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CPT Exam Study Guide – Certified Phlebotomy Technician 2025/2026 Full Q&A and Procedural Review

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This comprehensive CPT (Certified Phlebotomy Technician) study guide for the 2025/2026 exam includes detailed questions and answers covering all core topics. Content spans infection control (PPE, isolation precautions), venipuncture procedures, tube color and additive identification, blood draw order, patient safety, sample handling, legal responsibilities, anatomy, emergency protocols, and lab values. Designed for students preparing for national certification exams, it ensures readiness through clinical definitions, protocols, and testing criteria. Keywords: CPT exam 2025 phlebotomy study guide venipuncture order tube color additives anticoagulants PPE protocols N95 mask isolation precautions quality assurance blood culture GTT ABG transport hematoma prevention pediatric heel stick capillary draw sequence STAT blood draw phlebotomy emergencies

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CPT - Certified Phlebotomy
Technician exam 2025/2026



What mask needs to be worn in a room with airborne isolation precautions - Answer--N-95 Mask (plus
gloves & a gown)



What does PPE stand for - Answer--Personal Protective Equipment



What PPE needs to be worn in a room with droplet isolation precautions - Answer--Gloves, mask, eye
protection & gown



What PPE needs to be worn in a room with contact precautions - Answer--Gloves & gown



What gauge needle will cause hemolysis - Answer--25 gauge (and above) (to small & it breaks the blood
up; to large and it slams into the back of the tube)



What is the range of needle gauges for blood donation - Answer--16-18 gauge



Needles for phlebotomy should be pitched how many degrees - Answer--15o-30 degree angle

, Needles should be inserted how far - Answer--¼"- ½" or until you reach a change of resistance



What are the three vessels of the anti cubital fossa - Answer--Median Cubital Vein, Cephalic Vein,
Brachial/basilic Vein



Which vessel is the first choice for a blood draw - Answer--Median Cubital Vein



Which vessel is the second choice for a blood draw - Answer--Cephalic Vein



Which vessel is the third choice for a blood draw - Answer--Brachial/basilic Vein

What is an example of invasion of privacy - Answer--Looking at medical records without permission,
talking about a patient



What are three examples when chain-of-custody is paramount - Answer--Blood alcohol level/drug tests,
rape tests/sexual assault, paternity



What is the term for the standards that would best be described as quality control, instrument
maintenance, and procedure manuals - Answer--Quality Assurance (QA)



A phlebotomist's job includes greeting and instructing patients. True or False - Answer--True



A phlebotomist's job includes performing QA on laboratory equipment. True or False - Answer--True



It is sufficient to explain a procedure to a minor, and not their parent. True or False - Answer--False

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