APPLIED SCIENCE UNIT 2 LATEST EXAM 2025
WITH MULTIPLE CHOICE OF QUESTIONS AND
DETAILED CORRECT ANSWERS ALREADY
GRADED A+ AND 100% GUARANTEE PASS
(BRAND NEW!!!!!)
Suggest why laboratory technicians must complete health and
safety training before they are allowed to work in the laboratory
- CORRECT ANSWER-to ensure that they are aware of the risk and
hazards in laboratories and how to deal with them.
Information that must be recorded for samples taken - CORRECT
ANSWER-date
name of person handling samples
location (where sample is from and where it is transported to)
Flame test colours - CORRECT ANSWER-Barium (Ba2+)= Pale Green
Copper= Green Blue
Lithium= Crimson Red
Sodium (Na+)= Yellow
Potassium (K+)= Lilac
Calcium (Ca2+)= Orange-red
,Properties of a suitable internal standard - CORRECT ANSWER-
Internal standard is chemically related to the compounds that
are being analysed but it should not be naturally present in the
sample.
Compounds which have the same functional groups.
Why are tubes containing blood samples enclosed in plastic
watertight containers - CORRECT ANSWER-Prevents degradation by
water.
Two containers limits possibility of loss sample if one container
is broken.
2 ways of reducing risks associated with hazards in labs -
CORRECT ANSWER-Carry out risk assessment
Use PPE for protection
Train staff in necessary procedures
Comparing light and electron microscopes - CORRECT ANSWER-
Light Microscope
•Rely on light rays
•Typically magnify objects by 1000 to 2000 X
, •Images seen in 2D
•Live specimen can be seen
Electron Microscope
•Electrons bombarded using high levels of energy
•Specimen is killed
•Colours cannot be detected
•Magnification roughly 2,000,000 X
How can the width of human hair be calculated using a
graticule and a Light Microscope - CORRECT ANSWER-•Mount hair
on Microscope slide,use sellotape to hold the hair down.
•Measure number of divisions corresponding to width of hair
on graticule.
•Look through graticule at a calibration slide to find number of
divisions on calibration slide corresponding to divisions on
graticule.
•Calculate the true distance corresponding to divisions.
•Calculate width of hair.
Explain how Gel Electrophoresis can be carried out in a school
or college laboratory - CORRECT ANSWER-•Prepare the agarose gel
tray, place this tray into Electrophoresis chamber.
•Submerge gel using diluted electrophoresis buffer.
•Load wells with dye samples and place safety cover.
•Connect wires to the power source and begin electrophoresis
by loading current through the gel.
WITH MULTIPLE CHOICE OF QUESTIONS AND
DETAILED CORRECT ANSWERS ALREADY
GRADED A+ AND 100% GUARANTEE PASS
(BRAND NEW!!!!!)
Suggest why laboratory technicians must complete health and
safety training before they are allowed to work in the laboratory
- CORRECT ANSWER-to ensure that they are aware of the risk and
hazards in laboratories and how to deal with them.
Information that must be recorded for samples taken - CORRECT
ANSWER-date
name of person handling samples
location (where sample is from and where it is transported to)
Flame test colours - CORRECT ANSWER-Barium (Ba2+)= Pale Green
Copper= Green Blue
Lithium= Crimson Red
Sodium (Na+)= Yellow
Potassium (K+)= Lilac
Calcium (Ca2+)= Orange-red
,Properties of a suitable internal standard - CORRECT ANSWER-
Internal standard is chemically related to the compounds that
are being analysed but it should not be naturally present in the
sample.
Compounds which have the same functional groups.
Why are tubes containing blood samples enclosed in plastic
watertight containers - CORRECT ANSWER-Prevents degradation by
water.
Two containers limits possibility of loss sample if one container
is broken.
2 ways of reducing risks associated with hazards in labs -
CORRECT ANSWER-Carry out risk assessment
Use PPE for protection
Train staff in necessary procedures
Comparing light and electron microscopes - CORRECT ANSWER-
Light Microscope
•Rely on light rays
•Typically magnify objects by 1000 to 2000 X
, •Images seen in 2D
•Live specimen can be seen
Electron Microscope
•Electrons bombarded using high levels of energy
•Specimen is killed
•Colours cannot be detected
•Magnification roughly 2,000,000 X
How can the width of human hair be calculated using a
graticule and a Light Microscope - CORRECT ANSWER-•Mount hair
on Microscope slide,use sellotape to hold the hair down.
•Measure number of divisions corresponding to width of hair
on graticule.
•Look through graticule at a calibration slide to find number of
divisions on calibration slide corresponding to divisions on
graticule.
•Calculate the true distance corresponding to divisions.
•Calculate width of hair.
Explain how Gel Electrophoresis can be carried out in a school
or college laboratory - CORRECT ANSWER-•Prepare the agarose gel
tray, place this tray into Electrophoresis chamber.
•Submerge gel using diluted electrophoresis buffer.
•Load wells with dye samples and place safety cover.
•Connect wires to the power source and begin electrophoresis
by loading current through the gel.
