BSC 2010 Lab Quiz 6-2 study guide
Bacterial DNA Ans✓✓✓ Double stranded, double helix, circular DNA
structure
Medium Size
Containing essential genes for cell metabolism
Have a cell membrane
Bacterial Plasmid DNA Ans✓✓✓ Double stranded, double helix, extra-
chromosomal circular DNA structure
Small in size
Contains additional genes for special functions, such as antibiotic
resistance
Have a cell membrane
DNA can be degraded by enzymes called _____ Ans✓✓✓ nucleases
Empty vectors Ans✓✓✓ contain restriction sites that can be cut to
allow foreign DNA to be inserted within it
*Do NOT carry foreign DNA*
Engineered plasmids Ans✓✓✓ contain restriction sites that can be cut
to allow foreign DNA to be inserted within it
, How do nucleases work? Ans✓✓✓ Bind DNA using a divalent cation
cofactor
Isopropyl alcohol and ethanol Ans✓✓✓ Precipitates DNA, DNA is not
soluable in alcohol
Lysozyme Ans✓✓✓ An enzyme that degrades cell membranes and
Peptidoglycan, exposing the DNA
Mammalian DNA Ans✓✓✓ Double stranded, double helix, linear DNA
structure
Large in size
Have a nuclear/Cell membrane
Media Ans✓✓✓ The liquid solution in which the bacteria was grown in
Microcentrifuge Ans✓✓✓ Separates materials based on density
Non-Specific Ans✓✓✓ Cuts DNA anywhere and will degrade your DNA
samples
Pellet Ans✓✓✓ After spinning the tube, this is the solid at the bottom
Bacterial DNA Ans✓✓✓ Double stranded, double helix, circular DNA
structure
Medium Size
Containing essential genes for cell metabolism
Have a cell membrane
Bacterial Plasmid DNA Ans✓✓✓ Double stranded, double helix, extra-
chromosomal circular DNA structure
Small in size
Contains additional genes for special functions, such as antibiotic
resistance
Have a cell membrane
DNA can be degraded by enzymes called _____ Ans✓✓✓ nucleases
Empty vectors Ans✓✓✓ contain restriction sites that can be cut to
allow foreign DNA to be inserted within it
*Do NOT carry foreign DNA*
Engineered plasmids Ans✓✓✓ contain restriction sites that can be cut
to allow foreign DNA to be inserted within it
, How do nucleases work? Ans✓✓✓ Bind DNA using a divalent cation
cofactor
Isopropyl alcohol and ethanol Ans✓✓✓ Precipitates DNA, DNA is not
soluable in alcohol
Lysozyme Ans✓✓✓ An enzyme that degrades cell membranes and
Peptidoglycan, exposing the DNA
Mammalian DNA Ans✓✓✓ Double stranded, double helix, linear DNA
structure
Large in size
Have a nuclear/Cell membrane
Media Ans✓✓✓ The liquid solution in which the bacteria was grown in
Microcentrifuge Ans✓✓✓ Separates materials based on density
Non-Specific Ans✓✓✓ Cuts DNA anywhere and will degrade your DNA
samples
Pellet Ans✓✓✓ After spinning the tube, this is the solid at the bottom
