MIC319 FINAL EXAM QUESTIONS WITH
ACCURATE ANSWERS
False on the consequence of not having NK cells
a. Easily succumb to viral infection
b. Higher incidence of cancer
c. Cell mediated killing of cells expressing high levels of MMC-I in the cell surface
d. Impaired anti-viral innate immunity - ANSWER c. Cell mediated killing of cells
expressing high levels of MMC-I in the cell surface
Indicate false about evasion of the immune response by viruses
a. Many viruses carry mechanism to downregulate the MMC Class-! To avoid immune
recognition.
b. Evasion of the innate immune response by viruses can prevent all disease symptoms.
c. Many pathogenic viruses carry anti-apoptotic proteins
d. Viruses have evolved mechanism to evade killing by NK cells - ANSWER b. Evasion of
the innate immune response by viruses can prevent all disease symptoms.
Identify the false statement about the apoptosome and it formation
a. Cytochrome C released by the mitochondria catalyzes the oligomerization of APAF
with Caspase-9 to form the apoptosome
b. It can be directly activated by death receptors via DISC formation and activation of
Caspase-8
c. It is a molecular killing machine that massively activates Caspase-3
d. It is antagonized by anti-apoptotic IAP proteins - ANSWER b. It can be directly
activated by death receptors via DISC formation and activation of Caspase-8
Indicate False about TNF-alpha, an inflammatory cytokine that in spite of binding a
receptor containing a dead domain does not cause apoptosis in many cell types:
a. TNF-alpha treated cells survive because TNF-alpha can't activate the extrinsic
apoptosis pathway through DISC formation
b. TNF-alpha activation of the NFKB pathway leads to transcription of the anti apoptotic
Bcl-2 member Bcl-XL and the IAP apoptosome inhibitor.
,c. The TNF-alpha receptor activates DISC leading to caspase 8 activation
d. TNF-alpha treated cells can show cleavage of caspase 3 - ANSWER a. TNF-alpha
treated cells survive because TNF-alpha can't activate the extrinsic apoptosis pathway
through DISC formation
Indicate the only mechanism unique to the extrinsic pathway of apoptosis
a. Death receptor recruitment of the adaptor FADD and caspase 8 with formation of the
DISC signaling complex
b. Mitochondrial release of Cytochrome C leading to apoptosome formation
c. Cleavage of pro caspase 3
d. Phagocytosis of apoptotic bodies - ANSWER a. Death receptor recruitment of the
adaptor FADD and caspase 8 with formation of the DISC signaling complex
True about symptoms of viral disease
a. Could be caused by direct cytotoxicity of viruses on host cells
b. Caused by undesirable effects of the innate immune response
c. Could be caused by uncontrolled inflammatory responses to viruses
d. All answers provided for this questions are correct - ANSWER d. All answers provided
for this questions are correct
Which is the best explanation about the nature of the viral gene inactivated by the
mutation?
a. It regulates the transcription of an anti-apoptotic MCMC viral gene
b. It inhibits inflammasome formation by MCMV DNA
c. Inhibits induction of the Type 1 IFN response.
