Microbiology Chapter 9 Questions And
Answers
The bfollowing bare bsteps bused bto bmake bDNA bfingerprints. bWhat bis bthe bthird bstep?
A) bcollect bDNA
B) bdigest bwith ba brestriction benzyme
C) bperform belectrophoresis
D) blyse bcells
E) badd bstain b- bcorrect banswers✔✔digest bwith ba brestriction benzyme
how bmany bpieces bwill bEcoRI bproduce bfrom bthe bplasmid bshown bin bFigure b9.1?
A) b1 b
B) b2
C) b3
D) b4
E) b5 b- bcorrect banswers✔✔2
In bFigure b9.1, bafter bdigestion bwith bthe bappropriate brestriction benzyme, bwhat bis bthe
bsmallest bpiece bcontaining bthe bentire bampicillin-resistance b(amp) bgene?
A) b0.17 bkbp
B) b0.25 bkbp
C) b1.08 bkbp
D) b1.50 bkbp
E) b3.00 bkbp b- bcorrect banswers✔✔1.50 bkbp
In bFigure b9.2, bthe benzyme bin bstep b1 bis
A) bDNA bpolymerase.
B) bDNA bligase.
C) bRNA bpolymerase.
D) breverse btranscriptase.
E) bspliceosome. b- bcorrect banswers✔✔RNA bpolymerase
In bFigure b9.2, bthe benzyme bin bstep b2 bis
A) bDNA bpolymerase.
B) bDNA bligase.
C) bRNA bpolymerase.
D) breverse btranscriptase.
E) bspliceosome. b- bcorrect banswers✔✔DNA bpolymerase
, The breaction bcatalyzed bby breverse btranscriptase bis
A) bDNA b→ bmRNA.
B) bmRNA b→ bcDNA.
C) bmRNA b→ bprotein.
D) bDNA b→ bDNA.
E) btRNA b→ bmRNA. b- bcorrect banswers✔✔mRNA b-> bprotein
Which bof bthe bfollowing bis ban badvantage bof busing bE. bcoli bto bmake ba bhuman bgene
bproduct?
A) bEndotoxin bmay bbe bin bthe bproduct.
B) bIt bdoes bnot bsecrete bmost bproteins.
C) bIts bgenes bare bwell bknown.
D) bIt bcannot bprocess bintrons.
E) bEndotoxin bmay bbe bin bthe bproduct band bit bdoes bnot bsecrete bmost bproteins. b-
bcorrect banswers✔✔its bgenes bare bwell bknown
Which bof bthe bfollowing bis bNOT ban bagricultural bproduct bmade bby bDNA btechniques?
A) bfrost bretardant
B) bBacillus bthuringiensis binsecticide
C) bnitrogenase b(nitrogen bfixation)
D) bglyphosate-resistant bcrops
E) bpectinase b- bcorrect banswers✔✔pectinase
If byou bhave binserted ba bgene bin bthe bTi bplasmid, bthe bnext bstep bin bgenetic
bengineering bis
A) btransformation bof bE. bcoli bwith bTi bplasmid.
B) bsplicing bT bDNA binto ba bplasmid.
C) btransformation bof ban banimal bcell.
D) binserting bthe bTi bplasmid binto bAgrobacterium.
E) binserting bthe bTi bplasmid binto ba bplant bcell. b- bcorrect banswers✔✔inserting bthe bTi
bplasmid binto bAgrobacterium
Biotechnology binvolves bthe
A) buse bof bmicroorganisms bto bmake bdesired bproducts.
B) buse bof banimal bcells bto bmake bvaccines.
C) bdevelopment bof bdisease-resistant bcrop bplants.
D) buse bof bmicroorganisms bto bmake bdesired bproducts band bthe buse bof banimal bcells
bto bmake bvaccines.
E) buse bof bmicroorganisms bto bmake bdesired bproducts, bthe buse bof banimal bcells bto
bmake bvaccines, band bthe bdevelopment bof bdisease-resistant bcrop bplants. b- bcorrect
banswers✔✔use bthe bmicroorganisms bto bmake bdesired bproducts, bthe buse bof banimal
bcells bto bmake bvaccines, band bthe bdevelopment bof bdisease-resistant bcrop bplants
The bfigure bat bthe bleft bin bFigure b9.3 bshows ba bgene bidentified bby bSouthern bblotting.
bWhat bwill ba bSouthern bblot bof bthe bsame bgene blook blike bafter bPCR?
