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C785 BIOCHEMISTRY PREASSESSMENT

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Exam (elaborations)

C785 Biochemistry Preassessment

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2024/2025

Institution C785

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C785 BIOCHEMISTRY PREASSESSMENT

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C785 BIOCHEMISTRY PREASSESSMENT

Assumingf100%freactionfefficiency,fhowfmanyfDNAfcopiesfarefcreatedfafterfthefcompletio
nfofffourfcompletefPCRfcycles?f-fanswers--16

WhatfisftheffunctionfoffDNAfpolymerasefinfthefprocessfoffPCR?f-fanswers--
ItfrecognizesfthefprimersfandfusesfthefavailablefdNTPsftofreplicateftheftemplatefDNAfseq
uence.

WhatfarefpossiblefbloodftypesfoffthefparentsfoffafpatientfwithfbloodftypefAB?f-fanswers--
AfandfB

115f116f117f118f−fAminofAcidfNumberf
TyrfGlyfThrfProf−fAminofAcidfSequencef
TACfGGGfACAfCCAf−fDNAfSequencef

Whatfisfthefaminofacidfinfpositionf118fthatfresultsffromfafsinglefnucleotidefinsertionfimmed
iatelyffollowingfthefthreefguaninesfthatfencodefforfglycinef116?f-fanswers--Thr

Infsickle-
cellfdisease,fthefglutamicfacidf(Glu)fatfpositionfnumberfsevenfisfmutatedftofvalinef(Val).f

Whatfchangefinfcodonsfcorrespondsftofthisfmutation?f-fanswers--GAAftofGTA

WhichftypefoffmammalianfDNAfdamagefrepairfrequiresfthefpresencefoffthefchromosomeft
hatfisfhomologousftofthefdamagedfchromosome?f-fanswers--Recombination

Xerodermafpigmentosumf(XP)fisfafrecessivefgeneticfdiseasefthatfoccursfwhenfoneforfmor
efoffthefgenesfthatfperformfnucleotidefexcisionfrepairfarefnonfunctional.f

WhyfdofXPfpatientsfhavefafmuchfhigherfincidencefoffskinfcancerfthanfthefgeneralfpopulati
on?f-fanswers--Thefmutationfratefoffallfotherfgenesfisfhigherfdueftoffailureftofrepair.

WhichfmRNAfsequencefresultsfinfafpolypeptidefthatfcontainsfthreefaminofacids?f-
fanswers--5'f-fCUUfGAUfACUfUAGf-f3'

WhichftemplatefstrandfDNAfsequencefwouldfresultfinfthefaminofacidf(Ser)?f-fanswers--5'f-
fCGAf-f3'

, WhatfisfthefcomplementaryfsequencefforftheffollowingfDNAfsequence?f

5'f-fGCCfATCfTCGfAATf-f3'f-fanswers--5'f-fATTfCGAfGATfGGCf-f3'

StudiesfhavefshownfthatfincreasedfexpressionfofftwofgenesfcalledfSIRT1fandfSIRT2fisfas
sociatedfwithfchronicfexposureftofcocainefandfcontributesftofdrugfaddiction.f

WhatfdifferencefinfthefregionsfoffthefSIRT1fandfSIRT2fgenesfinfpeoplefaddictedftofcocain
efincreasesftheirfexpression?f-fanswers--Thefnucleosomesfbecomefmorefwidelyfspaced.

WhatfoccursfduringfthefprocessfoffalternativefsplicingfoffmRNA?f-fanswers--
Alternatefcombinationsfoffexonsfwithinfthefsamefgenefareflinkedftogether.

Whichfforcefisfmostfinfluentialfinfdeterminingftheftertiaryfstructurefoffafprotein?f-fanswers--
Hydrophobicfeffect

Givenftheffollowingfaminofacidfstructure,fwhatfisfthefstrongestfintermolecularfforcefitfwoul
dfparticipatefinftofstabilizefafproteinfstructure?f-fanswers--Hydrogenfbond

Whatfisfthefnamefofflarge,ffunctionalfproteinfstructuresfcomposedfoffsmallerfproteinsfwithf
multiplefsubunitsf-fanswers--Quaternaryfstructure

Whichfstructuralflevelfshowsfthefgreatestfdifferencefwhenfreplacingfdenaturedfproteinsfwit
hfafdenaturedfsinglefsubunitfprotein?f-fanswers--Tertiary

Whichflevelfoffproteinfstructurefisfestablishedfthroughfthefdehydrationfsynthesisfoffpeptide
fbonds?f-fanswers--primary

Whichfsituationfwouldfmostflikelyfleadftofafpermanentfalterationfoffproteinffunction?f-
fanswers--
Afmutationfthatfsubstitutesfafhydrophobicfaminofacidfwithfafhydrophilicfaminofacid

Givenftheffollowingfaminofacidfstructure,fwhatfisfthefstrongestfintermolecularfforcefitfwoul
dfparticipatefinftofstabilizefafproteinfstructure?f-fanswers--hydrogenfbond

Whatfisfthefnamefofflarge,ffunctionalfproteinfstructuresfcomposedfoffsmallerfproteinsfwithf
multiplefsubunits?f-fanswers--quarternaryfstructure

Pepsin,fthefenzymefthatfcatalyzesfthefbreakingfoffpeptidefbonds,fhasfthefhighestfactivityfi
nfthefacidicfenvironmentfoffthefstomach.f

WhatfprocessfisfdisruptedfafterfafpatientfingestsfexcessivefantacidfthatfneutralizesfthefpHf
offthefstomach?f-fanswers--Proteinfcatabolism

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Uploaded on: April 29, 2025
Number of pages: 5
Written in: 2024/2025
Type: Exam (elaborations)
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