HESI A2 Biology Practice Exam 2025 - 80 Unique
Questions
Comprehensive Study Guide for Nursing School Applicants
Includes 80 Unique Practice Questions with Detailed Answer Key
Covers Cell Biology, Genetics, Evolution, Human Anatomy and Physiology,
Microbiology, and Ecology
Updated for the 2025 HESI A2 Exam
Contents
1 Instructions 2
2 Biology Practice Questions (80 Questions) 2
3 Answer Key with Explanations 12
1
, 1 Instructions
This practice exam prepares you for the Biology section of the HESI Admission Assess-
ment (A2) Exam 2025, a nursing school entrance requirement. It includes 80 multiple-
choice questions covering cell structure and function, genetics, evolution, human anatomy
and physiology, microbiology, and ecology, with a focus on nursing-relevant concepts. Se-
lect the best answer from the four options provided. An answer key with explanations is
included.
• Time Limit: Approximately 60–80 minutes, reflecting HESI A2 Biology section
pacing.
• Materials: No calculator required.
• Scoring: Each question is worth 1 point. No penalty for incorrect answers.
2 Biology Practice Questions (80 Questions)
1. What is the primary function of mitochondria in a cell?
a) Protein synthesis
b) Energy production
c) Cell division
d) Waste storage
Answer: b) Mitochondria produce ATP, the cells energy currency.
2. Which organ system regulates body temperature?
a) Nervous
b) Integumentary
c) Endocrine
d) Muscular
Answer: b) The integumentary system (skin) helps regulate temperature.
3. What is the role of DNA in a cell?
a) Energy storage
b) Genetic information storage
c) Cell membrane protection
d) Protein transport
Answer: b) DNA stores genetic instructions for cell functions.
4. Which blood vessel carries oxygenated blood from the lungs to the heart?
a) Pulmonary artery
b) Pulmonary vein
c) Aorta
d) Vena cava
Answer: b) The pulmonary vein carries oxygenated blood.
5. What is the basic unit of heredity?
a) Chromosome
b) Gene
2
Questions
Comprehensive Study Guide for Nursing School Applicants
Includes 80 Unique Practice Questions with Detailed Answer Key
Covers Cell Biology, Genetics, Evolution, Human Anatomy and Physiology,
Microbiology, and Ecology
Updated for the 2025 HESI A2 Exam
Contents
1 Instructions 2
2 Biology Practice Questions (80 Questions) 2
3 Answer Key with Explanations 12
1
, 1 Instructions
This practice exam prepares you for the Biology section of the HESI Admission Assess-
ment (A2) Exam 2025, a nursing school entrance requirement. It includes 80 multiple-
choice questions covering cell structure and function, genetics, evolution, human anatomy
and physiology, microbiology, and ecology, with a focus on nursing-relevant concepts. Se-
lect the best answer from the four options provided. An answer key with explanations is
included.
• Time Limit: Approximately 60–80 minutes, reflecting HESI A2 Biology section
pacing.
• Materials: No calculator required.
• Scoring: Each question is worth 1 point. No penalty for incorrect answers.
2 Biology Practice Questions (80 Questions)
1. What is the primary function of mitochondria in a cell?
a) Protein synthesis
b) Energy production
c) Cell division
d) Waste storage
Answer: b) Mitochondria produce ATP, the cells energy currency.
2. Which organ system regulates body temperature?
a) Nervous
b) Integumentary
c) Endocrine
d) Muscular
Answer: b) The integumentary system (skin) helps regulate temperature.
3. What is the role of DNA in a cell?
a) Energy storage
b) Genetic information storage
c) Cell membrane protection
d) Protein transport
Answer: b) DNA stores genetic instructions for cell functions.
4. Which blood vessel carries oxygenated blood from the lungs to the heart?
a) Pulmonary artery
b) Pulmonary vein
c) Aorta
d) Vena cava
Answer: b) The pulmonary vein carries oxygenated blood.
5. What is the basic unit of heredity?
a) Chromosome
b) Gene
2