A) ba
Answers
The bfollowing bare bsteps bused bto bmake bDNA bfingerprints. bWhat bis bthe bthird bstep?
A) bcollect bDNA
B) bdigest bwith ba brestriction benzyme
C) bperform belectrophoresis
D) blyse bcells
E) badd bstain b- bcorrect banswers✔✔digest bwith ba brestriction benzyme
how bmany bpieces bwill bEcoRI bproduce bfrom bthe bplasmid bshown bin bFigure b9.1?
A) b1 b
B) b2
C) b3
D) b4
E) b5 b- bcorrect banswers✔✔2
In bFigure b9.1, bafter bdigestion bwith bthe bappropriate brestriction benzyme, bwhat bis bthe
bsmallest bpiece bcontaining bthe bentire bampicillin-resistance b(amp) bgene?
A) b0.17 bkbp
B) b0.25 bkbp
C) b1.08 bkbp
D) b1.50 bkbp
E) b3.00 bkbp b- bcorrect banswers✔✔1.50 bkbp
In bFigure b9.2, bthe benzyme bin bstep b1 bis
A) bDNA bpolymerase.
B) bDNA bligase.
C) bRNA bpolymerase.
D) breverse btranscriptase.
E) bspliceosome. b- bcorrect banswers✔✔RNA bpolymerase
In bFigure b9.2, bthe benzyme bin bstep b2 bis
A) bDNA bpolymerase.
B) bDNA bligase.
C) bRNA bpolymerase.
D) breverse btranscriptase.
E) bspliceosome. b- bcorrect banswers✔✔DNA bpolymerase
, The breaction bcatalyzed bby breverse btranscriptase bis
A) bDNA b→ bmRNA.
B) bmRNA b→ bcDNA.
C) bmRNA b→ bprotein.
D) bDNA b→ bDNA.
E) btRNA b→ bmRNA. b- bcorrect banswers✔✔mRNA b-> bprotein
Which bof bthe bfollowing bis ban badvantage bof busing bE. bcoli bto bmake ba bhuman bgene
bproduct?
A) bEndotoxin bmay bbe bin bthe bproduct.
B) bIt bdoes bnot bsecrete bmost bproteins.
C) bIts bgenes bare bwell bknown.
D) bIt bcannot bprocess bintrons.
E) bEndotoxin bmay bbe bin bthe bproduct band bit bdoes bnot bsecrete bmost bproteins. b-
bcorrect banswers✔✔its bgenes bare bwell bknown
Which bof bthe bfollowing bis bNOT ban bagricultural bproduct bmade bby bDNA btechniques?
A) bfrost bretardant
B) bBacillus bthuringiensis binsecticide
C) bnitrogenase b(nitrogen bfixation)
D) bglyphosate-resistant bcrops
E) bpectinase b- bcorrect banswers✔✔pectinase
If byou bhave binserted ba bgene bin bthe bTi bplasmid, bthe bnext bstep bin bgenetic
bengineering bis
A) btransformation bof bE. bcoli bwith bTi bplasmid.
B) bsplicing bT bDNA binto ba bplasmid.
C) btransformation bof ban banimal bcell.
D) binserting bthe bTi bplasmid binto bAgrobacterium.
E) binserting bthe bTi bplasmid binto ba bplant bcell. b- bcorrect banswers✔✔inserting bthe bTi
bplasmid binto bAgrobacterium
Biotechnology binvolves bthe
A) buse bof bmicroorganisms bto bmake bdesired bproducts.
B) buse bof banimal bcells bto bmake bvaccines.
C) bdevelopment bof bdisease-resistant bcrop bplants.
D) buse bof bmicroorganisms bto bmake bdesired bproducts band bthe buse bof banimal bcells
bto bmake bvaccines.
E) buse bof bmicroorganisms bto bmake bdesired bproducts, bthe buse bof banimal bcells bto
bmake bvaccines, band bthe bdevelopment bof bdisease-resistant bcrop bplants. b- bcorrect
banswers✔✔use bthe bmicroorganisms bto bmake bdesired bproducts, bthe buse bof banimal
bcells bto bmake bvaccines, band bthe bdevelopment bof bdisease-resistant bcrop bplants
The bfigure bat bthe bleft bin bFigure b9.3 bshows ba bgene bidentified bby bSouthern bblotting.
bWhat bwill ba bSouthern bblot bof bthe bsame bgene blook blike bafter bPCR?
A) ba
