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HESI A2 Biology Practice Questions – 80 Exam-Style Questions with Answers & Explanations

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Get exam-ready with this HESI A2 Biology Practice Question set, featuring 80 high-yield questions that mirror the real test. Topics include cell structure, DNA/RNA, genetics, metabolism, homeostasis, and human body systems. Each question includes a detailed answer and explanation to reinforce key biology concepts—ideal for nursing school applicants preparing for the HESI Admission Assessment Exam.

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HESI A2 Biology Practice Questions
80 Multiple-Choice Questions for Exam Preparation



1. Cell Structure and Function
1. What is the primary function of the mitochondria in a eukaryotic cell?
A. Protein synthesis
B. Energy production
C. DNA replication
D. Waste elimination
Correct Answer: B. Energy production
2. Which organelle is responsible for synthesizing proteins?
A. Golgi apparatus
B. Ribosome
C. Lysosome
D. Endoplasmic reticulum
Correct Answer: B. Ribosome
3. What structure regulates the movement of substances in and out of the cell?
A. Nucleus
B. Cell membrane
C. Cytoplasm
D. Vacuole
Correct Answer: B. Cell membrane
4. Which type of cell lacks a nucleus?
A. Eukaryotic
B. Prokaryotic
C. Animal
D. Plant
Correct Answer: B. Prokaryotic
5. What is the function of the Golgi apparatus?
A. Modifies, sorts, and packages proteins
B. Synthesizes carbohydrates
C. Breaks down cellular waste
D. Stores genetic information
Correct Answer: A. Modifies, sorts, and packages proteins
6. Which structure in plant cells provides structural support?
A. Chloroplast
B. Cell wall
C. Mitochondrion
D. Nucleus
Correct Answer: B. Cell wall


1

, 7. What is the primary role of lysosomes in a cell?
A. Energy production
B. Waste digestion
C. Protein transport
D. DNA storage
Correct Answer: B. Waste digestion
8. Which organelle contains chlorophyll in plant cells?
A. Mitochondrion
B. Chloroplast
C. Ribosome
D. Vacuole
Correct Answer: B. Chloroplast
9. What is the fluid component of the cytoplasm called?
A. Cytosol
B. Nucleoplasm
C. Matrix
D. Vesicle
Correct Answer: A. Cytosol
10. Which structure is the control center of a eukaryotic cell?
A. Endoplasmic reticulum
B. Nucleus
C. Mitochondrion
D. Centriole
Correct Answer: B. Nucleus


2. DNA and RNA
11. What is the primary role of DNA in a cell?
A. Protein synthesis
B. Energy storage
C. Genetic information storage
D. Cellular transport
Correct Answer: C. Genetic information storage
12. Which nitrogenous base is found in RNA but not in DNA?
A. Adenine
B. Thymine
C. Uracil
D. Guanine
Correct Answer: C. Uracil
13. What is the process of copying DNA into RNA called?
A. Translation
B. Transcription
C. Replication
D. Mutation
Correct Answer: B. Transcription

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