Microbiology Module 3 BIOD 171D
Questions and Answers
AAnanometerAisAdefinedAas:A-ACORRECTAANSWERSA-A.A10-3B.A10-6C.10-9D.A10-12
TrueAorAFalse:AAAnanometerisAlongerAthanAaAmicrometer.A-ACORRECTAANSWERSA-
False.AAAnanometerAisA1,000AtimesAsmallerAthanAaAmicrometer.
ResolutionAandAcontrastAareAtwoAcriticalAfactorsAthatAinfluenceAyourAabilityAtoAseeAanAobject.AExpl
ainAeach.A-ACORRECTAANSWERSA-
ResolutionArefersAtoAtheAdistanceAbetweenAtwoAobjectsAatAwhichAtheAobjectsAstillAcanAbeAseenAasAs
eparate.APoorAorAlowAresolutionAmeansAtwoA(orAmore)AobjectsAmayAappearAasAone.AContrastAisAthe
AdifferenceAinAlightAabsorbanceAbetweenAtwoAobjects.APoorAcontrastAgivesAaAhighAbackgroundAand
AmakesAtheAvisualizationAofAmultipleAobjectsAdifficult.AForAinstance,AtryingAtoAidentifyA2AdarkAcol
oredAobjectsAatAnightA(lowAlightA=AlowAcontrast)AversusAtheAsameA2AobjectsAinAtheAmiddleAofAaAsun
nyAafternoonA(brightAlightAagainstA2AdarkAobjectsA=AhighAcontrast).
AssumingAaAconstantA(non-
adjustable)AlightAsourceApower,AidentifyAtheApartAofAtheAmicroscopeAyouAwouldAadjustAtoAlimitAthe
AamountAofAlightAenteringAtheAmicroscope.ASelectAallAthatAapply.AA.AObjectiveAB.ACondenserAC.AIr
isAdiaphragmAD.AEyeApieceA-ACORRECTAANSWERSA-
C.TheAirisAcontrolsAtheAamountAofAlightAthatApassesAthroughAtheAsampleAandAintoAtheAobjectiveAle
ns.AThus,AitAcanAbeAadjustedA(openedAorAclosed)AtoAalterAtheAamountAofAlight.
WhatAisAtheAtotalAmagnificationA(relativeAtoAyourAeye)AofAaAsampleAimagedAwithAaA60xAobjectiveA
andAaA10xAeyepiece?AShowAyourAmath.A-ACORRECTAANSWERSA-60AxA10A=A600xAmagnification
TrueAorAFalse:AStainingAisAoftenArequiredAtoAimageAaAcellAthatAisAadherentAandAflatA(thin).A-
ACORRECTAANSWERSA-
True.AAdherent,AflatAandAunstainedAcellsAareAalmostAinvisibleAdueAtoAtheAlimitsAonAbothAresolution
AandAcontrast.ATherefore, AcellAstainingAisAoftenArequiredAtoAadequatelyAimageAtheAsample.
WhichAofAtheAfollowingAcouldANOTAbeAseenAclearlyAbyAtheAunaidedAeye?ASelectAallAthatAapply.AA
.ABacteriaAwithAdiameterAofA24AμmAB.AProtozoaAwithAdiameterAofA150AμmAC.AVirusAwithAaAdiamet
erAofA0.2AμmAD.ASkinAcellAwithAdiameterAofA1500AμmA-ACORRECTAANSWERSA-
AAandAC.ATheAunaidedAeyeAcan,AonAaverage,AclearlyAresolveAobjectsA>A100Aμm
LabelAtheAfollowingAunmarkedAmicroscopeAcomponentsA(numberedAarrows)AbyAmatchingAitAwithA
theAcomponentsAprovidedA(letters).AA.AStageAB.AFineAAdjustmentAKnobAC.AIrisADiaphragmAD.ANe
ckAE.ACondenserALensAF.AEyepieceAG.AObjectiveAH.ABaseAI.ACoaxialAControlsA-
ACORRECTAANSWERSA-1FA2DA3BA4GA5AA6H1.
ThisAtypeAofAmicroscopeAutilizesAultravioletA(UV)AlightAtoAilluminateAstainedAobjects.A-
ACORRECTAANSWERSA-Fluorescence
ThisAtypeAofAmicroscopeAusesAaAspecializedAcondenserAandAobjectiveAtoAamplifyAtheAslightAdiffere
ncesAbetweenAcellsAandAbackground.A-ACORRECTAANSWERSA-Phase-Contrast
ThisAtypeAofAmicroscopeAenhancesAcontrastAbetweenAspecimenAandAbackgroundAbutAdoesAnotAper
mitAtheAvisualizationAofAintracellularAstructures.A-ACORRECTAANSWERSA-DarkAField
ThisAtypeAofAmicroscopeAusesAneitherAhalogenAnorAUVAlightAsourcesAbutAratherAlasersAtoAillumina
teAstainedAcellsAinAhighAresolution.A-ACORRECTAANSWERSA-Confocal
Questions and Answers
AAnanometerAisAdefinedAas:A-ACORRECTAANSWERSA-A.A10-3B.A10-6C.10-9D.A10-12
TrueAorAFalse:AAAnanometerisAlongerAthanAaAmicrometer.A-ACORRECTAANSWERSA-
False.AAAnanometerAisA1,000AtimesAsmallerAthanAaAmicrometer.
