MLS ASCP Microbiology UPDATED
ACTUAL Exam Questions and CORRECT
Answers
1 : 10 ratio - CORRECT ANSWER - The proper blood-to-broth ratio for blood cultures to
reduce the antibacterial effects of serum
Beta-lactamase - CORRECT ANSWER - Produced by a penicillin-resistant N.
gonorrhoeae
Columbia CNA with 5% sheep blood - CORRECT ANSWER - Selective medium used for
the isolation of gram-positive microorgnisms
Indole test - CORRECT ANSWER - used for determining an organism's ability to produce
indole from deamination of tryptophan
JEMBEC system - CORRECT ANSWER - is a transport and inoculation medium used for
direct plating of specimens for N gonorrhoeae. Chocolate based selective medium is inoculated
with the specimen. This is placed in an impermeable plastic bag with a bicarbonate-sodium
citrate pellet to generate a carbon dioxide rich environment.
Martin Lewis agar - CORRECT ANSWER - Chocolate agar base containing vancomycin,
colistin, anisomycin, and trimethoprim for better inhibition of gram-positive organisms and
candida.
Differential medium - CORRECT ANSWER - aids in the presumptive identification of
organisms based on their apperance
SPS - CORRECT ANSWER - used as anticoagulant in blood cultures to inactivate
neutrophils and components of serum complement
, Blood culture - CORRECT ANSWER - Typhoid fever can be confirmed by this culture
Regan-Lowe agar - CORRECT ANSWER - best medium for culture for Bordetella
pertussis
Francisella tularensis - CORRECT ANSWER - most rapid method for detection is PCR
synovial infections - CORRECT ANSWER - Anaerobic susceptibility testing is helpful
Schlichter test - CORRECT ANSWER - is used to monitor bactericidal activity during
antimicrobic therapy in cases of endocaditis
Moraxella osloensis - CORRECT ANSWER - is a gram-negative coccobacillus that is
often plump and occurs in pairs and demonstrates a morphology similar to Neisseria
Extra-intestinal isolates of Salmonella - CORRECT ANSWER - should be tested for
resistance to nalidixic acid and if resistant report the ciprofloxacin as resistant
Gentamicin - CORRECT ANSWER - antimicrobial resistance occurs from enzymatic drug
modification
Enterococcus infection - CORRECT ANSWER - need 2 antimicrobial agents, penicillin
derivative and an aminoglycoside
pulsed-field gel electrophoresis - CORRECT ANSWER - commonly used method to
determine the relatedness of 2 or more bacterial strains
Micrococcus - CORRECT ANSWER - is modified oxidase positive, bacitracin (0.04U)
susceptible and resistant to lysotaphin
ACTUAL Exam Questions and CORRECT
Answers
1 : 10 ratio - CORRECT ANSWER - The proper blood-to-broth ratio for blood cultures to
reduce the antibacterial effects of serum
Beta-lactamase - CORRECT ANSWER - Produced by a penicillin-resistant N.
gonorrhoeae
Columbia CNA with 5% sheep blood - CORRECT ANSWER - Selective medium used for
the isolation of gram-positive microorgnisms
Indole test - CORRECT ANSWER - used for determining an organism's ability to produce
indole from deamination of tryptophan
JEMBEC system - CORRECT ANSWER - is a transport and inoculation medium used for
direct plating of specimens for N gonorrhoeae. Chocolate based selective medium is inoculated
with the specimen. This is placed in an impermeable plastic bag with a bicarbonate-sodium
citrate pellet to generate a carbon dioxide rich environment.
Martin Lewis agar - CORRECT ANSWER - Chocolate agar base containing vancomycin,
colistin, anisomycin, and trimethoprim for better inhibition of gram-positive organisms and
candida.
Differential medium - CORRECT ANSWER - aids in the presumptive identification of
organisms based on their apperance
SPS - CORRECT ANSWER - used as anticoagulant in blood cultures to inactivate
neutrophils and components of serum complement
, Blood culture - CORRECT ANSWER - Typhoid fever can be confirmed by this culture
Regan-Lowe agar - CORRECT ANSWER - best medium for culture for Bordetella
pertussis
Francisella tularensis - CORRECT ANSWER - most rapid method for detection is PCR
synovial infections - CORRECT ANSWER - Anaerobic susceptibility testing is helpful
Schlichter test - CORRECT ANSWER - is used to monitor bactericidal activity during
antimicrobic therapy in cases of endocaditis
Moraxella osloensis - CORRECT ANSWER - is a gram-negative coccobacillus that is
often plump and occurs in pairs and demonstrates a morphology similar to Neisseria
Extra-intestinal isolates of Salmonella - CORRECT ANSWER - should be tested for
resistance to nalidixic acid and if resistant report the ciprofloxacin as resistant
Gentamicin - CORRECT ANSWER - antimicrobial resistance occurs from enzymatic drug
modification
Enterococcus infection - CORRECT ANSWER - need 2 antimicrobial agents, penicillin
derivative and an aminoglycoside
pulsed-field gel electrophoresis - CORRECT ANSWER - commonly used method to
determine the relatedness of 2 or more bacterial strains
Micrococcus - CORRECT ANSWER - is modified oxidase positive, bacitracin (0.04U)
susceptible and resistant to lysotaphin