d. It inhibits host cell protein synthesis - ANSWER a. It regulates the transcription of an
anti-apoptotic MCMC viral gene
Identify the statement that is not true about apoptosis
a. It leads to massive DAMP release and inflammation
b. It is an active, programmed process that requires ATP
c. It leads to blebbing and apoptotic body formation
d. Apoptotic bodies express eat me signals in the cell surface - ANSWER d. Apoptotic
bodies express eat me signals in the cell surface
,Identify the false statement about Bcl-2 family of proteins:
a. Anti-apoptotic members such as Bcl-2 members antagonize pore formation by
pro-apoptotic members Bax and Bak that would lead to release of Cytochrome C
b. They are the key component of the apoptosome
c. Some pro-apoptotic members such as Bid and its cleavage product, tbid, are a way
whereby the extrinsic pathway can regulate the intrinsic pathway
d. Anti-apoptotic members can be upregulated by NfkB - ANSWER b. They are the key
component of the apoptosome
Identify the least inflammatory among the following mechanisms of programmed cell
death
a. Necrosis
b. Apoptosis
c. Pyroptosis
d. Necroptosis - ANSWER b. Apoptosis
When an infected cell is committed to die the best way of dying is:
a. The one that release as much DAMPS as possible such as necrosis leading to
uncontrolled inflammation
b. Dying with the least inflammation possible
c. With the right extent of inflammation associated with the dying depending on the virus
and the strength of infection and response
d. All answers provided for this questions are correct - ANSWER c. With the right extent
of inflammation associated with the dying depending on the virus and the strength of
infection and response
Which is the only false statement about mechanisms of cell death
a. Necrosis promotes inflammation by membrane rupture and release of DAMPs
b. Necroptosis is a non-inflammatory way of programmed cell death
c. Autophagy is a type of programmed cell death implicated in processing endocytosed
virus leading to viral PAMP presentation
d. Pyroptosis causes inflammatory cell death by activation of caspase 1 which leads to
apoptosome formation and cleavage of pro-IL-1beta. - ANSWER b. Necroptosis is a
non-inflammatory way of programmed cell death
, Identify the false statement for properties of Natural killer cells
a. Are activated by Type 1 Interferons
b. Are designed to kill virally infected cells that down regulate the MMC class 1 and
show stress related NK receptor ligands in the surface
c. Can kill cells by formation of pores and delivery of caspase like molecules
d. They can't kill cells using their death ligand and the engagement of the extrinsic
pathway - ANSWER d. They can't kill cells using their death ligand and the engagement
of the extrinsic pathway
Indicate False about mechanisms of NK recognition of infected cells and activation
a. NK cells do not kill normal cells because the MMC-I binds and block the NK activating
receptor containing ITAM motifs
b. Expression of high levels of NK receptor ligand, provides one of the necessary signals
for cells to be killed by NK cells by activating NK activating receptors containing ITAM
motifs
c. NK do not kill cells expressing high levels of MMC-I such as the normal cells, because
MMC-I molecules bind and activate signaling by NK inhibitory receptors containing ITIM
motifs
d. Absence of MMC I expression is necessary but it is not sufficient for a cell to be killed
by an NK cell - ANSWER a. NK cells do not kill normal cells because the MMC-I binds and
block the NK activating receptor containing ITAM motifs
Indicate False about mechanisms for NK killing of infected cells
a. A poly Perforin 1 pore is necessary to transport Granzymes A and B to the target cell
ACCURATE ANSWERS
False on the consequence of not having NK cells
a. Easily succumb to viral infection
b. Higher incidence of cancer
c. Cell mediated killing of cells expressing high levels of MMC-I in the cell surface
d. Impaired anti-viral innate immunity - ANSWER c. Cell mediated killing of cells
expressing high levels of MMC-I in the cell surface
Indicate false about evasion of the immune response by viruses
a. Many viruses carry mechanism to downregulate the MMC Class-! To avoid immune
recognition.
b. Evasion of the innate immune response by viruses can prevent all disease symptoms.
c. Many pathogenic viruses carry anti-apoptotic proteins
d. Viruses have evolved mechanism to evade killing by NK cells - ANSWER b. Evasion of
the innate immune response by viruses can prevent all disease symptoms.
Identify the false statement about the apoptosome and it formation
a. Cytochrome C released by the mitochondria catalyzes the oligomerization of APAF
with Caspase-9 to form the apoptosome
b. It can be directly activated by death receptors via DISC formation and activation of
Caspase-8
c. It is a molecular killing machine that massively activates Caspase-3
d. It is antagonized by anti-apoptotic IAP proteins - ANSWER b. It can be directly
activated by death receptors via DISC formation and activation of Caspase-8
Indicate False about TNF-alpha, an inflammatory cytokine that in spite of binding a
receptor containing a dead domain does not cause apoptosis in many cell types:
a. TNF-alpha treated cells survive because TNF-alpha can't activate the extrinsic
apoptosis pathway through DISC formation
b. TNF-alpha activation of the NFKB pathway leads to transcription of the anti apoptotic
Bcl-2 member Bcl-XL and the IAP apoptosome inhibitor.
