial contamination and cross
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
contamination of cultures? - CORRECT ANSWERS ✔✔Asceptic \|\| \|\| \|\| \|\| \|\| \|\| \|\|
technique
When there are not enough mets to complete the study of a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
patient/case, what needs to be done next? - CORRECT \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
ANSWERS ✔✔prepare additional slides from any remaining
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
sample
When looking at a dropped slide under a phase scope and
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
mets are very spread out and sparse, what could help fix the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
,issue? - CORRECT ANSWERS ✔✔Increading the drying time by
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
using warm slides\|\| \|\|
What is true of an inverted microscope? - CORRECT ANSWERS
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔the objectives are beneath the stage
\|\| \|\| \|\| \|\| \|\|
High-resolution banding is indicated for a possible diagnosis \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
of: - CORRECT ANSWERS ✔✔DiGenorge syndrome --
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
microdeletion of 22q11, also called velocardiofacial syndrome \|\| \|\| \|\| \|\| \|\| \|\| \|\|
"velo" - soft palette, \|\| \|\| \|\| \|\|
"cardio" - congenital heart defects, \|\| \|\| \|\| \|\| \|\|
"facial" - unique abnormal facial features, short philtrum
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(upper lip indent), tubular nose, wide spread eyes, etc
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
When verifying if a specimen is appropriate for cytogenetic
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
analysis, what is the MOST important data to accompany the
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
specimen? - CORRECT ANSWERS ✔✔Reason for referral \|\| \|\| \|\| \|\| \|\| \|\|
Which type of specimen should be requested for a cytogenetic
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
analysis for turner syndrome? - CORRECT ANSWERS
\|\| \|\| \|\| \|\| \|\| \|\| \|\|
✔✔Heparinized peripheral blood stored at room temperature \|\| \|\| \|\| \|\| \|\| \|\| \|\|
(NOT FROZEN) \|\|
, A blood sample can be used to study the cytogenetics of a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
malignancy only if: - CORRECT ANSWERS ✔✔Dividing leukemic \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
cells are present in circulation
\|\| \|\| \|\| \|\|
All of the cultures in a CO2 incubator failed, what aspects of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the incubator are important to monitor to assess the source of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
the problem? - CORRECT ANSWERS ✔✔Temperature,
\|\| \|\| \|\| \|\| \|\| \|\|
CO2 level, and \|\| \|\|
Humidity level \|\|
The most important factor affecting the quality of
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
chromosomes viewed under a phase contrast scope is: - \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔Slide drying after the cells have been \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
dropped
What is the modal number (default number of chromosomes
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
in a cell) for a Down syndrome translocation carrier? -
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
CORRECT ANSWERS ✔✔45 \|\| \|\|
What is the best tissue to use for both a good success rate
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
and abnormality detection in spontaneous abortion
\|\| \|\| \|\| \|\| \|\| \|\|
miscarriages? - CORRECT ANSWERS ✔✔Chorionic villi \|\| \|\| \|\| \|\| \|\|
What is a possible cause for a drying oven to not maintain a
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
proper temperature? - CORRECT ANSWERS ✔✔The door does
\|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
not seal properly
\|\| \|\|
