Biochemistry - C785 Module 3 & 4

BIOCHEMISTRY - C785 MODULE 3 &
4


2,3-BPGfstabilizesfwhichfstatefoffhemoglobin?f-fanswers--Tfstate

2,3-
BPGfstabilizesfthefTfstatefoffhemoglobin.fThisfreducesfhemoglobinfaffinityfforf_________
_fandfpromotesfO2f________toftheftissuesf-fanswers--O2,freleased

COfstabilizesfwhichfstatefoffhemoglobin?f-fanswers--Rfstate

WhenfCOfbindsftofthefsitefatfthefironfhemoglobinfitfchangesfthatfheme'sf____________f-
fanswers--shapefandfchangesfintofthefRfstate


H+(protons)fstabilizesfwhichfstatefoffhemoglobin?f-fanswers--Tfstate

AtfaflowerfpHfoffanfactivelyfmetabolizingftissue,fhemoglobinfhasfaf__________faffinityfforf
oxygenf-fanswers--LOWER

LowfO2,fLowfpHf-fanswers--1.fHighfH+
2.fHighfCO2
3.fAcidic
4.fTfStatef-fbentf
5.fDeoxygenated

HighfO2,fhighfpHf-fanswers--1.fBasic
2.fhemoglobinfbindsftofoxygen
3.fplanarfRfstate
4.fdecreasesfthefaffinityfforfoxygen

Bohrfeffectf-fanswers--1.fCarbonfdioxidefdecreasesfhemoglobinsfaffinityfforfoxygen
2.fwhenfH+fionsfincrease,fphfdecrease
3.fwhenfpHfisflowfaffinityfforfoxygenfandfreleasesfoxygen

Hemoglobinfbindsftofthefoxygenfatfaf___________f-fanswers--HighfpH

Hemoglobinfreleasesfoxygenfatfaf______________f-fanswers--LowfpH

Myoglobinf-fanswers--1.fstoresfoxygen

, 2.fhasfafhigherfaffinityfforfoxygen
3.fhasfaftertiaryfstructure
4.fmonomerf-fsinglefpolypeptidefchain

Hemoglobinf-fanswers--1.ftransportsfoxygen
2.fhasf4fFEf(II)fatomsftofbindf4foxygenfmolecules
3.fhasftertiaryfstructure

Hemoglobinfcanfbindfveryftightlyftofoxygenfinfthef-fanswers--
lungs,fwherefoxygenflevelsfarefhigh

Hemoglobinfreleasefoxygenfinftheftissuesf-fanswers--
wherefthesefoxygenflevelsfarefdecreased

Hemoglobin'sfcooperativefbindingfbehaviorfisfinfluencedfbyfbindingf____________f-
fanswers--oxygen


Sigmoidalf(s-shape)f-fanswers--hemoglobin

Curveftofthefleftf-fanswers--equalsfafbase
highfaffinityfforfoxygen

Curvesftofthefrightf-fanswers--equalsfanfacid
lowerfaffinityfforfoxygen

Hyperbolicf(sideways,fufshape)f-fanswers--myoglobin

Deoxygentatedf-fanswers--Tfstate

Oxygenatedf-fanswers--Rfstate

Deoxyhemoglobinf-fanswers--oxygenfunbound,fTfstate

Tfstate,fdeoxygenatedf-fanswers--1.flowfpH
2.flowfconcentrationfoffoxygen
3.fhighfconcentrationfoffH+f(protons)
4.fhighfconcentrationfoffCO2
5.fhemefisfbentf(nonfplanar)

MyoglobinfandfHemoglobinfbothfbindsf-fanswers--oxygenfviafthefhemefgroup

Whyfdoesfthefsubunitfcompositionfoffhemoglobinfmakefitfanfidealfcandidateftoftransportfo
xygen?f-fanswers--
Sincefhemoglobinfhasf4fsubunits,fitfcanftransportffourfoxygenfmolecules.fAdditionally,fco
mmunicationfbetweenftheffourfsubunitsf(cooperativity)fwillfensurefthatfallffourfsubunitsfpic
kfupfandfdeliverfoxygen.