,c. The TNF-alpha receptor activates DISC leading to caspase 8 activation
d. TNF-alpha treated cells can show cleavage of caspase 3 - ANSWER a. TNF-alpha
treated cells survive because TNF-alpha can't activate the extrinsic apoptosis pathway
through DISC formation
Indicate the only mechanism unique to the extrinsic pathway of apoptosis
a. Death receptor recruitment of the adaptor FADD and caspase 8 with formation of the
DISC signaling complex
b. Mitochondrial release of Cytochrome C leading to apoptosome formation
c. Cleavage of pro caspase 3
d. Phagocytosis of apoptotic bodies - ANSWER a. Death receptor recruitment of the
adaptor FADD and caspase 8 with formation of the DISC signaling complex
True about symptoms of viral disease
a. Could be caused by direct cytotoxicity of viruses on host cells
b. Caused by undesirable effects of the innate immune response
c. Could be caused by uncontrolled inflammatory responses to viruses
d. All answers provided for this questions are correct - ANSWER d. All answers provided
for this questions are correct
Which is the best explanation about the nature of the viral gene inactivated by the
mutation?
a. It regulates the transcription of an anti-apoptotic MCMC viral gene
b. It inhibits inflammasome formation by MCMV DNA
c. Inhibits induction of the Type 1 IFN response.
d. It inhibits host cell protein synthesis - ANSWER a. It regulates the transcription of an
anti-apoptotic MCMC viral gene
Identify the statement that is not true about apoptosis
a. It leads to massive DAMP release and inflammation
b. It is an active, programmed process that requires ATP
c. It leads to blebbing and apoptotic body formation
d. Apoptotic bodies express eat me signals in the cell surface - ANSWER d. Apoptotic
bodies express eat me signals in the cell surface
,Identify the false statement about Bcl-2 family of proteins:
a. Anti-apoptotic members such as Bcl-2 members antagonize pore formation by
pro-apoptotic members Bax and Bak that would lead to release of Cytochrome C
b. They are the key component of the apoptosome
c. Some pro-apoptotic members such as Bid and its cleavage product, tbid, are a way
whereby the extrinsic pathway can regulate the intrinsic pathway
d. Anti-apoptotic members can be upregulated by NfkB - ANSWER b. They are the key
component of the apoptosome
Identify the least inflammatory among the following mechanisms of programmed cell
death
a. Necrosis
b. Apoptosis
c. Pyroptosis
d. Necroptosis - ANSWER b. Apoptosis
When an infected cell is committed to die the best way of dying is:
a. The one that release as much DAMPS as possible such as necrosis leading to
uncontrolled inflammation
b. Dying with the least inflammation possible
c. With the right extent of inflammation associated with the dying depending on the virus
and the strength of infection and response
d. All answers provided for this questions are correct - ANSWER c. With the right extent
of inflammation associated with the dying depending on the virus and the strength of
infection and response
Which is the only false statement about mechanisms of cell death
a. Necrosis promotes inflammation by membrane rupture and release of DAMPs
b. Necroptosis is a non-inflammatory way of programmed cell death
c. Autophagy is a type of programmed cell death implicated in processing endocytosed
virus leading to viral PAMP presentation
d. Pyroptosis causes inflammatory cell death by activation of caspase 1 which leads to
apoptosome formation and cleavage of pro-IL-1beta. - ANSWER b. Necroptosis is a
non-inflammatory way of programmed cell death
, Identify the false statement for properties of Natural killer cells
a. Are activated by Type 1 Interferons
b. Are designed to kill virally infected cells that down regulate the MMC class 1 and
show stress related NK receptor ligands in the surface
c. Can kill cells by formation of pores and delivery of caspase like molecules
d. They can't kill cells using their death ligand and the engagement of the extrinsic
pathway - ANSWER d. They can't kill cells using their death ligand and the engagement
of the extrinsic pathway
Indicate False about mechanisms of NK recognition of infected cells and activation
a. NK cells do not kill normal cells because the MMC-I binds and block the NK activating
receptor containing ITAM motifs
b. Expression of high levels of NK receptor ligand, provides one of the necessary signals
for cells to be killed by NK cells by activating NK activating receptors containing ITAM
motifs
c. NK do not kill cells expressing high levels of MMC-I such as the normal cells, because
MMC-I molecules bind and activate signaling by NK inhibitory receptors containing ITIM
motifs
d. Absence of MMC I expression is necessary but it is not sufficient for a cell to be killed
by an NK cell - ANSWER a. NK cells do not kill normal cells because the MMC-I binds and
block the NK activating receptor containing ITAM motifs
Indicate False about mechanisms for NK killing of infected cells
a. A poly Perforin 1 pore is necessary to transport Granzymes A and B to the target cell