ResolutionAandAcontrastAareAtwoAcriticalAfactorsAthatAinfluenceAyourAabilityAtoAseeAanAobject.AExpl
ainAeach.A-ACORRECTAANSWERSA-
ResolutionArefersAtoAtheAdistanceAbetweenAtwoAobjectsAatAwhichAtheAobjectsAstillAcanAbeAseenAasAs
eparate.APoorAorAlowAresolutionAmeansAtwoA(orAmore)AobjectsAmayAappearAasAone.AContrastAisAthe
AdifferenceAinAlightAabsorbanceAbetweenAtwoAobjects.APoorAcontrastAgivesAaAhighAbackgroundAand
AmakesAtheAvisualizationAofAmultipleAobjectsAdifficult.AForAinstance,AtryingAtoAidentifyA2AdarkAcol
oredAobjectsAatAnightA(lowAlightA=AlowAcontrast)AversusAtheAsameA2AobjectsAinAtheAmiddleAofAaAsun
nyAafternoonA(brightAlightAagainstA2AdarkAobjectsA=AhighAcontrast).
AssumingAaAconstantA(non-
adjustable)AlightAsourceApower,AidentifyAtheApartAofAtheAmicroscopeAyouAwouldAadjustAtoAlimitAthe
AamountAofAlightAenteringAtheAmicroscope.ASelectAallAthatAapply.AA.AObjectiveAB.ACondenserAC.AIr
isAdiaphragmAD.AEyeApieceA-ACORRECTAANSWERSA-
C.TheAirisAcontrolsAtheAamountAofAlightAthatApassesAthroughAtheAsampleAandAintoAtheAobjectiveAle
ns.AThus,AitAcanAbeAadjustedA(openedAorAclosed)AtoAalterAtheAamountAofAlight.
WhatAisAtheAtotalAmagnificationA(relativeAtoAyourAeye)AofAaAsampleAimagedAwithAaA60xAobjectiveA
andAaA10xAeyepiece?AShowAyourAmath.A-ACORRECTAANSWERSA-60AxA10A=A600xAmagnification
TrueAorAFalse:AStainingAisAoftenArequiredAtoAimageAaAcellAthatAisAadherentAandAflatA(thin).A-
ACORRECTAANSWERSA-
True.AAdherent,AflatAandAunstainedAcellsAareAalmostAinvisibleAdueAtoAtheAlimitsAonAbothAresolution
AandAcontrast.ATherefore, AcellAstainingAisAoftenArequiredAtoAadequatelyAimageAtheAsample.
WhichAofAtheAfollowingAcouldANOTAbeAseenAclearlyAbyAtheAunaidedAeye?ASelectAallAthatAapply.AA
.ABacteriaAwithAdiameterAofA24AμmAB.AProtozoaAwithAdiameterAofA150AμmAC.AVirusAwithAaAdiamet
erAofA0.2AμmAD.ASkinAcellAwithAdiameterAofA1500AμmA-ACORRECTAANSWERSA-
AAandAC.ATheAunaidedAeyeAcan,AonAaverage,AclearlyAresolveAobjectsA>A100Aμm
LabelAtheAfollowingAunmarkedAmicroscopeAcomponentsA(numberedAarrows)AbyAmatchingAitAwithA
theAcomponentsAprovidedA(letters).AA.AStageAB.AFineAAdjustmentAKnobAC.AIrisADiaphragmAD.ANe
ckAE.ACondenserALensAF.AEyepieceAG.AObjectiveAH.ABaseAI.ACoaxialAControlsA-
ACORRECTAANSWERSA-1FA2DA3BA4GA5AA6H1.
ThisAtypeAofAmicroscopeAutilizesAultravioletA(UV)AlightAtoAilluminateAstainedAobjects.A-
ACORRECTAANSWERSA-Fluorescence
ThisAtypeAofAmicroscopeAusesAaAspecializedAcondenserAandAobjectiveAtoAamplifyAtheAslightAdiffere
ncesAbetweenAcellsAandAbackground.A-ACORRECTAANSWERSA-Phase-Contrast
ThisAtypeAofAmicroscopeAenhancesAcontrastAbetweenAspecimenAandAbackgroundAbutAdoesAnotAper
mitAtheAvisualizationAofAintracellularAstructures.A-ACORRECTAANSWERSA-DarkAField
ThisAtypeAofAmicroscopeAusesAneitherAhalogenAnorAUVAlightAsourcesAbutAratherAlasersAtoAillumina
teAstainedAcellsAinAhighAresolution.A-ACORRECTAANSWERSA-Confocal