MICR 290 EXAM
PREP/COMPREHENSIVE STUDY
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Terms in this set (178)
, Cloning refers to the process of
inserting a sequence of DNA (also
called an insert) into a plasmid. The
resulting plasmid is a form of
recombinant DNA, meaning that it is
an artificial (i.e. laboratory-
produced) construct combining
DNA sequences from two or more
different sources.
It refers to the process of inserting a
sequence of DNA into a vector. The
insert may come from various
What is cloning? sources (e.g. from DNA purified
from bacteria), but is most
commonly produced by polymerase
chain reaction.
Plasmids used for cloning are known
as cloning vectors, and are designed
to simplify the process of
transferring an insert into the empty
vector. If the goal is to produce a
protein encoded by the insert DNA
(i.e. the insert is a gene), a specific
, kind of cloning vector known as an
expression vector must be used.
Circular, double-stranded DNA
What are the present in the bacterial cytoplasm
correct features
of a plasmid? Many plasmids carry genes that
confer antibiotic resistance
Plasmids are extremely valuable
tools for biotechnology
applications, and recombinant
plasmids are used in many different
areas of research. By inserting a
sequence of DNA into a plasmid
and transforming a bacterial culture
with this recombinant DNA, the
Why are culture can then be used as a
plasmids useful? "factory" to produce a massive
number of copies of the plasmid.
This is one way to produce copies of
the DNA sequence that was inserted
into the plasmid (i.e. the insert). The
resulting plasmid can then be
purified from the bacterial culture
(e.g. using spin columns) and used in
down-stream applications.
, The plasmids used in the lab for
cloning purposes are known as
vectors, as they act as "vectors" for
the insert that they carry.
Many of the plasmids used for
research purposes are artificial
constructs, combining DNA
sequences from several different
sources (including natural bacterial
plasmids).
What are Most empty vectors are relatively
vectors? small, typically ranging from 2-5 kb
(kilobases) in size; this contrasts
natural plasmids, which are often 10-
100 kb. Cloning vectors can be
considered to be "stripped down"
versions of natural plasmids,
consisting principally of the core
components required for plasmid
replication and selection. As long as
these components are not
disrupted, it is possible to clone
almost any insert DNA sequence
into a plasmid.
PREP/COMPREHENSIVE STUDY
GUIDE 2025 | QUESTIONS AND
CORRECT DETAILED ANSWERS |
GRADED A+ | VERIFIED ANSWERS |
JUST RELEASED
Save
Terms in this set (178)
, Cloning refers to the process of
inserting a sequence of DNA (also
called an insert) into a plasmid. The
resulting plasmid is a form of
recombinant DNA, meaning that it is
an artificial (i.e. laboratory-
produced) construct combining
DNA sequences from two or more
different sources.
It refers to the process of inserting a
sequence of DNA into a vector. The
insert may come from various
What is cloning? sources (e.g. from DNA purified
from bacteria), but is most
commonly produced by polymerase
chain reaction.
Plasmids used for cloning are known
as cloning vectors, and are designed
to simplify the process of
transferring an insert into the empty
vector. If the goal is to produce a
protein encoded by the insert DNA
(i.e. the insert is a gene), a specific
, kind of cloning vector known as an
expression vector must be used.
Circular, double-stranded DNA
What are the present in the bacterial cytoplasm
correct features
of a plasmid? Many plasmids carry genes that
confer antibiotic resistance
Plasmids are extremely valuable
tools for biotechnology
applications, and recombinant
plasmids are used in many different
areas of research. By inserting a
sequence of DNA into a plasmid
and transforming a bacterial culture
with this recombinant DNA, the
Why are culture can then be used as a
plasmids useful? "factory" to produce a massive
number of copies of the plasmid.
This is one way to produce copies of
the DNA sequence that was inserted
into the plasmid (i.e. the insert). The
resulting plasmid can then be
purified from the bacterial culture
(e.g. using spin columns) and used in
down-stream applications.
, The plasmids used in the lab for
cloning purposes are known as
vectors, as they act as "vectors" for
the insert that they carry.
Many of the plasmids used for
research purposes are artificial
constructs, combining DNA
sequences from several different
sources (including natural bacterial
plasmids).
What are Most empty vectors are relatively
vectors? small, typically ranging from 2-5 kb
(kilobases) in size; this contrasts
natural plasmids, which are often 10-
100 kb. Cloning vectors can be
considered to be "stripped down"
versions of natural plasmids,
consisting principally of the core
components required for plasmid
replication and selection. As long as
these components are not
disrupted, it is possible to clone
almost any insert DNA sequence
into a plasmid.
