MLT 106 Unit 4 2025/2026 Exam
Questions and Corresponding Answers
with Surety of 100% Pass Mark
What is the technique in which one excludes antibodies that could not be
responsible for reactivity seen? What is advised? - 🧠ANSWER ✔✔rule out
technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What
phases are tested? - 🧠ANSWER ✔✔O cells in 3 phases of testing (IS, 37,
AHG)
What is RESt? - 🧠ANSWER ✔✔rabbit erythrocyte stroma; removes
insignificant antibodies
If phenotyping is difficult, what may be performed? - 🧠ANSWER
✔✔homologous adsorption
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
1
,What is AHG? What is the difference between mono- and polyspecific? -
🧠ANSWER ✔✔antihuman globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - 🧠ANSWER
✔✔hybridoma - mouse spleen lymphocyte with myeloma
What produces polyclonal antibodies? - 🧠ANSWER ✔✔rabbits; inject
animals and harvest antibodies
What is the difference between IAT and DAT? - 🧠ANSWER ✔✔in vitro - in
a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - 🧠ANSWER ✔✔transfusion within 2 weeks
history of antibodies
pregnancy
Do you use a DAT or IAT for cord blood studies in mother and neonate
compatibility? - 🧠ANSWER ✔✔DAT
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
2
, For what patients would you run an antibody evaluation? - 🧠ANSWER
✔✔patients with a history of clinically significant antibodies
How do you confirm validity of antibody identification? - 🧠ANSWER
✔✔Phenotype
Which method checks to see if the pattern of antigen-positive cells matches
the pattern of reactivity? - 🧠ANSWER ✔✔Inclusion
Which method is based on negative results to rule out antibodies? -
🧠ANSWER ✔✔Exclusion
Does adsorption, elution, or neutralization use the individual's own cells
due to presence of an interfering antibody when trying to find unexpected
antibodies? - 🧠ANSWER ✔✔Adsorption
If a person is anemic and their own cells cannot be used, what do you do? -
🧠ANSWER ✔✔homologous adsorption
If you cannot use their own cells because they had a recent transfusion and
have a positive DAT, what do you do? - 🧠ANSWER ✔✔differential
adsorption
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
3
Questions and Corresponding Answers
with Surety of 100% Pass Mark
What is the technique in which one excludes antibodies that could not be
responsible for reactivity seen? What is advised? - 🧠ANSWER ✔✔rule out
technique
only for homozygous expression
What type of manufactured cells are used in panel evaluation? What
phases are tested? - 🧠ANSWER ✔✔O cells in 3 phases of testing (IS, 37,
AHG)
What is RESt? - 🧠ANSWER ✔✔rabbit erythrocyte stroma; removes
insignificant antibodies
If phenotyping is difficult, what may be performed? - 🧠ANSWER
✔✔homologous adsorption
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
1
,What is AHG? What is the difference between mono- and polyspecific? -
🧠ANSWER ✔✔antihuman globulin; green bottle reagent
mono has specific complement or anti-IgG
poly contains several
What technology produces monoclonal antibodies? - 🧠ANSWER
✔✔hybridoma - mouse spleen lymphocyte with myeloma
What produces polyclonal antibodies? - 🧠ANSWER ✔✔rabbits; inject
animals and harvest antibodies
What is the difference between IAT and DAT? - 🧠ANSWER ✔✔in vitro - in
a tube
in vivo - in the body (no incubation phase)
Why would you run a DAT? - 🧠ANSWER ✔✔transfusion within 2 weeks
history of antibodies
pregnancy
Do you use a DAT or IAT for cord blood studies in mother and neonate
compatibility? - 🧠ANSWER ✔✔DAT
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
2
, For what patients would you run an antibody evaluation? - 🧠ANSWER
✔✔patients with a history of clinically significant antibodies
How do you confirm validity of antibody identification? - 🧠ANSWER
✔✔Phenotype
Which method checks to see if the pattern of antigen-positive cells matches
the pattern of reactivity? - 🧠ANSWER ✔✔Inclusion
Which method is based on negative results to rule out antibodies? -
🧠ANSWER ✔✔Exclusion
Does adsorption, elution, or neutralization use the individual's own cells
due to presence of an interfering antibody when trying to find unexpected
antibodies? - 🧠ANSWER ✔✔Adsorption
If a person is anemic and their own cells cannot be used, what do you do? -
🧠ANSWER ✔✔homologous adsorption
If you cannot use their own cells because they had a recent transfusion and
have a positive DAT, what do you do? - 🧠ANSWER ✔✔differential
adsorption
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
3
