BICH 410 EXAM 2025 WITH ACCURATE SOLUTIONS
Due to 2, consecutive hydroxyls on the carbon backbone
(vicinal diol), ______ is vulnerable to hydrolysis,
Compared to it’s counterpart in DNA, ______, which has one
of the vicinal hydroxyls removed.
The synthetically-designed ______ has a both vicinal
hydroxyls removed, resulting in chain termination, during
DNA polymerization. – Answer ☑️ribose, deoxyribose,
dideoxyribose
Which of the following best describes an endergonic
reaction? – Answer ☑️It requires an input of energy to
proceed
All of the following are physical properties of water, EXCEPT:
High heat capacity
High boiling point
Cohesion and adhesion
Less dense as a liquid
,Excellent solvent properties – Answer ☑️Less dense as a
liquid
Which buffer would be most effective at maintaining a pH of
7.4 if you have access to acetic acid (pKa = 4.76) and
phosphoric acid (pKa = 7.2)? – Answer ☑️phosphoric acid
What causes the hydrophobic effect in aqueous solutions? –
Answer ☑️Water molecules forced to order in a structured
“cage” around nonpolar molecules
What is the ΔG (Gibbs free energy) for a reaction with the
following parameters?
ΔH = -40 kJ mol-1
ΔS = -0.1 kJ mol-1 K-1
T = 298 K – Answer ☑️ΔG = ΔH – TΔS = -40 – (298 × -0.1) = -
40 + 29.8 = -10.2 kJ/mol)
-10 kJ mol-1
,Which of the following makes “palindromic,” double-
stranded DNA? – Answer ☑️GATATC
Which of the following statements describes the structure of
DNA? – Answer ☑️A 2’ hydride and a methylated version of
adenine’s base-pair partner allow for greater stability of this
storage form of nucleic acids
Which of the following defines the polar functional groups
present in this amino-acid structure? – Answer ☑️Amino
group, carboxyl group, and hydroxyl group
Which of the following scientists below recognized the
helical structure of DNA based on X-ray diffraction images? –
Answer ☑️Francis Crick
What type of ends do some restriction enzymes generate
after cutting DNA? – Answer ☑️Sticky ends
Two reactions are coupled, both in the forward direction.
, One has ΔG°’ = -25 kJ mol-1 and the other has ΔG°’ = 15 kJ
mol-1.
What is the net ΔG°’ for the coupled process? – Answer ☑️-
10 kJ mol-1
Which of the following is a pyrimidine nucleotide component
of RNA? – Answer ☑️Uridine monophosphate
Using the convention for writing nucleic acid sequences,
what is the sequence of DNA complementary to the
sequence, below?
AGATCGGTATCAGGT – Answer ☑️ACCTGATACCGATCT
Which functional group serves as a hydrogen bond donor
from adenine, when it is part of a base pair? –
Answer ☑️Amino (-NH2)
Given the following parameters, what is the pH of the buffer
solution?
Due to 2, consecutive hydroxyls on the carbon backbone
(vicinal diol), ______ is vulnerable to hydrolysis,
Compared to it’s counterpart in DNA, ______, which has one
of the vicinal hydroxyls removed.
The synthetically-designed ______ has a both vicinal
hydroxyls removed, resulting in chain termination, during
DNA polymerization. – Answer ☑️ribose, deoxyribose,
dideoxyribose
Which of the following best describes an endergonic
reaction? – Answer ☑️It requires an input of energy to
proceed
All of the following are physical properties of water, EXCEPT:
High heat capacity
High boiling point
Cohesion and adhesion
Less dense as a liquid
,Excellent solvent properties – Answer ☑️Less dense as a
liquid
Which buffer would be most effective at maintaining a pH of
7.4 if you have access to acetic acid (pKa = 4.76) and
phosphoric acid (pKa = 7.2)? – Answer ☑️phosphoric acid
What causes the hydrophobic effect in aqueous solutions? –
Answer ☑️Water molecules forced to order in a structured
“cage” around nonpolar molecules
What is the ΔG (Gibbs free energy) for a reaction with the
following parameters?
ΔH = -40 kJ mol-1
ΔS = -0.1 kJ mol-1 K-1
T = 298 K – Answer ☑️ΔG = ΔH – TΔS = -40 – (298 × -0.1) = -
40 + 29.8 = -10.2 kJ/mol)
-10 kJ mol-1
,Which of the following makes “palindromic,” double-
stranded DNA? – Answer ☑️GATATC
Which of the following statements describes the structure of
DNA? – Answer ☑️A 2’ hydride and a methylated version of
adenine’s base-pair partner allow for greater stability of this
storage form of nucleic acids
Which of the following defines the polar functional groups
present in this amino-acid structure? – Answer ☑️Amino
group, carboxyl group, and hydroxyl group
Which of the following scientists below recognized the
helical structure of DNA based on X-ray diffraction images? –
Answer ☑️Francis Crick
What type of ends do some restriction enzymes generate
after cutting DNA? – Answer ☑️Sticky ends
Two reactions are coupled, both in the forward direction.
, One has ΔG°’ = -25 kJ mol-1 and the other has ΔG°’ = 15 kJ
mol-1.
What is the net ΔG°’ for the coupled process? – Answer ☑️-
10 kJ mol-1
Which of the following is a pyrimidine nucleotide component
of RNA? – Answer ☑️Uridine monophosphate
Using the convention for writing nucleic acid sequences,
what is the sequence of DNA complementary to the
sequence, below?
AGATCGGTATCAGGT – Answer ☑️ACCTGATACCGATCT
Which functional group serves as a hydrogen bond donor
from adenine, when it is part of a base pair? –
Answer ☑️Amino (-NH2)
Given the following parameters, what is the pH of the buffer
solution?
