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Intro to Cell Biology

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Immunofluorescence is a powerful microscopy technique that uses fluorescent markers to visualize specific cellular structures and proteins. It involves the use of antibodies to target particular antigens, with secondary antibodies tagged with fluorophores to emit visible signals. This method allows scientists to determine protein locations within cells. One of its key advantages is the flexibility in color selection, enabling the simultaneous labeling of multiple proteins in a single image. Indirect immunofluorescence enhances precision by strategically marking cellular components. Different fluorophores emit various colors, such as green, red, and far-red, and can be attached to proteins through immunostaining methods.

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Uploaded on
May 26, 2025
Number of pages
4
Written in
2024/2025
Type
Class notes
Professor(s)
Dr. alexis lainoff
Contains
All classes

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Intro to the Cells
Cell Theory:
• All living organism are composed of one or more cells
• Cells are the smallest living thing
• Cells can. Only arise from the division of previous existing
cells
Microscope Invention:
• 100AD: Glass Invented in
Rome
• 1250AD: Wearable lenses
(Spectacles) invented in
Italy and England
• 1608: Lipperhey and Janssen
(spectacle makers) created
the compound microscope
- MAJOR INNOVATION
• 1660s: Using this, Robert T
Hooke discovered "cells" in
cork
• Allowed the fundamental results which is the discovery of
cells and its organelles
• Light microscope alone is insufficient to resolve many cellular
structures
Fluorescence Microscopy:
• Can show us the location of specific molecule in the cell
• Confocal: Uses laser to show a single plane in the image
• It works by manipulating the position of electrons from↳
one. Orbital to another
• Electrons in a fluorophore can be "excited" by specific
wavelengths of light
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